2021
Platelet-derived growth factor receptor beta activates Abl2 via direct binding and phosphorylation
Wu K, Wu H, Lyu W, Kim Y, Furdui CM, Anderson KS, Koleske AJ. Platelet-derived growth factor receptor beta activates Abl2 via direct binding and phosphorylation. Journal Of Biological Chemistry 2021, 297: 100883. PMID: 34144039, PMCID: PMC8259415, DOI: 10.1016/j.jbc.2021.100883.Peer-Reviewed Original ResearchConceptsAbl family kinasesFamily kinasesPlatelet-derived growth factor receptor betaGrowth factor receptor betaAbl familySrc homology 2 domainSrc homology 3 domainDiverse cellular stimuliPost-translational modificationsN-terminal halfNonreceptor tyrosine kinaseMultiple novel sitesAutoinhibited conformationSrc homologyCytoskeleton organizationCytoplasmic domainCellular stimuliKinase domainGrowth factor receptorReceptor betaKinase activityMolecular mechanismsTyrosine kinaseDirect bindingKinase
2018
Abl2 is recruited to ventral actin waves through cytoskeletal interactions to promote lamellipodium extension
Zhang K, Lyu W, Yu J, Koleske AJ. Abl2 is recruited to ventral actin waves through cytoskeletal interactions to promote lamellipodium extension. Molecular Biology Of The Cell 2018, 29: 2863-2873. PMID: 30256707, PMCID: PMC6249870, DOI: 10.1091/mbc.e18-01-0044.Peer-Reviewed Original ResearchConceptsCytoskeletal interactionsLamellipodium extensionTotal internal reflection fluorescence microscopyActin-rich structuresActin wavesActin-rich protrusionsN-terminal halfC-terminal halfNonreceptor tyrosine kinaseReflection fluorescence microscopyFoci colocalizeComplementation analysisLamellipodia protrusionKnockout cellsLamellipodium tipActin filament stabilizerCell shapeBind actinTyrosine kinaseCortactinABL2Fluorescence microscopyIntegrin β3High spatiotemporal resolutionPaxillin
2015
Direct Interactions with the Integrin β1 Cytoplasmic Tail Activate the Abl2/Arg Kinase*
Simpson MA, Bradley WD, Harburger D, Parsons M, Calderwood DA, Koleske AJ. Direct Interactions with the Integrin β1 Cytoplasmic Tail Activate the Abl2/Arg Kinase*. Journal Of Biological Chemistry 2015, 290: 8360-8372. PMID: 25694433, PMCID: PMC4375489, DOI: 10.1074/jbc.m115.638874.Peer-Reviewed Original ResearchConceptsIntegrin β1 cytoplasmic tailExtracellular matrix adhesion receptorsSrc homology domainFibroblast cell motilityIntegrin β1Β1 cytoplasmic tailMembrane-proximal segmentAdhesion complex formationMatrix adhesion receptorsNonreceptor tyrosine kinaseArg kinase activityArg nonreceptor tyrosine kinaseCancer cell invasivenessHomology domainActin cytoskeletonCytoplasmic tailCytoskeletal remodelingDendrite morphogenesisTyr-783Kinase domainPhosphorylated regionAbl familyΒ1 tailArg kinaseCell motility
2013
Arg kinase signaling in dendrite and synapse stabilization pathways: Memory, cocaine sensitivity, and stress
Kerrisk ME, Koleske AJ. Arg kinase signaling in dendrite and synapse stabilization pathways: Memory, cocaine sensitivity, and stress. The International Journal Of Biochemistry & Cell Biology 2013, 45: 2496-2500. PMID: 23916785, PMCID: PMC3797846, DOI: 10.1016/j.biocel.2013.07.018.Peer-Reviewed Original ResearchConceptsNeuronal actin cytoskeletonPhosphorylation of substratesNonreceptor tyrosine kinaseArg nonreceptor tyrosine kinaseActin cytoskeletonPostnatal mouse brainDendritic spinesArg kinaseMolecular mechanismsF-actinTyrosine kinaseSignaling pathwaysPostnatal day 21Synapse stabilityIntegrin α3β1KinaseNeurodegenerative diseasesNeuronal remodelingTherapeutic approachesCocaine sensitivityStabilization pathwayDay 21Arbor sizeMouse brainArgc-ABL Gene and Gene Product
Rosenberg B, Koleske A. c-ABL Gene and Gene Product. 2013, 401-403. DOI: 10.1016/b978-0-12-374984-0.00185-6.Peer-Reviewed Original ResearchC-AblDNA-binding domainDNA damage responseNonreceptor tyrosine kinaseC-terminal cassetteC-abl geneVertebrate paralogsDamage response
2012
Lysozyme contamination facilitates crystallization of a heterotrimeric cortactin–Arg–lysozyme complex
Liu W, MacGrath SM, Koleske AJ, Boggon TJ. Lysozyme contamination facilitates crystallization of a heterotrimeric cortactin–Arg–lysozyme complex. Acta Crystallographica Section F: Structural Biology Communications 2012, 68: 154-158. PMID: 22297987, PMCID: PMC3274391, DOI: 10.1107/s1744309111056132.Peer-Reviewed Original ResearchConceptsCrystal structure determinationNonreceptor tyrosine kinaseArg nonreceptor tyrosine kinaseHeterotrimeric complexSH3 domainMacromolecular crystallographersCrystallography approachStructure determinationTyrosine kinaseCocrystal structureMolecular replacementTrace amountsLysozyme complexStructure solutionProteinCortactinComplexesCrystallizationCrystallographersCocrystalsKinaseMotifAutomatic model buildingSequenceArg
2011
Cell adhesion signaling pathways: First responders to cocaine exposure?
Gourley SL, Taylor JR, Koleske AJ. Cell adhesion signaling pathways: First responders to cocaine exposure? Communicative & Integrative Biology 2011, 4: 30-3. PMID: 21509173, PMCID: PMC3073265, DOI: 10.4161/cib.4.1.14083.Peer-Reviewed Original ResearchCocaine exposureDendritic spinesDendritic spine structureChronic drug exposureCertain brain regionsExpression/functionDrug exposurePsychomotor sensitizationPsychostimulant exposureBrain regionsFirst respondersNeuronal shapeSpine structureHuman brainRespondersExposureSpineTyrosine kinaseRecent findingsNonreceptor tyrosine kinaseCell adhesion receptorsAdhesion receptorsDevelopmental periodAdhesion factorsCell adhesion factorsCell adhesion signaling pathways
Gourley S, Taylor J, Koleske A. Cell adhesion signaling pathways. Communicative & Integrative Biology 2011, 4: 30-33. DOI: 10.4161/cib.14083.Peer-Reviewed Original ResearchDendritic spinesDendritic spine structureChronic drug exposureCertain brain regionsExpression/functionDrug exposureCocaine exposurePsychomotor sensitizationPsychostimulant exposureBrain regionsNeuronal shapeSpine structureHuman brainExposureSpineTyrosine kinaseRecent findingsNonreceptor tyrosine kinaseCell adhesion receptorsAdhesion receptorsDevelopmental periodAdhesion factorsCell adhesion factorsFirst respondersCytoskeletal effectors
2008
The c-Abl tyrosine kinase regulates actin remodeling at the immune synapse
Huang Y, Comiskey EO, Dupree RS, Li S, Koleske AJ, Burkhardt JK. The c-Abl tyrosine kinase regulates actin remodeling at the immune synapse. Blood 2008, 112: 111-119. PMID: 18305217, PMCID: PMC2435682, DOI: 10.1182/blood-2007-10-118232.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdaptor Proteins, Signal TransducingAnimalsCells, CulturedHumansInterleukin-2Jurkat CellsLymphocyte ActivationMiceMice, KnockoutPhosphorylationProtein BindingProteinsProto-Oncogene Proteins c-ablPseudopodiaReceptors, Antigen, T-CellSignal TransductionT-LymphocytesTranscription, GeneticWiskott-Aldrich Syndrome Protein FamilyConceptsC-AblImmune synapseActin responseC-Abl nonreceptor tyrosine kinaseT cell activationTyrosine kinaseC-Abl tyrosine kinaseC-Abl bindsActin regulatory proteinsNonreceptor tyrosine kinaseChemokine-induced T cell migrationT cell receptor engagementSH2 domainActin dynamicsActin reorganizationTyrosine phosphorylationRegulatory proteinsActin polymerizationDownstream eventsNormal localizationConditional knockout miceCoordinate actionReceptor engagementKinaseLamellipodial spreading
2007
A Critical Role for Cortactin Phosphorylation by Abl-Family Kinases in PDGF-Induced Dorsal-Wave Formation
Boyle SN, Michaud GA, Schweitzer B, Predki PF, Koleske AJ. A Critical Role for Cortactin Phosphorylation by Abl-Family Kinases in PDGF-Induced Dorsal-Wave Formation. Current Biology 2007, 17: 445-451. PMID: 17306540, DOI: 10.1016/j.cub.2007.01.057.Peer-Reviewed Original ResearchConceptsAbl family kinasesCortactin phosphorylationActin regulatory protein cortactinTyrosine kinaseAbl family tyrosine kinasesSrc family kinasesNonreceptor tyrosine kinaseHuman protein microarrayCell morphogenesisActin reorganizationCytoskeletal rearrangementsProtein cortactinGrowth factor receptorLamellipodial protrusionCytoskeletal structuresCell motilityProper regulationPDGF treatmentTyrosine residuesCortactinKinaseNovel substrateDownstream actionsPhosphorylationProtein microarrays
2006
Regulation of Cytoskeletal Dynamics and Cell Morphogenesis by Abl Family Kinases
Koleske A. Regulation of Cytoskeletal Dynamics and Cell Morphogenesis by Abl Family Kinases. Molecular Biology Intelligence Unit 2006, 48-67. DOI: 10.1007/978-0-387-68744-5_5.Peer-Reviewed Original ResearchCell morphogenesisProtrusive structuresActin rich protrusive structuresFormation of actinAbl family kinasesNonreceptor tyrosine kinaseExtended C-terminusMetazoan organismsArp2/3 complexCytoskeletal dynamicsProtein complexesRac GTPasesExhibit defectsFamily kinasesNeuronal morphogenesisAbl familyCytoskeletal rearrangementsCytoskeletal networkCytoskeletal structuresEssential regulatorMutant animalsC-terminusActin filamentsTyrosine kinaseF-actin
2005
Integrin-Mediated Dendrite Branch Maintenance Requires Abelson (Abl) Family Kinases
Moresco EM, Donaldson S, Williamson A, Koleske AJ. Integrin-Mediated Dendrite Branch Maintenance Requires Abelson (Abl) Family Kinases. Journal Of Neuroscience 2005, 25: 6105-6118. PMID: 15987940, PMCID: PMC6725048, DOI: 10.1523/jneurosci.1432-05.2005.Peer-Reviewed Original ResearchConceptsCortical dendritesPostnatal day 21Nervous system functionDendrite lossCortical neuronsSemaphorin 7ADay 21Arg kinase activityArbor sizeMouse brainArbor structureDendrite maintenanceEarly adulthoodNeurite branchingTyrosine kinaseNeurite branchesDendritesNonreceptor tyrosine kinaseIntegrin receptorsCritical determinantLaminin-1System functionKinase activityBranch dynamicsDendrite branches
2004
The Abl-related gene (Arg) requires its F-actin–microtubule cross-linking activity to regulate lamellipodial dynamics during fibroblast adhesion
Miller AL, Wang Y, Mooseker MS, Koleske AJ. The Abl-related gene (Arg) requires its F-actin–microtubule cross-linking activity to regulate lamellipodial dynamics during fibroblast adhesion. Journal Of Cell Biology 2004, 165: 407-420. PMID: 15138293, PMCID: PMC2172189, DOI: 10.1083/jcb.200308055.Peer-Reviewed Original ResearchConceptsLamellipodial dynamicsCross-linking activityF-actin-binding domainFluorescent protein fusionsMT-binding domainNonreceptor tyrosine kinaseFilamentous actin bundlesWild-type fibroblastsCell polarityProtein fusionsMembrane protrusionsCell peripheryLamellipodial protrusionCell protrusionsActin bundlesMolecular mechanismsTyrosine kinaseF-actinMicrotubulesArgFibroblast adhesionABLHigh affinityFibroblastsProtrusionBidirectional Signaling Links the Abelson Kinases to the Platelet-Derived Growth Factor Receptor
Plattner R, Koleske AJ, Kazlauskas A, Pendergast AM. Bidirectional Signaling Links the Abelson Kinases to the Platelet-Derived Growth Factor Receptor. Molecular And Cellular Biology 2004, 24: 2573-2583. PMID: 14993293, PMCID: PMC355852, DOI: 10.1128/mcb.24.6.2573-2583.2004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineChemotaxisEnzyme ActivationIn Vitro TechniquesMacromolecular SubstancesMiceNIH 3T3 CellsPhospholipase C gammaPhosphorylationProtein-Tyrosine KinasesProto-Oncogene Proteins c-ablReceptor, Platelet-Derived Growth Factor alphaReceptor, Platelet-Derived Growth Factor betaRecombinant ProteinsSignal TransductionType C PhospholipasesConceptsPDGF beta receptorPlatelet-derived growth factorC-AblPLC-gamma1C-Abl nonreceptor tyrosine kinaseTyrosine kinaseGrowth factor signalsSrc family kinasesC-Abl functionNonreceptor tyrosine kinaseTyrosine kinase activityPlatelet-derived growth factor receptorAbundant phosphoinositideMembrane rufflingInducible complexNonredundant functionsFamily kinasesMembrane proteinsC-gamma1Factor signalsGrowth factor receptorAbelson kinaseKinase activityBiological processesChemotaxis defectHow do Abl family kinases regulate cell shape and movement?
Hernández SE, Krishnaswami M, Miller AL, Koleske AJ. How do Abl family kinases regulate cell shape and movement? Trends In Cell Biology 2004, 14: 36-44. PMID: 14729179, DOI: 10.1016/j.tcb.2003.11.003.Peer-Reviewed Original ResearchConceptsAbl family kinasesFamily kinasesAdhesion receptorsC-terminal halfCytoskeletal regulatory proteinsNonreceptor tyrosine kinaseCell morphogenesisCytoskeletal dynamicsRecent biochemicalCytoskeletal rearrangementsCytoskeletal structuresCytoskeletal componentsRegulatory proteinsCell shapeGenetic analysisTyrosine kinaseKinaseCell surfaceARG proteinRelay signalsProteinLeukemia cellsDrosophilaCrystallographic analysisMorphogenesis
2003
Two Distinct Phosphorylation Pathways Have Additive Effects on Abl Family Kinase Activation
Tanis KQ, Veach D, Duewel HS, Bornmann WG, Koleske AJ. Two Distinct Phosphorylation Pathways Have Additive Effects on Abl Family Kinase Activation. Molecular And Cellular Biology 2003, 23: 3884-3896. PMID: 12748290, PMCID: PMC155218, DOI: 10.1128/mcb.23.11.3884-3896.2003.Peer-Reviewed Original ResearchConceptsSrc nonreceptor tyrosine kinaseAdditive effectTyrosine kinaseArg nonreceptor tyrosine kinaseKinase activationNonreceptor tyrosine kinaseKinase activityCell surface receptorsSurface receptorsStimuli resultsStimulationFivefold stimulationActivationTwofold stimulationABLPhosphorylationPhosphorylation pathwayPathwaySrc family kinase HckSrc familyAbl Family Nonreceptor Tyrosine Kinases Modulate Short-Term Synaptic Plasticity
Moresco EM, Scheetz AJ, Bornmann WG, Koleske AJ, Fitzsimonds RM. Abl Family Nonreceptor Tyrosine Kinases Modulate Short-Term Synaptic Plasticity. Journal Of Neurophysiology 2003, 89: 1678-1687. PMID: 12626632, DOI: 10.1152/jn.00892.2002.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBenzamidesEnzyme InhibitorsHippocampusImatinib MesylateLong-Term PotentiationLong-Term Synaptic DepressionMiceMice, Mutant StrainsMicroscopy, ImmunoelectronNeuronal PlasticityOrgan Culture TechniquesPiperazinesProbabilityProtein-Tyrosine KinasesProto-Oncogene Proteins c-ablPyrimidinesSynapsesSynaptic TransmissionConceptsActin cytoskeletal dynamicsAbl family kinasesNonreceptor tyrosine kinasePaired-pulse facilitationCell morphogenesisMouse hippocampal area CA1Wild-type slicesActin cytoskeletonCytoskeletal dynamicsLong-term depressionLong-term potentiationFamily kinasesAdult mouse brainNonreceptor tyrosineTyrosine kinaseWild-type controlsFunctional interactionPostsynaptic compartmentsPosttetanic potentiationImmunoelectron microscopyMature neuronsImportant functionsKinaseBasal synaptic transmissionABL
2001
The Abl-related gene (Arg) nonreceptor tyrosine kinase uses two F-actin-binding domains to bundle F-actin
Wang Y, Miller A, Mooseker M, Koleske A. The Abl-related gene (Arg) nonreceptor tyrosine kinase uses two F-actin-binding domains to bundle F-actin. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 14865-14870. PMID: 11752434, PMCID: PMC64950, DOI: 10.1073/pnas.251249298.Peer-Reviewed Original ResearchConceptsF-actin-binding domainActin-rich structuresAbl family kinasesNonreceptor tyrosine kinaseF-actinActin cytoskeletonFamily kinasesFluorescent protein fusion proteinTyrosine kinaseActin-bundling activityProtein fusion proteinActin cytoskeletal structuresCellular morphogenesisSwiss 3T3 fibroblastsBundling activityDeletion mutantsCytoskeletal structuresC-terminusFusion proteinFunctional interactionKinaseActin moleculesCytoskeletonPositive cooperativityArgThe ARG Tyrosine Kinase Interacts with Siva-1 in the Apoptotic Response to Oxidative Stress*
Cao C, Ren X, Kharbanda S, Koleske A, Prasad K, Kufe D. The ARG Tyrosine Kinase Interacts with Siva-1 in the Apoptotic Response to Oxidative Stress*. Journal Of Biological Chemistry 2001, 276: 11465-11468. PMID: 11278261, DOI: 10.1074/jbc.c100050200.Peer-Reviewed Original Research