2021
Platelet-derived growth factor receptor beta activates Abl2 via direct binding and phosphorylation
Wu K, Wu H, Lyu W, Kim Y, Furdui CM, Anderson KS, Koleske AJ. Platelet-derived growth factor receptor beta activates Abl2 via direct binding and phosphorylation. Journal Of Biological Chemistry 2021, 297: 100883. PMID: 34144039, PMCID: PMC8259415, DOI: 10.1016/j.jbc.2021.100883.Peer-Reviewed Original ResearchConceptsAbl family kinasesFamily kinasesPlatelet-derived growth factor receptor betaGrowth factor receptor betaAbl familySrc homology 2 domainSrc homology 3 domainDiverse cellular stimuliPost-translational modificationsN-terminal halfNonreceptor tyrosine kinaseMultiple novel sitesAutoinhibited conformationSrc homologyCytoskeleton organizationCytoplasmic domainCellular stimuliKinase domainGrowth factor receptorReceptor betaKinase activityMolecular mechanismsTyrosine kinaseDirect bindingKinase
2013
WAVE2 Regulates Epithelial Morphology and Cadherin Isoform Switching through Regulation of Twist and Abl
Bryce NS, Reynolds AB, Koleske AJ, Weaver AM. WAVE2 Regulates Epithelial Morphology and Cadherin Isoform Switching through Regulation of Twist and Abl. PLOS ONE 2013, 8: e64533. PMID: 23691243, PMCID: PMC3654908, DOI: 10.1371/journal.pone.0064533.Peer-Reviewed Original ResearchConceptsEpithelial morphogenesisAbl kinaseActin cytoskeletal rearrangementCell-cell junctionsE-cadherinRegulation of TwistTotal cell lysatesAbl kinase activityEpithelial morphologyTranscription factor Twist1N-cadherin proteinTwist1 expressionWAVE2 complexABL substratesCellular signalingInhibition of AblCadherin isoformsCytoskeletal rearrangementsTyrosine phosphorylationEpithelial-mesenchymal transitionKinase activityMorphological phenotypesFilament localizationCadherin expressionWAVE2
2008
Enhancement of ABL Kinase Catalytic Efficiency by a Direct Binding Regulator Is Independent of Other Regulatory Mechanisms*
Cao X, Tanis KQ, Koleske AJ, Colicelli J. Enhancement of ABL Kinase Catalytic Efficiency by a Direct Binding Regulator Is Independent of Other Regulatory Mechanisms*. Journal Of Biological Chemistry 2008, 283: 31401-31407. PMID: 18796434, PMCID: PMC2581583, DOI: 10.1074/jbc.m804002200.Peer-Reviewed Original ResearchConceptsSH2 domainKinase activityTyrosine kinaseAbl family tyrosine kinasesKinase activation mechanismRelief of autoinhibitionUnique protein structureFamily tyrosine kinasesCatalytic site mutationsPhosphorylation of CrkInhibitor-resistant mutantsAbl kinase activityTyrosine kinase activitySH3-SH2ABL tyrosine kinase activityABL2 kinaseABL substratesRegulatory domainPhosphorylation mechanismDownstream effectorsDomain coreInactive conformationAbl SH3Regulatory mechanismsProtein structure
2007
The Abl-related Gene Tyrosine Kinase Acts through p190RhoGAP to Inhibit Actomyosin Contractility and Regulate Focal Adhesion Dynamics upon Adhesion to Fibronectin
Peacock JG, Miller AL, Bradley WD, Rodriguez OC, Webb DJ, Koleske AJ. The Abl-related Gene Tyrosine Kinase Acts through p190RhoGAP to Inhibit Actomyosin Contractility and Regulate Focal Adhesion Dynamics upon Adhesion to Fibronectin. Molecular Biology Of The Cell 2007, 18: 3860-3872. PMID: 17652459, PMCID: PMC1995720, DOI: 10.1091/mbc.e07-01-0075.Peer-Reviewed Original ResearchConceptsFocal adhesion dynamicsFocal adhesionsActomyosin contractilityAdhesion dynamicsActin-dependent protrusionsGene Tyrosine KinaseCell migrationCell body translocationAbl family kinasesWild-type cellsC-terminal halfDistinct functional domainsN-terminal halfF-actin stress fibersStress fiber formationInhibits cell migrationKinase inhibitsFamily kinasesCell peripheryStress fibersActin polymerizationFunctional domainsKinase activityTyrosine kinaseWT cells
2005
Integrin-Mediated Dendrite Branch Maintenance Requires Abelson (Abl) Family Kinases
Moresco EM, Donaldson S, Williamson A, Koleske AJ. Integrin-Mediated Dendrite Branch Maintenance Requires Abelson (Abl) Family Kinases. Journal Of Neuroscience 2005, 25: 6105-6118. PMID: 15987940, PMCID: PMC6725048, DOI: 10.1523/jneurosci.1432-05.2005.Peer-Reviewed Original ResearchConceptsCortical dendritesPostnatal day 21Nervous system functionDendrite lossCortical neuronsSemaphorin 7ADay 21Arg kinase activityArbor sizeMouse brainArbor structureDendrite maintenanceEarly adulthoodNeurite branchingTyrosine kinaseNeurite branchesDendritesNonreceptor tyrosine kinaseIntegrin receptorsCritical determinantLaminin-1System functionKinase activityBranch dynamicsDendrite branches
2004
Adhesion-Dependent Regulation of p190RhoGAP in the Developing Brain by the Abl-Related Gene Tyrosine Kinase
Hernández SE, Settleman J, Koleske AJ. Adhesion-Dependent Regulation of p190RhoGAP in the Developing Brain by the Abl-Related Gene Tyrosine Kinase. Current Biology 2004, 14: 691-696. PMID: 15084284, DOI: 10.1016/j.cub.2004.03.062.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsBrainCytoskeletonDNA-Binding ProteinsGTPase-Activating ProteinsGuanine Nucleotide Exchange FactorsImmunoblottingMiceMorphogenesisNeuritesNeuronsNuclear ProteinsPhosphorylationPlasmidsPrecipitin TestsProtein-Tyrosine KinasesRepressor ProteinsTransfectionTumor Cells, CulturedConceptsAdhesion-dependent regulationArg substrateNeuronal morphogenesisKinase activityGene Tyrosine KinaseActin-dependent processesWild-type extractsWild-type cellsArg kinase activityAbl kinase activityMembrane rufflingPostnatal mouse brainFamily kinasesCytoskeletal rearrangementsPhosphotyrosine contentFilopodial protrusionsCell motilityArg kinaseP190RhoGAPMouse brainTyrosine kinaseKinasePhosphorylationMorphogenesisPostnatal brainBidirectional Signaling Links the Abelson Kinases to the Platelet-Derived Growth Factor Receptor
Plattner R, Koleske AJ, Kazlauskas A, Pendergast AM. Bidirectional Signaling Links the Abelson Kinases to the Platelet-Derived Growth Factor Receptor. Molecular And Cellular Biology 2004, 24: 2573-2583. PMID: 14993293, PMCID: PMC355852, DOI: 10.1128/mcb.24.6.2573-2583.2004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell LineChemotaxisEnzyme ActivationIn Vitro TechniquesMacromolecular SubstancesMiceNIH 3T3 CellsPhospholipase C gammaPhosphorylationProtein-Tyrosine KinasesProto-Oncogene Proteins c-ablReceptor, Platelet-Derived Growth Factor alphaReceptor, Platelet-Derived Growth Factor betaRecombinant ProteinsSignal TransductionType C PhospholipasesConceptsPDGF beta receptorPlatelet-derived growth factorC-AblPLC-gamma1C-Abl nonreceptor tyrosine kinaseTyrosine kinaseGrowth factor signalsSrc family kinasesC-Abl functionNonreceptor tyrosine kinaseTyrosine kinase activityPlatelet-derived growth factor receptorAbundant phosphoinositideMembrane rufflingInducible complexNonredundant functionsFamily kinasesMembrane proteinsC-gamma1Factor signalsGrowth factor receptorAbelson kinaseKinase activityBiological processesChemotaxis defect
2003
Two Distinct Phosphorylation Pathways Have Additive Effects on Abl Family Kinase Activation
Tanis KQ, Veach D, Duewel HS, Bornmann WG, Koleske AJ. Two Distinct Phosphorylation Pathways Have Additive Effects on Abl Family Kinase Activation. Molecular And Cellular Biology 2003, 23: 3884-3896. PMID: 12748290, PMCID: PMC155218, DOI: 10.1128/mcb.23.11.3884-3896.2003.Peer-Reviewed Original ResearchConceptsSrc nonreceptor tyrosine kinaseAdditive effectTyrosine kinaseArg nonreceptor tyrosine kinaseKinase activationNonreceptor tyrosine kinaseKinase activityCell surface receptorsSurface receptorsStimuli resultsStimulationFivefold stimulationActivationTwofold stimulationABLPhosphorylationPhosphorylation pathwayPathwaySrc family kinase HckSrc family