2000
Prostaglandin E2 interaction with AVP: effects on AQP2 phosphorylation and distribution
Zelenina M, Christensen B, Palmér J, Nairn A, Nielsen S, Aperia A. Prostaglandin E2 interaction with AVP: effects on AQP2 phosphorylation and distribution. American Journal Of Physiology. Renal Physiology 2000, 278: f388-f394. PMID: 10710543, DOI: 10.1152/ajprenal.2000.278.3.f388.Peer-Reviewed Original ResearchConceptsTranslocation of AQP2AQP2 phosphorylationPlasma membraneAquaporin-2Subcellular distributionPlasma membrane-enriched fractionVesicle-enriched fractionsMembrane-enriched fractionDuct water permeabilityConsensus sitesIntracellular vesiclesPhosphorylationDifferential centrifugation techniqueAction of arginineRenal inner medullaE2 interactionRat renal inner medullaTranslocationInner medullaDose-dependent mannerWater channelsMembraneDephosphorylationTraffickingProtein
1998
Cellular Localization of Calmodulin-dependent Protein Kinases I and II to A-cells and D-cells of the Endocrine Pancreas
Matovcik L, Nairn A, Gorelick F. Cellular Localization of Calmodulin-dependent Protein Kinases I and II to A-cells and D-cells of the Endocrine Pancreas. Journal Of Histochemistry & Cytochemistry 1998, 46: 519-526. PMID: 9524198, DOI: 10.1177/002215549804600412.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCalcium-Calmodulin-Dependent Protein Kinase Type 1Calcium-Calmodulin-Dependent Protein Kinase Type 2Calcium-Calmodulin-Dependent Protein KinasesCyclic AMP Response Element-Binding ProteinCystic Fibrosis Transmembrane Conductance RegulatorDigestive SystemFluorescent Antibody Technique, IndirectGlucagonImmunoblottingIslets of LangerhansMicroscopy, ConfocalPancreasRatsSomatostatinSomatostatin-Secreting CellsTissue DistributionConceptsCaM kinase IKinase IProtein kinase ICaM kinase IIGlucagon secretionKinase IICalmodulin-dependent protein kinase ISomatostatin cellsDependent protein kinase IA cellsSomatostatin-containing D-cellsD cellsDifferent cell typesRegulation of Ca2Islets of LangerhansCaM kinaseCytosolic proteinsI antibodiesSomatostatin granulesPancreatic polypeptideCellular localizationRegulatory rolePeptide antibodiesIntracellular Ca2Endocrine pancreas
1996
Structure, Regulation, and Function of Calcium/Calmodulin-Dependent Protein Kinase I
Picciotto M, Nastiuk K, Nairn A. Structure, Regulation, and Function of Calcium/Calmodulin-Dependent Protein Kinase I. Advances In Pharmacology 1996, 36: 251-275. PMID: 8783563, DOI: 10.1016/s1054-3589(08)60585-2.Peer-Reviewed Original ResearchConceptsProtein kinaseProtein kinase CMyosin light chain kinaseKinase ICaM kinaseSecond messenger-regulated protein kinasesCalmodulin-dependent protein kinase ICalcium/calmodulin-dependent protein kinase ICAMP-dependent protein kinaseSpecific subcellular locationsMultifunctional protein kinaseTerminal regulatory domainDependent protein kinaseCaM kinase familyClass of enzymesProtein kinase ICaM kinase IAmino acid residuesMyosin P-light chainDomain bindsAutoinhibitory mechanismRegulatory domainKinase familyProtein phosphorylationLight chain kinase
1995
Cystic Fibrosis Transmembrane Conductance Regulator Is Found Within Brain Ventricular Epithelium and Choroid Plexus
Hincke M, Nairn A, Staines W. Cystic Fibrosis Transmembrane Conductance Regulator Is Found Within Brain Ventricular Epithelium and Choroid Plexus. Journal Of Neurochemistry 1995, 64: 1662-1668. PMID: 7534334, DOI: 10.1046/j.1471-4159.1995.64041662.x.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorConductance regulatorCystic fibrosis gene productBrain Ventricular EpitheliumCyclic AMP-dependent phosphorylationGene productsCFTR proteinFine punctaChloride transportersCl- channelsCyclic AMP-elevating agentsEpendymal functionWestern blottingRegulatorVentricular epitheliumPhosphorylationChoroid plexusProteinTransportersRodent brainPunctaRegulationMicrodissection
1994
Subcellular localization of CFTR to endosomes in a ductal epithelium
Webster P, Vanacore L, Nairn A, Marino C. Subcellular localization of CFTR to endosomes in a ductal epithelium. American Journal Of Physiology 1994, 267: c340-c348. PMID: 7521124, DOI: 10.1152/ajpcell.1994.267.2.c340.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorEndocytosisEpitheliumFluorescent Antibody TechniqueImmunohistochemistryMaleMembrane ProteinsMicroscopy, FluorescenceOrganellesRatsRats, Sprague-DawleyReceptors, Cell SurfaceSubcellular FractionsSubmandibular GlandTissue DistributionConceptsCystic fibrosis transmembrane conductance regulatorPlasma membraneFibrosis transmembrane conductance regulatorApical plasma membraneAnti-CFTR antibodiesNormal epithelial cell populationsTransmembrane conductance regulatorCytochemical evidenceReceptor-mediated endocytosisCFTR moleculesEpithelial cell populationsCellular processesSubcellular compartmentsSubcellular localizationEarly endosomesMembrane recyclingConductance regulatorSubcellular distributionSubapical vesiclesApical poleEndosomesCFTR functionImmunoelectron microscopyCell populationsCFTR immunoreactivity
1992
Distribution of Protein Phosphatase Inhibitor‐1 in Brain and Peripheral Tissues of Various Species: Comparison with DARPP‐32
Hemmings H, Girault J, Nairn A, Bertuzzi G, Greengard P. Distribution of Protein Phosphatase Inhibitor‐1 in Brain and Peripheral Tissues of Various Species: Comparison with DARPP‐32. Journal Of Neurochemistry 1992, 59: 1053-1061. PMID: 1353788, DOI: 10.1111/j.1471-4159.1992.tb08347.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainCarrier ProteinsCentral Nervous SystemDopamine and cAMP-Regulated Phosphoprotein 32Enzyme InhibitorsFemaleIntracellular Signaling Peptides and ProteinsMaleNerve Tissue ProteinsPhosphoproteinsProteinsRatsRats, Inbred StrainsSubcellular FractionsTissue DistributionTyrosine 3-MonooxygenaseVertebrates
1985
Identification of calmodulin-dependent protein kinase III and its major Mr 100,000 substrate in mammalian tissues.
Nairn A, Bhagat B, Palfrey H. Identification of calmodulin-dependent protein kinase III and its major Mr 100,000 substrate in mammalian tissues. Proceedings Of The National Academy Of Sciences Of The United States Of America 1985, 82: 7939-7943. PMID: 3906654, PMCID: PMC390885, DOI: 10.1073/pnas.82.23.7939.Peer-Reviewed Original ResearchConceptsCaM-dependent protein kinaseCaM kinase IIIKinase IIIProtein kinaseMammalian tissuesCalmodulin-dependent protein kinase IIIProtein kinase IIIDependent protein kinaseProtein phosphorylation systemsWidespread tissue distributionTotal cytosolic proteinAnimal cellsPhosphorylation systemSubstrate specificityCytosolic proteinsMyosin light chainMajor substrateKinaseProteinSynapsin IPoor substrateCell linesPhosphorylase bMajor MrPolyclonal antibodies