2003
GSK-3 kinases enhance calcineurin signaling by phosphorylation of RCNs
Hilioti Z, Gallagher D, Low-Nam S, Ramaswamy P, Gajer P, Kingsbury T, Birchwood C, Levchenko A, Cunningham K. GSK-3 kinases enhance calcineurin signaling by phosphorylation of RCNs. Genes & Development 2003, 18: 35-47. PMID: 14701880, PMCID: PMC314273, DOI: 10.1101/gad.1159204.Peer-Reviewed Original ResearchConceptsProtein phosphataseType 1 protein phosphataseGSK-3 kinaseGSK-3 familyConserved serine residueProtein phosphatase activitySerine residuesAnimal cellsConsensus sitesProtein kinaseMuscle developmentPositive feedback loopYeast cellsRCN1CalcineurinMCIP1Phosphatase activityPhosphorylationEndogenous levelsInhibitory formKinaseLarge inductionStimulatory effectHeart growthPhosphatase
2000
Scaffold proteins may biphasically affect the levels of mitogen-activated protein kinase signaling and reduce its threshold properties
Levchenko A, Bruck J, Sternberg P. Scaffold proteins may biphasically affect the levels of mitogen-activated protein kinase signaling and reduce its threshold properties. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 5818-5823. PMID: 10823939, PMCID: PMC18517, DOI: 10.1073/pnas.97.11.5818.Peer-Reviewed Original ResearchConceptsMitogen-activated protein kinaseScaffold proteinProtein kinaseSpecific cellular contextDifferent subcellular compartmentsDetailed biochemical modelCellular contextSignal transductionSubcellular compartmentsScaffold concentrationsScaffold levelMultimolecular complexesFull activationGeneric scaffoldKinaseProteinBiochemical modelPathwaySignal propagationTransductionComplexesQuantitative computer modelCrosstalkCompartmentsActivation