2022
SH3 domain regulation of RhoGAP activity: Crosstalk between p120RasGAP and DLC1 RhoGAP
Chau JE, Vish KJ, Boggon TJ, Stiegler AL. SH3 domain regulation of RhoGAP activity: Crosstalk between p120RasGAP and DLC1 RhoGAP. Nature Communications 2022, 13: 4788. PMID: 35970859, PMCID: PMC9378701, DOI: 10.1038/s41467-022-32541-4.Peer-Reviewed Original ResearchConceptsRhoGAP activitySH3 domainCatalytic arginine fingerIntrinsic GTPase activityRho family GTPasesLiver cancer 1GAP proteinsRhoGAP proteinArginine fingerCo-crystal structureRas GTPasesGAP activityRho proteinsCellular processesGTPase activityMolecular basisKey regulatorTumor suppressorP120RasGAPCell migrationProteinGTPasesRhoGAPCancer 1Binding sites
2020
The pseudoGTPase group of pseudoenzymes
Stiegler AL, Boggon TJ. The pseudoGTPase group of pseudoenzymes. The FEBS Journal 2020, 287: 4232-4245. PMID: 32893973, PMCID: PMC7544640, DOI: 10.1111/febs.15554.Peer-Reviewed Original ResearchConceptsSignal transductionAmino acid divergenceEnzymatic activityLeucine-rich repeat kinase 2Amino acid differencesP190RhoGAP proteinsRepeat kinase 2Membrane traffickingCellular rolesEnzyme foldImportant mechanistic componentSmall GTPasesTranscriptional controlCellular functionsGTPasesKinase 2PseudoenzymesAcid differencesEC numbersCell migrationFunctional roleMitochondrial activityProteinCargo transportMechanistic components
2018
PseudoGTPase domains in p190RhoGAP proteins: a mini-review.
Stiegler AL, Boggon TJ. PseudoGTPase domains in p190RhoGAP proteins: a mini-review. Biochemical Society Transactions 2018, 46: 1713-1720. PMID: 30514771, PMCID: PMC6501215, DOI: 10.1042/bst20180481.Peer-Reviewed Original ResearchConceptsP190RhoGAP proteinsDetectable catalytic activityAbundant GTPaseSmall GTPasesRho familyStructural biologyCatalytic cleftEnzyme classesNew family membersFamily membersNoncanonical residuesGTPase enzymeKey regulatorCell migrationOverall proteinProteinBiochemical analysisGTPaseRecent discoveryRecent studiesDomainPseudoenzymesGTPasesCytokinesisDiscovery