BD FACSAria in the Amistad building
This BD FACSAriaII is located in room 416 at Amistad building and is equipped with a biosafety cabinet, allowing to sort unscreened human material.
For more detailed information or to schedule a sort, please contact Ewa Menet at (203-785-2299).
UV Laser - 355 nm, 60 mW
PMT | Bandpass | Long Pass | Fluorochrome |
A | 740/35 | 690 LP | BUV737 |
B | 450/50 | 410 LP | AlexaFluor 350, DAPI |
C | 379/29 | BUV395 |
Violet Laser - 405 nm, 200 mW
PMT | Bandpass | Long Pass | Fluorochrome |
A | 710/50 | 685 LP | Qdot 705 |
B | 670/30 | 635 LP | Qdot 655 |
C | 610/20 | 600 LP | Qdot 605, BV605 |
D | 525/50 | 505 LP | AmCyan, Horizon V500 |
E | 450/50 | Pacific Blue, BV421, Horizon V450 |
Filters for BV786 are also available (780/60 and 755LP)
Blue Laser -488nm, 100 mW
PMT | Bandpass | Long Pass | Fluorochrome |
A | 682/33 | 635 LP | PerCP-Cy5.5 |
B | 515/20 | 505 LP | FITC, AlexaFluor488, CFSE, GFP,YFP |
C | 488/10 | SSC |
Green Laser - 532 nm, 150 mW
PMT | Bandpass | Long Pass | Fluorochrome |
A | 780/40 | 740 LP | PE-Cy7, PE-AF750 |
B | 710/50 | 685 LP | PE-Cy5.5 |
C | 660/40 | 640 LP | PE-Cy5, PE-AF647 |
D | 610/20 | 600 LP | AF594, PE-Texas Red, AF568, PI, Rhodamine-B |
E | 575/26 | PE, DsRed, RFP, Pyronin-Y, PKH26 |
Red Laser - 637nm, 140 mW
PMT | Bandpass | Long Pass | Fluorochrome |
A | 780/60 | 755 LP | APC-Cy7, Alexa Fluor 750, Alexa Fluor 780 |
B | 710/50 | 685 LP | APC-Cy5.5, Alexa Fluor 700 |
C | 660/20 | APC, Alexa Fluor 647 |