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BD FACSAria in the Amistad building

This BD FACSAriaII is located in room 416 at Amistad building and is equipped with a biosafety cabinet, allowing to sort unscreened human material.

For more detailed information or to schedule a sort, please contact Ewa Menet at (203-785-2299).

BD Biosciences web site

UV Laser - 355 nm, 60 mW

PMT Bandpass Long Pass Fluorochrome
A 740/35 690 LP BUV737
B 450/50 410 LP AlexaFluor 350, DAPI
C 379/29 BUV395

Violet Laser - 405 nm, 200 mW

PMT Bandpass Long Pass Fluorochrome
A 710/50 685 LP Qdot 705
B 670/30 635 LP Qdot 655
C 610/20 600 LP Qdot 605, BV605
D 525/50 505 LP AmCyan, Horizon V500
E 450/50 Pacific Blue, BV421, Horizon V450

Filters for BV786 are also available (780/60 and 755LP)

Blue Laser -488nm, 100 mW

PMT Bandpass Long Pass Fluorochrome
A 682/33 635 LP PerCP-Cy5.5
B 515/20 505 LP FITC, AlexaFluor488, CFSE, GFP,YFP
C 488/10 SSC

Green Laser - 532 nm, 150 mW

PMT Bandpass Long Pass Fluorochrome
A 780/40 740 LP PE-Cy7, PE-AF750
B 710/50 685 LP PE-Cy5.5
C 660/40 640 LP PE-Cy5, PE-AF647
D 610/20 600 LP AF594, PE-Texas Red, AF568, PI, Rhodamine-B
E 575/26 PE, DsRed, RFP, Pyronin-Y, PKH26

Red Laser - 637nm, 140 mW

PMT Bandpass Long Pass Fluorochrome
A 780/60 755 LP APC-Cy7, Alexa Fluor 750, Alexa Fluor 780
B 710/50 685 LP APC-Cy5.5, Alexa Fluor 700
C 660/20 APC, Alexa Fluor 647