2025
Solvent Transport in Disordered and Dynamic Membrane Pores: Implications for Reverse Osmosis and Nanofiltration Membranes
Fan H, Elimelech M. Solvent Transport in Disordered and Dynamic Membrane Pores: Implications for Reverse Osmosis and Nanofiltration Membranes. Environmental Science And Technology 2025, 59: 17922-17931. PMID: 40815600, PMCID: PMC12392456, DOI: 10.1021/acs.est.5c07128.Peer-Reviewed Original ResearchConceptsReverse osmosisNanofiltration membranesSolvent permeabilityPore sizeSolvent transportNanoporous membranesSolvent transport mechanismsLocal friction coefficientInfluence of pore connectivityMembrane poresMembrane separation technologyImpact of pore structurePressure-driven separationViscous naturePorous structure propertiesPore connectivityMembrane performanceFriction coefficientMolecular dynamics simulationsMembrane porosityPorous structurePolymeric membranesNanofiltrationPore structureSolvent moleculesCompaction of Pressure-Driven Polymer Membranes: Measurements, Theory, and Mechanisms
Fan H, Duan Y, Elimelech M. Compaction of Pressure-Driven Polymer Membranes: Measurements, Theory, and Mechanisms. Environmental Science And Technology 2025, 59: 14752-14763. PMID: 40623242, DOI: 10.1021/acs.est.5c05474.Peer-Reviewed Original ResearchConceptsStress-strain behaviorPore sizePermeation testsPermeate sideMembrane compactionPressure-driven membrane processesMembrane permeate sideDesign of membranesDead-end cellSolvent-swollen filmsCompaction of poresCompaction behaviorCompact porosityPorous filmsFeed sideMembrane porosityPorous structurePolymeric membranesPorosity gradientSolvent permeabilityCompression pressureMembrane processesSeparation performanceMaterial selectionSolvent transportRole of Transmembrane Pressure and Water Flux in Reverse Osmosis Composite Membrane Compaction and Performance
Wu J, He J, Quezada-Renteria J, Xiao M, Le J, Au K, Guo K, Ye N, Toma T, Elimelech M, Li Y, Hoek E. Role of Transmembrane Pressure and Water Flux in Reverse Osmosis Composite Membrane Compaction and Performance. Environmental Science And Technology 2025, 59: 8856-8866. PMID: 40267220, DOI: 10.1021/acs.est.5c02618.Peer-Reviewed Original ResearchMeSH KeywordsMembranes, ArtificialMolecular Dynamics SimulationOsmosisPermeabilityPorosityPressureWaterConceptsPolysulfone support layerTransmembrane pressureMembrane compactionSupport layerScanning electron microscopeWater fluxActive layerThin-film composite reverse osmosisConstant feed pressurePA selective layerTFC RO membraneComposite reverse osmosisPA active layerRO membrane performanceHigh water fluxHydraulic pressure dropLow transmembrane pressureTFC-RORO membranesSalt rejectionMembrane performanceCompaction behaviorReverse osmosisSelective layerFeed pressureRelating Solute–Membrane Electrostatic Interactions to Solute Permeability in Reverse Osmosis Membranes
Cao T, Wang L, Pataroque K, Wang R, Elimelech M. Relating Solute–Membrane Electrostatic Interactions to Solute Permeability in Reverse Osmosis Membranes. Environmental Science And Technology 2025, 59: 5819-5828. PMID: 40070342, DOI: 10.1021/acs.est.4c13212.Peer-Reviewed Original ResearchConceptsRO membranesCellulose triacetateSalt rejectionPA membraneReverse osmosisElectrostatic interactionsSolution pHReverse osmosis membranesDonnan potentialSolute transportSolute permeabilityNeutral moleculesOsmosis membranesCellulose triacetate membraneHigher solution pHSalt permeabilityMembrane-solution interfaceWater desalinationCo ionsSolute-membrane interactionsMembrane charge densityAcidic conditionsCharge densityNeutral solute transportDonnan effect
2024
Channel width modulates the permeability of DNA origami–based nuclear pore mimics
Feng Q, Saladin M, Wu C, Cao E, Zheng W, Zhang A, Bhardwaj P, Li X, Shen Q, Kapinos L, Kozai T, Mariappan M, Lusk C, Xiong Y, Lim R, Lin C. Channel width modulates the permeability of DNA origami–based nuclear pore mimics. Science Advances 2024, 10: eadq8773. PMID: 39536094, PMCID: PMC11559598, DOI: 10.1126/sciadv.adq8773.Peer-Reviewed Original Research
2023
Disturbed flow increases endothelial inflammation and permeability via a Frizzled-4-β-catenin-dependent pathway
Rickman M, Ghim M, Pang K, von Huelsen Rocha A, Drudi E, Sureda-Vives M, Ayoub N, Tajadura-Ortega V, George S, Weinberg P, Warboys C. Disturbed flow increases endothelial inflammation and permeability via a Frizzled-4-β-catenin-dependent pathway. Journal Of Cell Science 2023, 136: jcs260449. PMID: 36846872, PMCID: PMC10112981, DOI: 10.1242/jcs.260449.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsbeta CateninEndothelial CellsFrizzled ReceptorsHumansInflammationPermeabilitySwineWnt Signaling PathwayConceptsEndothelial dysfunctionEndothelial cellsFrizzled-4Β-cateninCultured human aortic endothelial cellsR-spondinsHuman aortic endothelial cellsPro-inflammatory genesEndothelial paracellular permeabilityDisturbed flowAortic endothelial cellsCultured endothelial cellsRole of WntEndothelial inflammationAortic archEarly atherogenesisCanonical Wnt pathwayDysfunctionReduced expressionWnt5a signalingParacellular permeabilityWnt pathwayInhibitionKnockdownExpression
2022
Radiochemical Synthesis and Evaluation of 3‑[11C]Methyl-4-aminopyridine in Rodents and Nonhuman Primates for Imaging Potassium Channels in the CNS
Sun Y, Guehl N, Zhou Y, Takahashi K, Belov V, Dhaynaut M, Moon S, Fakhri G, Normandin M, Brugarolas P. Radiochemical Synthesis and Evaluation of 3‑[11C]Methyl-4-aminopyridine in Rodents and Nonhuman Primates for Imaging Potassium Channels in the CNS. ACS Chemical Neuroscience 2022, 13: 3342-3351. PMID: 36417797, PMCID: PMC9732819, DOI: 10.1021/acschemneuro.2c00364.Peer-Reviewed Original ResearchConceptsRegional brain time-activity curvesBrain time-activity curvesOne-tissue compartment modelBlood-brain barrierConsistent with <i>inNonhuman primatesTime-activity curvesPositron emission tomographySpinal cord injuryDemyelinated neuronsNeurological symptomsPotassium channelsNeuronal conductionBrain uptakeEmission tomographyStille cross-couplingMultiple sclerosisCord injurySlow kineticsBrain permeabilityTraumatic brainMyelin sheathStronger binding affinityNeuronsBrainNO Synthesis but Not Apoptosis, Mitosis or Inflammation Can Explain Correlations between Flow Directionality and Paracellular Permeability of Cultured Endothelium
Ghim M, Yang S, David K, Eustaquio J, Warboys C, Weinberg P. NO Synthesis but Not Apoptosis, Mitosis or Inflammation Can Explain Correlations between Flow Directionality and Paracellular Permeability of Cultured Endothelium. International Journal Of Molecular Sciences 2022, 23: 8076. PMID: 35897652, PMCID: PMC9332325, DOI: 10.3390/ijms23158076.Peer-Reviewed Original Research
2021
Oral anaphylaxis to peanut in a mouse model is associated with gut permeability but not with Tlr4 or Dock8 mutations
Gertie JA, Zhang B, Liu EG, Hoyt LR, Yin X, Xu L, Long LL, Soldatenko A, Gowthaman U, Williams A, Eisenbarth SC. Oral anaphylaxis to peanut in a mouse model is associated with gut permeability but not with Tlr4 or Dock8 mutations. Journal Of Allergy And Clinical Immunology 2021, 149: 262-274. PMID: 34051223, PMCID: PMC8626534, DOI: 10.1016/j.jaci.2021.05.015.Peer-Reviewed Original ResearchMeSH KeywordsAdministration, OralAnimalsArachisDisease Models, AnimalFemaleGastrointestinal MicrobiomeGenetic Predisposition to DiseaseGuanine Nucleotide Exchange FactorsIntestinal MucosaMaleMice, Inbred C3HMice, Inbred C57BLMutationPassive Cutaneous AnaphylaxisPeanut HypersensitivityPermeabilitySpecies SpecificityToll-Like Receptor 4ConceptsC3H/HeJ miceGut permeabilityHeJ miceOral anaphylaxisPeanut challengeFood allergyMouse modelToll-like receptor 4BALB/c miceCytokinesis 8 (DOCK8) mutationsEnhanced gut permeabilityOral peanut challengesDOCK8 functionIngested allergenImmunologic mechanismsAnaphylaxis responsesImmunologic pathwaysAllergic diseasesC57BL/6 micePeanut allergyAntibody responseC miceReceptor 4DOCK8 mutationsIntraperitoneal challengeA venous-specific purinergic signaling cascade initiated by Pannexin 1 regulates TNFα-induced increases in endothelial permeability
Maier-Begandt D, Comstra H, Molina S, Krüger N, Ruddiman C, Chen Y, Chen X, Biwer L, Johnstone S, Lohman A, Good M, DeLalio L, Hong K, Bacon H, Yan Z, Sonkusare S, Koval M, Isakson B. A venous-specific purinergic signaling cascade initiated by Pannexin 1 regulates TNFα-induced increases in endothelial permeability. Science Signaling 2021, 14 PMID: 33653920, PMCID: PMC8011850, DOI: 10.1126/scisignal.aba2940.Peer-Reviewed Original ResearchConceptsRNA expression profilingHydrolysis of ATPPannexin-1 channelsExpression profilingArterial endothelial cellsEndothelial cellsArterial endotheliumVenous endothelial cellsBarrier functionProtein analysisLung vascular permeabilityPannexin-1Claudin compositionBarrier function impairmentProinflammatory cytokine TNFαEndothelial cell barrierTargetable pathwaysMurine veinCecal ligationCell barrierFunction impairmentInflammatory insultLife spanDeficient miceVascular leakPreclinical Comparison of the Blood–brain barrier Permeability of Osimertinib with Other EGFR TKIs
Colclough N, Chen K, Johnström P, Strittmatter N, Yan Y, Wrigley GL, Schou M, Goodwin R, Varnäs K, Adua SJ, Zhao M, Nguyen DX, Maglennon G, Barton P, Atkinson J, Zhang L, Janefeldt A, Wilson J, Smith A, Takano A, Arakawa R, Kondrashov M, Malmquist J, Revunov E, Vazquez-Romero A, Moein MM, Windhorst AD, Karp NA, Finlay MRV, Ward RA, Yates JWT, Smith PD, Farde L, Cheng Z, Cross DAE. Preclinical Comparison of the Blood–brain barrier Permeability of Osimertinib with Other EGFR TKIs. Clinical Cancer Research 2021, 27: 189-201. PMID: 33028591, DOI: 10.1158/1078-0432.ccr-19-1871.Peer-Reviewed Original ResearchConceptsBlood-brain barrier permeabilityBrain metastasesBrain penetranceBarrier permeabilityEGFR tyrosine kinase inhibitorsT790M resistance mutationMetastatic brain diseaseSubclinical brain metastasesSelective EGFR tyrosine kinase inhibitorOngoing clinical evaluationM resistance mutationTyrosine kinase inhibitorsBBB penetranceBrain tumor growthClinical efficacyEGFR-TKIEGFR-TKIsBrain penetrationClinical evaluationPreclinical comparisonPreclinical modelsPreclinical studiesCynomolgus macaquesOsimertinibTumor growth
2020
The Role of Tricellular Junctions in the Transport of Macromolecules Across Endothelium
Ghim M, Mohamied Y, Weinberg P. The Role of Tricellular Junctions in the Transport of Macromolecules Across Endothelium. Cardiovascular Engineering And Technology 2020, 12: 101-113. PMID: 32820467, PMCID: PMC7904563, DOI: 10.1007/s13239-020-00483-x.Peer-Reviewed Original ResearchConceptsDensity lipoproteinHigh-density lipoproteinLow-density lipoproteinNew therapeutic strategiesEffects of agonistsEndothelial barrier functionConclusionThese dataInfluence of agonistsTherapeutic strategiesVascular endotheliumEndotheliumBarrier functionEndothelial monolayersNormal physiologyTransendothelial transportAgonistsLipoproteinDiseaseFurther investigationATP synthase c-subunit ring as the channel of mitochondrial permeability transition: Regulator of metabolism in development and degeneration
Mnatsakanyan N, Jonas EA. ATP synthase c-subunit ring as the channel of mitochondrial permeability transition: Regulator of metabolism in development and degeneration. Journal Of Molecular And Cellular Cardiology 2020, 144: 109-118. PMID: 32461058, PMCID: PMC7877492, DOI: 10.1016/j.yjmcc.2020.05.013.Peer-Reviewed Original ResearchConceptsMitochondrial permeability transition poreC subunit ringMitochondrial permeability transitionPermeability transitionRegulator of metabolismPermeability transition poreImportant metabolic regulatorMitochondrial megachannelBiology todayRegulatory mechanismsCentral playerTransition poreMetabolic regulatorMolecular compositionRecent findingsRegulatorDegenerative diseasesPathophysiological roleRecent advancesMegachannelRoleMetabolismMysterious phenomenonBMX Represses Thrombin-PAR1–Mediated Endothelial Permeability and Vascular Leakage During Early Sepsis
Li Z, Yin M, Zhang H, Ni W, Pierce R, Zhou HJ, Min W. BMX Represses Thrombin-PAR1–Mediated Endothelial Permeability and Vascular Leakage During Early Sepsis. Circulation Research 2020, 126: 471-485. PMID: 31910739, PMCID: PMC7035171, DOI: 10.1161/circresaha.119.315769.Peer-Reviewed Original ResearchConceptsPAR1 internalizationPuncture-induced sepsisCecal ligationVascular leakageEndothelial permeabilityExpression of BmxThrombin-PAR1Early sepsisEndothelial cellsPuncture modelSignal inactivationPAR1 antagonist SCH79797Negative regulatorLung epithelial cellsTransendothelial electrical resistanceAdult stageEmbryonic stagesCultured endothelial cellsPulmonary leakageCellular analysisLung injuryPathological stimuliEndothelium dysfunctionPlatelet dysfunctionSepsis
2018
Methods to Probe Calcium Regulation by BCL-2 Family Members
Carpio MA, Katz SG. Methods to Probe Calcium Regulation by BCL-2 Family Members. Methods In Molecular Biology 2018, 1877: 173-183. PMID: 30536006, DOI: 10.1007/978-1-4939-8861-7_12.Peer-Reviewed Original ResearchConceptsMitochondrial outer membrane permeabilizationBcl-2 family membersEndoplasmic reticulumOuter membrane permeabilizationProcess of apoptosisSuch important functionsMembrane permeabilizationDirect regulationFamily membersAdditional roleImportant functionsCalcium regulationApoptosisRegulationRelease of calciumMitochondriaPermeabilizationBiologyCytosolMembersReticulumTo BBB or Not to BBB?
Thomas JL, Eichmann A. To BBB or Not to BBB? Developmental Cell 2018, 47: 689-691. PMID: 30562511, DOI: 10.1016/j.devcel.2018.11.039.Peer-Reviewed Original ResearchAssessing the role of residue E73 and lipid headgroup charge in VDAC1 voltage gating
Queralt-Martín M, Bergdoll L, Jacobs D, Bezrukov S, Abramson J, Rostovtseva T. Assessing the role of residue E73 and lipid headgroup charge in VDAC1 voltage gating. Biochimica Et Biophysica Acta (BBA) - Bioenergetics 2018, 1860: 22-29. PMID: 30412693, PMCID: PMC8283775, DOI: 10.1016/j.bbabio.2018.11.001.Peer-Reviewed Original ResearchConceptsVoltage-dependent anion channelMitochondrial outer membraneVoltage gatingVoltage-gating processCholesterol binding siteVDAC's roleVDAC functionGating processMetabolite transportOuter membraneAbundant proteinsMOM permeabilityVDAC gatingPlanar lipid membranesAnion channelE73Mitochondrial respirationLipid bilayer systemsLipid headgroup chargeBinding sitesLipid membranesPhospholipid headgroupsElectrophysiology measurementsTransport of ionsRecent studiesA Programmable DNA Origami Platform for Organizing Intrinsically Disordered Nucleoporins within Nanopore Confinement
Fisher PDE, Shen Q, Akpinar B, Davis LK, Chung KKH, Baddeley D, Šarić A, Melia TJ, Hoogenboom BW, Lin C, Lusk CP. A Programmable DNA Origami Platform for Organizing Intrinsically Disordered Nucleoporins within Nanopore Confinement. ACS Nano 2018, 12: 1508-1518. PMID: 29350911, PMCID: PMC5834394, DOI: 10.1021/acsnano.7b08044.Peer-Reviewed Original ResearchConceptsTransport channelsAtomic force microscopyMolecular dynamics simulationsHigh-speed atomic force microscopyDNA origami platformFG domainsNuclear pore complexes (NPCs) formChannel mimicsCentral transport channelNuclear pore proteinsForce microscopyDNA origamiNuclear transport receptorsDynamics simulationsSelective transportNanopore confinementMolecular exchangePermeability propertiesDNA cylindersChemical compositionFG networkPore proteinsPolymer modelTransport receptorsCollective propertiesER-stress mobilization of death-associated protein kinase-1–dependent xenophagy counteracts mitochondria stress–induced epithelial barrier dysfunction
Lopes F, Keita Å, Saxena A, Reyes J, Mancini N, Al Rajabi A, Wang A, Baggio C, Dicay M, van Dalen R, Ahn Y, Carneiro M, Peters N, Rho J, MacNaughton W, Girardin S, Jijon H, Philpott D, Söderholm J, McKay D. ER-stress mobilization of death-associated protein kinase-1–dependent xenophagy counteracts mitochondria stress–induced epithelial barrier dysfunction. Journal Of Biological Chemistry 2018, 293: 3073-3087. PMID: 29317503, PMCID: PMC5836131, DOI: 10.1074/jbc.ra117.000809.Peer-Reviewed Original ResearchConceptsDeath-associated protein kinase 1ER stressEndoplasmic reticulumAutophagy gene <i>ATG16L1</i> isEpithelial barrier functionInduction of ER stressDisruption of mitochondrial functionER stressor tunicamycinInflammatory bowel diseaseUptake of bacteriaDNP-treated miceUncoupling of oxidative phosphorylationProtein kinase 1Dextran sodium sulfateStressor tunicamycinBarrier functionTranslocation of commensal bacteriaDysfunctional mitochondriaGut microbiomeMitochondrial stressEpithelial mitochondriaCommensal bacteriaOxidative phosphorylationATF6 armMitochondrial function
2016
The cystic fibrosis transmembrane conductance regulator controls biliary epithelial inflammation and permeability by regulating Src tyrosine kinase activity
Fiorotto R, Villani A, Kourtidis A, Scirpo R, Amenduni M, Geibel PJ, Cadamuro M, Spirli C, Anastasiadis PZ, Strazzabosco M. The cystic fibrosis transmembrane conductance regulator controls biliary epithelial inflammation and permeability by regulating Src tyrosine kinase activity. Hepatology 2016, 64: 2118-2134. PMID: 27629435, PMCID: PMC5115965, DOI: 10.1002/hep.28817.Peer-Reviewed Original ResearchConceptsBiliary epithelial cellsLiver diseaseToll-like receptor 4 activityToll-like receptor 4 responsesCystic fibrosis transmembrane conductance regulatorToll-like receptor 4Nuclear factorEpithelial cellsProinflammatory cytokine productionNovel therapeutic targetEpithelial barrier functionActivated B cellsFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorCytokine productionEpithelial inflammationInflammatory cellsInflammatory processReceptor 4Biliary damageInflammatory responseInflammatory cholangiopathyProtective effectBile secretionImmune pathways
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