2025
Proteoglycan-4 (PRG4) serum concentration is lower in aged mice, and genetic deficiency impacts survival probability, blood parameters, and bone during aging
Tanguay A, Menon N, Slavin E, Moody M, McEwen E, Jay G, Lorenzo J, Bartley J, Scanlon V, Deymier A, Schmidt T. Proteoglycan-4 (PRG4) serum concentration is lower in aged mice, and genetic deficiency impacts survival probability, blood parameters, and bone during aging. GeroScience 2025, 1-14. PMID: 40537697, DOI: 10.1007/s11357-025-01747-x.Peer-Reviewed Original ResearchWT miceCamptodactyly-arthropathy-coxa vara-pericarditisProteoglycan 4Aged wild typeYoung WT miceComplete blood countLoss-of-function mutationsAssessed clinical relevanceProteoglycan 4 geneBlood countAged miceSerum concentrationsAnti-fibroticRare diseaseBlood gasesClinical relevanceImmunomodulatory propertiesMiceWild typeBlood parametersYounger ageSurvival probabilitySex-dependentSerumLower survival probabilityEPS4.09The gut's role in pulmonary and extrapulmonary manifestation of cystic fibrosis
Cigana C, Delmonte G, Faccoetti A, Veschetti L, Gianferro F, Neri A, Fiorotto R, Livraghi-Butrico A, Massimino L, Ungaro F, Bragonzi A. EPS4.09The gut's role in pulmonary and extrapulmonary manifestation of cystic fibrosis. Journal Of Cystic Fibrosis 2025, 24: s52. DOI: 10.1016/j.jcf.2025.03.626.Peer-Reviewed Original ResearchWT miceCystic fibrosisHO miceDistal organsCollaborative CrossGenetically diverse Collaborative CrossInflammatory responseBarrier integrityManifestations of cystic fibrosisMouse models of CFEnteric bacteriaIncreased gut permeabilityCFTR modulator treatmentModel of CFEnhanced defense responsesGut barrier integrityImmune response pathwaysHigher bacterial loadsExtrapulmonary manifestationsSystemic inflammationChronic infectionInflammatory profileGut permeabilityGenomic toolsMicrobiological cultureMechanism of Impaired Potassium Transport in Pseudohypoaldosteronism
Wan L, Lessa L, Shen H, Malnic G, Giebisch G, Wang T. Mechanism of Impaired Potassium Transport in Pseudohypoaldosteronism. Physiology 2025, 40: 0956. DOI: 10.1152/physiol.2025.40.s1.0956.Peer-Reviewed Original ResearchMaxi-K channelsK+ secretionMaxi-KPseudohypoaldosteronism type IILate distal tubuleDistal tubulesHK intakeInhibitor iberiotoxinNaCl cotransporterDistal nephronNaCl reabsorptionConductance Ca2+-activated K+Thiazide-sensitive NaCl cotransporterCa2+-activated K+Renal outer medullary potassiumCortical distal tubulesRenal NaCl reabsorptionIncreased K+ secretionReduced NaCl reabsorptionWNK4 mutationsDouble-barreled microelectrodesChannel-mediated mechanismsNCC expression-activated K+WT miceSustained tenascin-C expression drives neointimal hyperplasia and promotes aortocaval fistula failure
Gonzalez L, Zhang W, Bai H, Taniguchi R, Ramachandra A, Jovin D, Ohashi Y, Nguyen M, Thaxton C, Yatsula B, Vazquez-Padron R, Humphrey J, Martin K, Kyriakides T, Dardik A. Sustained tenascin-C expression drives neointimal hyperplasia and promotes aortocaval fistula failure. AJP Heart And Circulatory Physiology 2025, 328: h1147-h1167. PMID: 40247455, PMCID: PMC12150301, DOI: 10.1152/ajpheart.00661.2024.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArteriovenous Shunt, SurgicalDisease Models, AnimalGraft Occlusion, VascularHuman Umbilical Vein Endothelial CellsHumansHyperplasiaMacrophagesMaleMiceMice, Inbred C57BLMice, KnockoutMyocytes, Smooth MuscleNeointimaNF-kappa BTenascinThrombomodulinVascular PatencyVascular RemodelingVena Cava, InferiorConceptsArteriovenous fistula patencyTenascin-C expressionArteriovenous fistulaTissue factor expressionEnd-stage kidney diseaseAnti-inflammatory macrophage polarizationNeointimal hyperplasiaWT miceTenascin-CInflammatory microenvironmentWild-typeFactor expressionMacrophage polarizationArteriovenous fistula outcomesArteriovenous fistula failurePatent arteriovenous fistulaVein smooth muscle cellsArteriovenous fistula maturationSmooth muscle cellsOccluded arteriovenous fistulaHuman umbilical vein smooth muscle cellsImprove dialysis outcomesHuman umbilical vein endothelial cellsRegulation of inflammationNF-kB activationP112 Cytokine driven disease and epigenetic heterogeneity in rheumatoid arthritis
Hughes S, Costa D, Soria A, Hill D, Figueras A, Scott R, Dimonte S, Monaco F, Jenkins R, Twohig J, Guy C, Cossins B, Andrews R, Szomolay B, Choy E, Vinh N, Lewis M, Jenkins B, Turner S, Tiganis T, Williams N, Yu H, Pitzalis C, Jones G, Jones S. P112 Cytokine driven disease and epigenetic heterogeneity in rheumatoid arthritis. Rheumatology 2025, 64: keaf142.152. DOI: 10.1093/rheumatology/keaf142.152.Peer-Reviewed Original ResearchEctopic lymphoid-like structuresAntigen-induced arthritisRNA-seqTransposase-accessible chromatin (ATAC)-seqRheumatoid arthritisChromatin immunoprecipitation (ChIP)-seqTreatment of immune-mediated inflammatory diseasesClinical response to therapyCytokine signalingSynovial biopsiesImmune-mediated inflammatory diseasesSmall-needle biopsiesLymphoid-like structuresResponse to therapyNature of pathologyWT miceEpigenetic heterogeneityNeedle biopsyTargeting cytokine signallingGenomic signaturesImmune pathologyNGS methodSTAT3 activationTranscription factorsEpigenetic regulation
2024
Identification of FGFR4 as a regulator of myofibroblast differentiation in pulmonary fibrosis
Ghanem M, Justet A, Jaillet M, Vasarmidi E, Boghanim T, Hachem M, Vadel A, Joannes A, Mordant P, Balayev A, Adams T, Mal H, Cazes A, Poté N, Mailleux A, Crestani B. Identification of FGFR4 as a regulator of myofibroblast differentiation in pulmonary fibrosis. American Journal Of Physiology - Lung Cellular And Molecular Physiology 2024, 327: l818-l830. PMID: 39350729, DOI: 10.1152/ajplung.00184.2023.Peer-Reviewed Original ResearchWild type littermatesFibroblast growth factorMurine embryonic fibroblastsEndothelin-1Pulmonary fibrosisFGFR4 inhibitionBleomycin-Induced Pulmonary FibrosisIn vitroMyofibroblast differentiationBleomycin-induced lung fibrosisPulmonary fibrosis in vivoTGF-bLung fibroblastsPro-fibrotic propertiesProtein levelsIn vivoAnti-fibrotic propertiesFibrosis in vivoRegulation of myofibroblast differentiationDevelopment of bleomycin-induced lung fibrosisWT miceTherapeutic optionsHuman lung fibroblastsIPF lungsLung fibrosisCYP2E1 in 1,4-dioxane metabolism and liver toxicity: insights from CYP2E1 knockout mice study
Wang Y, Charkoftaki G, Orlicky D, Davidson E, Aalizadeh R, Sun N, Ginsberg G, Thompson D, Vasiliou V, Chen Y. CYP2E1 in 1,4-dioxane metabolism and liver toxicity: insights from CYP2E1 knockout mice study. Archives Of Toxicology 2024, 98: 3241-3257. PMID: 39192018, PMCID: PMC11500436, DOI: 10.1007/s00204-024-03811-5.Peer-Reviewed Original ResearchCYP2E1-null miceLiver toxicityDrinking waterOxidative DNA damageLiver carcinogenAbstract1,4-DioxaneDNA damage repair responseImpaired DNA damage repairWater contaminationOxidative stressElevated oxidative stressEnvironmental pollutionKnockout mouse studiesDamage repair responseCYP2E1-nullMale wildtypeWT miceDNA damageDX exposureRisk assessmentRedox dysregulationCYP2E1 inductionLiver oxidative stressHigh dosesMouse studiesSmall molecule inhibition of glycogen synthase I reduces muscle glycogen content and improves biomarkers in a mouse model of Pompe disease
Gaspar R, Sakuma I, Nasiri A, Hubbard B, LaMoia T, Leitner B, Tep S, Xi Y, Green E, Ullman J, Petersen K, Shulman G. Small molecule inhibition of glycogen synthase I reduces muscle glycogen content and improves biomarkers in a mouse model of Pompe disease. AJP Endocrinology And Metabolism 2024, 327: e524-e532. PMID: 39171753, PMCID: PMC11482269, DOI: 10.1152/ajpendo.00175.2024.Peer-Reviewed Original ResearchGAA-KO miceMouse model of Pompe diseaseModel of Pompe diseasePompe diseaseMetabolic dysregulationRegular chowMouse modelSmall molecule inhibitionInsulin sensitivityReduced spontaneous activityGroups of male miceEnzyme acid alpha-glucosidaseProgressive muscle weaknessImprove metabolic dysregulationSynthase IWhole-body insulin sensitivityAcid alpha-glucosidaseImproved glucose toleranceIncreased AMPK phosphorylationWT miceAbnormal accumulation of glycogenGlycogen storage disorderMale miceSpontaneous activityImproved biomarkersGlobal deletion of G protein‐coupled receptor 55 impairs glucose homeostasis during obesity by reducing insulin secretion and β‐cell turnover
Liu B, Ruz‐Maldonado I, Persaud S. Global deletion of G protein‐coupled receptor 55 impairs glucose homeostasis during obesity by reducing insulin secretion and β‐cell turnover. Diabetes Obesity And Metabolism 2024, 26: 4591-4601. PMID: 39113250, DOI: 10.1111/dom.15816.Peer-Reviewed Original ResearchGPR55<sup>-/-</sup> miceDiet-induced obesityIncreased islet cell apoptosisHigh-fat dietB cell proliferationWild-typeCytokine-induced apoptosisInsulin secretionCaspase-3/7 activityIslet cell apoptosisGlucose homeostasisInsulin secretion in vivoWT miceB cellsDynamic insulin secretionImpaired glucose-Standard chowSusceptibility to diet-induced obesityPerifusion of isolated isletsIslet functionG protein-coupled receptorsG protein-coupled receptor 55Weeks of dietary interventionReduced insulin secretionB cell turnover1574-P: Catecholamine-Induced Adipose Lipolysis Drives Hepatic Steatosis in Pnpla3I148M Knockin Mice
PRAKASH GOLLA J, SUH R, PAOLELLA L, STROBER J, ZHANG F, PHILBRICK W, VATNER D. 1574-P: Catecholamine-Induced Adipose Lipolysis Drives Hepatic Steatosis in Pnpla3I148M Knockin Mice. Diabetes 2024, 73 DOI: 10.2337/db24-1574-p.Peer-Reviewed Original ResearchWhite adipose tissueM miceDecreased hepatic triglycerideHepatic steatosisMutant miceCatecholamine responseGonadal white adipose tissueBeta-adrenergic blockadeHepatic triglycerideWhite adipose tissue functionResponse to CLHigh-sucrose dietHepatic triglyceride contentWT miceM mutationExtrahepatic sourcesAdrenergic blockadeHepatic triglyceride biosynthesisAgonist CLEx vivoInsulin responseI148MMiceFatty liverAdipose tissueD239Y germline variant of NTHL1 glycosylase increases susceptibility to colorectal cancer in mice
Masannat T, Ghishan F, Kiela P, Sweasy J. D239Y germline variant of NTHL1 glycosylase increases susceptibility to colorectal cancer in mice. Physiology 2024, 39: 2118. DOI: 10.1152/physiol.2024.39.s1.2118.Peer-Reviewed Original ResearchInflammation-associated colorectal cancerColorectal cancerDistal colonColon lengthSusceptibility to colorectal cancerC57BL/6 wild-typeMicronucleated normochromatic erythrocytesProximal tumor locationFemale C57BL/6 wild-typeIncreased lifetime riskDextran sulfate sodiumSize of polypsMonitoring body weightKnock-In MiceIncreased polyp numberDominant-negative mutationsNormal drinking waterDSS cycleTumor locationWT miceNormochromatic erythrocytesDNA damageWT littermatesColitis scoresRepair mechanismsEzrin drives adaptation of monocytes to the inflamed lung microenvironment.
Gudneppanavar R, Di Pietro C, Oez H, Zhang P, Huang P, Braga C, Tebaldi T, Biancon G, Kim C, Gonzalez A, Halene S, Krause D, Egan M, Gupta N, Murray T, Bruscia E. Ezrin drives adaptation of monocytes to the inflamed lung microenvironment. The Journal Of Immunology 2024, 212: 0078_5418-0078_5418. DOI: 10.4049/jimmunol.212.supp.0078.5418.Peer-Reviewed Original ResearchRNA-seqActin-binding protein ezrinF-actin distributionImmune response to bacteriaCystic fibrosisIn vitro functional studiesResponse to bacteriaIncreased expression of pro-inflammatory markersCytoskeleton rearrangementF-actinResponse to lung infectionExpressed genesProtein ezrinTranscriptional profilesExpression of pro-inflammatory markersPlasma membranePro-inflammatory markersFunctional studiesEzrinLung extracellular matrixCF miceExtracellular matrixWT micePI3K/Akt signalingLung infectionImpact of Abl2/Arg deficiency on anxiety and depressive behaviors in mice
Yao X, Chen R, Chen H, Koleske A, Xiao X. Impact of Abl2/Arg deficiency on anxiety and depressive behaviors in mice. Behavioural Brain Research 2024, 468: 115022. PMID: 38697301, DOI: 10.1016/j.bbr.2024.115022.Peer-Reviewed Original ResearchDepressive-like behaviorBehavioral testsMarble-burying behavior testAnxiety/depression-related behaviorsAnxiety-like behaviorElevated plus mazeTail suspension testEmotion-related behaviorsOpen field testWT micePlus mazeDepressive behaviorSuspension testAgonist gaboxadolMemory deficitsY-mazeAnxietyInhibitory neurotransmissionCompared to WT miceSensory/motor functionTissue injury responsesWild-typeHippocampusGABAergic synapseIntraperitoneal treatment
2023
Dual PET imaging of microtubules and synaptic density in a mouse model of Alzheimer’s Disease
Damuka N, Bansode A, Krizan I, Gollepalli K, Miller M, Bhoopal B, Whitlow C, Lockhart S, Craft S, Sai K. Dual PET imaging of microtubules and synaptic density in a mouse model of Alzheimer’s Disease. Alzheimer's & Dementia 2023, 19 DOI: 10.1002/alz.078865.Peer-Reviewed Original ResearchStandardized uptake valueWT miceMT destabilizationWhole-brain standardized uptake valuesMouse modelBrain radioactivity uptakeSV2A levelsBrain uptakeStandardized uptake value analysisEx vivo biodistributionIn vitro autoradiography studiesIn vivo PETSynaptic lossAPP/PS1 miceSynaptic vesicle proteinsBrain PET/CTPET-CTTg-APP/PS1 miceWT littermatesMouse model of Alzheimer's diseaseUptake valueWestern blot analysisPET evaluationAutoradiography dataRadioactivity uptakePET Imaging of Rho‐Associated Protein Kinase 2 in A Mouse Model of Alzheimer’s Disease
Zheng C, Nicholson L, Chen B, Toyonaga T, Liu M, Strittmatter S, Carson R, Huang Y, Cai Z. PET Imaging of Rho‐Associated Protein Kinase 2 in A Mouse Model of Alzheimer’s Disease. Alzheimer's & Dementia 2023, 19 DOI: 10.1002/alz.081695.Peer-Reviewed Original ResearchAPP/PS1 micePS1 miceWT miceCentral nervous systemTime-activity curvesAlzheimer's diseaseAPP/PS1 transgenic miceAPP/PS1 transgenic AD miceMouse brainAge-matched WT controlsPS1 transgenic miceAPP/PS1Transgenic AD miceDynamic PET imaging dataROCK2 protein expressionAD drug discoveryHigh tracer uptakeMin post injectionPET imaging resultsExpression levelsReference tissue model 2PET imaging dataProtein expression levelsAD miceRegional time-activity curvesDual PET imaging of microtubules and synaptic density in a mouse model of Alzheimer’s Disease
Damuka N, Bansode A, Krizan I, Gollepalli K, Miller M, Bhoopal B, Whitlow C, Lockhart S, Craft S, Sai K. Dual PET imaging of microtubules and synaptic density in a mouse model of Alzheimer’s Disease. Alzheimer's & Dementia 2023, 19 DOI: 10.1002/alz.081849.Peer-Reviewed Original ResearchStandardized uptake valueWT miceWhole-brain standardized uptake valuesMouse modelBrain radioactivity uptakeBrain uptakeSV2A levelsStandardized uptake value analysisMT destabilizationEx vivo biodistributionIn vitro autoradiography studiesIn vivo PETBrain PET/CTPET-CTWT littermatesUptake valueWestern blot analysisPET evaluationSynaptic lossAutoradiography dataRadioactivity uptakeAPP/PS1 miceMicroPET/CT imagingAbstract BackgroundIn vivo levelsSodium currents in naïve mouse dorsal root ganglion neurons: No major differences between sexes
Ghovanloo M, Tyagi S, Zhao P, Effraim P, Dib-Hajj S, Waxman S. Sodium currents in naïve mouse dorsal root ganglion neurons: No major differences between sexes. Channels 2023, 18: 2289256. PMID: 38055732, PMCID: PMC10761158, DOI: 10.1080/19336950.2023.2289256.Peer-Reviewed Original ResearchConceptsSexual dimorphismRodent dorsal root ganglion neuronsBiophysical propertiesDorsal root ganglion neuronsExpression patternsSex-dependent regulationVoltage-gated sodiumFunctional analysisGanglion neuronsRodent sensory neuronsMouse dorsal root ganglion neuronsNaïve WT miceNumber of cellsMixed populationDimorphismUniform experimental conditionsSex-dependent differencesSensory neuronsNative DRG neuronsPain pathwaysDRG neuronsWT miceClinical studiesNav currentsAdult malesExtracellular macrophage migration inhibitory factor (MIF) downregulates adipose hormone-sensitive lipase (HSL) and contributes to obesity
Chen L, Li L, Cui D, Huang Y, Tong H, Zabihi H, Wang S, Qi Y, Lakowski T, Leng L, Liu S, Wu H, Young L, Bucala R, Qi D. Extracellular macrophage migration inhibitory factor (MIF) downregulates adipose hormone-sensitive lipase (HSL) and contributes to obesity. Molecular Metabolism 2023, 79: 101834. PMID: 37935315, PMCID: PMC10700858, DOI: 10.1016/j.molmet.2023.101834.Peer-Reviewed Original ResearchMacrophage migration inhibitory factorExtracellular MIFHigh-fat dietHormone-sensitive lipaseDevelopment of obesityMigration inhibitory factorInhibitory factorRole of cytokinesExtracellular actionJNK phosphorylationMIF levelsSevere obesityHFD miceHFD feedingMIF antibodyWT miceAdipocyte hypertrophyCOP9 signalosome subunit 5Fat dietHSL expressionObesityAutocrine fashionHSL activationSensitive lipaseInhibitory actionBiomechanical Impact of Phosphate Wasting on Articular Cartilage Using the Murine Hyp Model of X‐linked hypophosphatemia
Macica C, Tommasini S. Biomechanical Impact of Phosphate Wasting on Articular Cartilage Using the Murine Hyp Model of X‐linked hypophosphatemia. JBMR Plus 2023, 7: e10796. PMID: 37808393, PMCID: PMC10556269, DOI: 10.1002/jbm4.10796.Peer-Reviewed Original ResearchWT miceHyp miceMechanical propertiesArticular cartilageTrabecular bone volumeProgression of osteoarthritisEtiology of osteoarthritisRange of motionSubchondral bone plateOral phosphateDegenerative osteoarthritisTibial articular cartilageDaily livingAC stiffnessBone volumeTherapeutic restorationOsteoarthritisStructural stiffnessCompressive loadingMicePeak loadTherapyCellular changesMineral deficitStress relaxationO-244 Mitochondrial fission factor (MFF) is required for female fertility and oocyte development
Hua R, Hai Z, Gu J, Guo C, Xiao Y, Zhao P, Su J, Yeung W, Wang T. O-244 Mitochondrial fission factor (MFF) is required for female fertility and oocyte development. Human Reproduction 2023, 38 DOI: 10.1093/humrep/dead093.298.Peer-Reviewed Original ResearchPremature ovarian failureGerminal vesicle stageFemale miceGV oocytesFollicle developmentMetaphase IIOocyte developmentMethods Female miceMetaphase II oocytesHematoxylin and eosin stainingFemale fertilityKnockout mouse modelOvarian failureWT miceAntral folliclesDrp1 protein expressionWT oocytesWT malesWider implicationsVesicle stageWT ovariesMouse modelEosin stainingGlobal knockoutFemale infertility
This site is protected by hCaptcha and its Privacy Policy and Terms of Service apply