2023
The active zone protein CLA-1 (Clarinet) bridges two subsynaptic domains to regulate presynaptic sorting of ATG-9
Xuan Z, Colón-Ramos D. The active zone protein CLA-1 (Clarinet) bridges two subsynaptic domains to regulate presynaptic sorting of ATG-9. Autophagy 2023, 19: 2807-2808. PMID: 37389488, PMCID: PMC10472863, DOI: 10.1080/15548627.2023.2229227.Peer-Reviewed Original ResearchConceptsATG-9Forward genetic screenAdaptor protein complexesIntegral synaptic vesicle proteinsActive zone proteinsSynaptic vesicle cycleCLA-1Synaptic vesicle proteinsGenetic screenPeriactive zoneAutophagosome biogenesisProtein complexesVesicle cycleVesicle proteinsLong isoformNovel roleSubsynaptic domainsZone proteinNeuronal synapsesSynaptic vesiclesProteinDistinct mechanismsVesiclesMutantsPresynaptic localizationThe active zone protein Clarinet regulates synaptic sorting of ATG-9 and presynaptic autophagy
Xuan Z, Yang S, Clark B, Hill S, Manning L, Colón-Ramos D. The active zone protein Clarinet regulates synaptic sorting of ATG-9 and presynaptic autophagy. PLOS Biology 2023, 21: e3002030. PMID: 37053235, PMCID: PMC10101500, DOI: 10.1371/journal.pbio.3002030.Peer-Reviewed Original ResearchConceptsATG-9Presynaptic autophagyAdaptor protein complexesZone proteinActive zone proteinsC. elegans neuronsSynaptic vesicle proteinsGenetic screenPeriactive zoneAutophagosome biogenesisCellular homeostasisProtein complexesVesicle proteinsGenetic analysisLong isoformNovel roleSynaptic vesiclesAutophagyDistinct mechanismsProteinVesiclesSortingCLA-1Abnormal accumulationActive zoneSynaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates
Park D, Wu Y, Wang X, Gowrishankar S, Baublis A, De Camilli P. Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates. Nature Communications 2023, 14: 455. PMID: 36709207, PMCID: PMC9884207, DOI: 10.1038/s41467-023-36081-3.Peer-Reviewed Original ResearchConceptsMembrane proteinsSV membrane proteinsVesicle membrane proteinEctopic expression systemSynaptic vesicle proteinsSynaptic vesicle clustersSV proteinsVesicle proteinsEctopic expressionExpression systemVesicle clustersSynapsinProteinLiquid-like propertiesVesiclesDistinct classesDual roleSynapsin 1FibroblastsATG9ASynapsesExpressionDistinct phasesNerve terminalsClusters
2022
A Quantitative Native Mass Spectrometry Platform for Deconstructing Hierarchical Organization of Membrane Proteins and Lipids
Panda A, Giska F, Brown C, Coleman J, Rothman J, Gupta K. A Quantitative Native Mass Spectrometry Platform for Deconstructing Hierarchical Organization of Membrane Proteins and Lipids. The FASEB Journal 2022, 36 DOI: 10.1096/fasebj.2022.36.s1.0r472.Peer-Reviewed Original ResearchMembrane proteinsOligomeric stateSpecific lipidsBiophysical propertiesSugar transporter proteinsPhysiological membranesBacterial plasma membraneTarget membrane proteinsLipid bilayer environmentSynaptic vesicle proteinsLipid compositionMS/MS capabilitiesProtein oligomerizationCellular signalingOligomeric organizationVesicle proteinsMembrane curvaturePlasma membraneMacromolecular assembliesTransporter proteinsNative massOligomeric populationMS/MS analysisRegulatory roleDiverse set
2020
PET Imaging of Synaptic Vesicle Protein 2A
Finnema S, Li S, Cai Z, Naganawa M, Chen M, Matuskey D, Nabulsi N, Esterlis I, Holmes S, Radhakrishnan R, Toyonaga T, Huang Y, Carson R. PET Imaging of Synaptic Vesicle Protein 2A. 2020, 993-1019. DOI: 10.1007/978-3-030-53176-8_29.ChaptersSynaptic vesicle protein 2APositron emission tomographyAntiepileptic drug levetiracetamInitial PET studiesPET imagingPET studiesSynaptic densityDrug levetiracetamHigh-affinity SV2A ligandsBrain regionsSynaptic density lossNeuropathological diseasesDisease-modifying therapiesLarge patient cohortMajor depressive disorderProtein 2APET imaging resultsPosttraumatic stress disorderPatient groupPatient cohortDepressive disorderClinical valueParkinson's diseaseEfficacy assessmentSynaptic vesicle proteins
2018
Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals
Jin E, Kiral F, Ozel M, Burchardt L, Osterland M, Epstein D, Wolfenberg H, Prohaska S, Hiesinger P. Live Observation of Two Parallel Membrane Degradation Pathways at Axon Terminals. Current Biology 2018, 28: 1027-1038.e4. PMID: 29551411, PMCID: PMC5944365, DOI: 10.1016/j.cub.2018.02.032.Peer-Reviewed Original ResearchConceptsSynaptic vesicle proteinsMembrane proteinsVesicle proteinsLive-imaging approachesPlasma membrane proteinsMembrane protein degradationIntact Drosophila brainsProtein degradation mechanismsMembrane cargoDegradative compartmentsNeuronal synaptobrevinProtein degradationDrosophila brainContinuous turnoverCell bodiesProteinDegradation pathwayLive observationDistinct classesSortingCargoCompartmentsSynaptobrevinDegradationRab7
2011
Rab3B protein is required for long-term depression of hippocampal inhibitory synapses and for normal reversal learning
Tsetsenis T, Younts T, Chiu C, Kaeser P, Castillo P, Südhof T. Rab3B protein is required for long-term depression of hippocampal inhibitory synapses and for normal reversal learning. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108: 14300-14305. PMID: 21844341, PMCID: PMC3161598, DOI: 10.1073/pnas.1112237108.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCA1 Region, HippocampalExcitatory Postsynaptic PotentialsExtinction, PsychologicalFearGene DeletionHippocampusInhibitory Postsynaptic PotentialsLong-Term Synaptic DepressionMiceMice, KnockoutModels, Neurologicalrab3 GTP-Binding ProteinsReversal LearningSynapsesSynaptic TransmissionConceptsLong-term potentiationI-LTDLong-term depressionInhibitory synapsesRab3-interacting moleculeEndocannabinoid-dependent long-term depressionLong-term plasticityRab3 isoformsKO miceMorris water-mazeFear conditioning assaysPresynaptically expressed long-term potentiationNMDA receptor-dependent long-term potentiationInitial phase of learningHippocampal inhibitory synapsesPhases of learningReversal learningWater-mazeGTP-dependent mannerSynaptic vesicle proteinsAcute slicesCA1 regionPresynaptic formShort-term plasticityExcitatory synapses
2008
RIM1α and RIM1β Are Synthesized from Distinct Promoters of the RIM1 Gene to Mediate Differential But Overlapping Synaptic Functions
Kaeser P, Kwon H, Chiu C, Deng L, Castillo P, Südhof T. RIM1α and RIM1β Are Synthesized from Distinct Promoters of the RIM1 Gene to Mediate Differential But Overlapping Synaptic Functions. Journal Of Neuroscience 2008, 28: 13435-13447. PMID: 19074017, PMCID: PMC2701653, DOI: 10.1523/jneurosci.3235-08.2008.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsExcitatory Postsynaptic PotentialsGTP-Binding ProteinsHippocampusMiceMice, KnockoutNeuronal PlasticityNeurotransmitter AgentsOrgan Culture TechniquesPatch-Clamp TechniquesPresynaptic TerminalsPromoter Regions, GeneticProtein IsoformsReverse Transcriptase Polymerase Chain ReactionSynapsesSynaptic TransmissionSynaptic VesiclesConceptsRIM1 geneActive zone proteinsKnock-out miceSynaptic vesicle primingPresynaptic long-term plasticitySynaptic vesicle proteinsNeurotransmitter releaseLong-term presynaptic plasticityVesicle primingActive zoneMultidomain proteinsSynaptotagmin-1Vesicle proteinsRIM1alphaN-terminusRIM1Plasma membranePresynaptic plasticityDeletionGenesSynaptic functionPresynaptic terminalsProteinIsoformsShort-term synaptic plasticity
2005
α-Synuclein Cooperates with CSPα in Preventing Neurodegeneration
Chandra S, Gallardo G, Fernández-Chacón R, Schlüter OM, Südhof TC. α-Synuclein Cooperates with CSPα in Preventing Neurodegeneration. Cell 2005, 123: 383-396. PMID: 16269331, DOI: 10.1016/j.cell.2005.09.028.Peer-Reviewed Original ResearchConceptsAbundant synaptic vesicle proteinsEndogenous synucleinNeuronal survivalNerve terminalsParkinson's diseaseProgressive neurodegenerationSynaptic vesicle proteinsAlpha-synucleinDownstream mechanismsNeurodegenerationVivo activitySNARE complex assemblyCSPalphaTransgenic expressionDiseaseMicePhysiological roleCochaperone functionVesicle proteinsSNARE proteinsComplex assemblyInjuryDeletionSynuclein
1998
Vesicle-associated brain myosin-V can be activated to catalyze actin-based transport
Evans L, Lee A, Bridgman P, Mooseker M. Vesicle-associated brain myosin-V can be activated to catalyze actin-based transport. Journal Of Cell Science 1998, 111: 2055-2066. PMID: 9645952, DOI: 10.1242/jcs.111.14.2055.Peer-Reviewed Original ResearchConceptsMyosin VVesicle proteinsTotal vesicle proteinSynaptic vesicle proteinsInitial fractionation stepSynaptic vesicle marker proteinActin transportBrain myosin-VOrganelle transportActin filament motilityOrganelle motorFunctional analysisVesicle fractionFunction-blocking antibodiesLocalization studiesMarker proteinsImmunoelectron microscopyMotility assaysMotor domainProteinVesiclesFilament motilityVesicle integrityActinVesicle surface
1994
Synaptic targeting of rabphilin-3A, a synaptic vesicle Ca2+/phospholipid-binding protein, depends on rab3A/3C
Li C, Takei K, Geppert M, Daniell L, Stenius K, Chapman E, Jahn R, De Camilli P, Südhof T. Synaptic targeting of rabphilin-3A, a synaptic vesicle Ca2+/phospholipid-binding protein, depends on rab3A/3C. Neuron 1994, 13: 885-898. PMID: 7946335, DOI: 10.1016/0896-6273(94)90254-2.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAmino Acid SequenceAnimalsBase SequenceBiological EvolutionBrain ChemistryConserved SequenceDNA, ComplementaryFluorescent Antibody TechniqueGlutathione TransferaseGTP-Binding ProteinsMiceMice, Mutant StrainsMicroscopy, ImmunoelectronMolecular Sequence DataNerve Tissue ProteinsNeuronsrab GTP-Binding Proteinsrab3 GTP-Binding ProteinsRatsRecombinant Fusion ProteinsVesicular Transport ProteinsConceptsGTP-dependent mannerSynaptic vesicle membraneRabphilin-3AVesicle membraneLow molecular weight GTPPeripheral membrane proteinsSynaptic vesiclesSynaptic vesicle dockingRab3A-deficient miceSynaptic vesicle proteinsMembrane recruitmentVesicle dockingPutative functionsMembrane proteinsWeight GTPVesicle proteinsN-terminusSynaptic targetingRab3CRab3AProteinVesiclesMembraneSynaptic patternsNormal levelsA role for synaptic vesicles in non‐neuronal cells: clues from pancreatic β cells and from chromaffin cells
Thomas‐Reetz A, De Camilli P. A role for synaptic vesicles in non‐neuronal cells: clues from pancreatic β cells and from chromaffin cells. The FASEB Journal 1994, 8: 209-216. PMID: 7907072, DOI: 10.1096/fasebj.8.2.7907072.Peer-Reviewed Original ResearchConceptsSynaptic-like microvesiclesSynaptic vesiclesAbundant synaptic vesicle proteinsVesicular carriersPancreatic beta cellsSynaptic vesicle proteinsVesicular trafficNon-neuronal cellsRecycling compartmentMembrane proteinsRecycling pathwayVesicle proteinsPancreatic β-cellsPeptide-secreting endocrine cellsMolecular mechanismsSpecialized subcompartmentBeta cellsPC12 cellsMembrane compositionVesiclesCell linesΒ-cellsOrganellesNeurotransmitter releaseNeurotransmitter-like substances
1993
Calcium/calmodulin-dependent protein kinase I. cDNA cloning and identification of autophosphorylation site.
Picciotto MR, Czernik AJ, Nairn AC. Calcium/calmodulin-dependent protein kinase I. cDNA cloning and identification of autophosphorylation site. Journal Of Biological Chemistry 1993, 268: 26512-26521. PMID: 8253780, DOI: 10.1016/s0021-9258(19)74343-9.Peer-Reviewed Original ResearchMeSH KeywordsAdrenal GlandsAmino Acid SequenceAnimalsBase SequenceBinding SitesBrainCalcium-Calmodulin-Dependent Protein Kinase Type 1Calcium-Calmodulin-Dependent Protein KinasesCattleCloning, MolecularDNA, ComplementaryEscherichia coliLiverLungMolecular Sequence DataPhosphorylationRatsRNA, MessengerSequence Homology, Amino AcidConceptsCaM kinase IKinase IProtein kinaseCatalytic domainThreonyl residuesFusion proteinGlutathione S-transferase fusion proteinS-transferase fusion proteinCAMP-dependent protein kinaseDependent protein kinase IComplete amino acid sequenceBovine brain cDNA libraryInvariant amino acidsAmino acidsSynapsin IAmino acid sequenceBrain cDNA libraryClass of enzymesSynaptic vesicle proteinsProtein kinase ICaM kinase IIAutophosphorylation sitesRNase protection assaysSingle geneCDNA libraryTraffic of synaptic vesicle proteins in polarized and nonpolarized cells
Cameron P, Mundigl O, De Camilli P. Traffic of synaptic vesicle proteins in polarized and nonpolarized cells. Journal Of Cell Science. Supplement 1993, 1993: 93-100. PMID: 8144709, DOI: 10.1242/jcs.1993.supplement_17.14.Peer-Reviewed Original ResearchConceptsSV proteinsSynaptic vesiclesSecretory organellesSpecialized secretory organellesSynaptic-like microvesiclesExo-endocytotic recyclingSynaptic vesicle proteinsNon-peptide hormonesEndosomal intermediatesEndocrine cellsNeuronal polarityNonpolarized cellsRecycling pathwayRegulated secretionVesicle proteinsTypical synaptic vesiclesFunctional similarityLarge dense-core vesiclesDense-core vesiclesNeuronal counterpartsVesicular carriersProtein synaptophysinVesicle storesMature neuronsProtein
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