2022
Dual-polarity voltage imaging of the concurrent dynamics of multiple neuron types
Kannan M, Vasan G, Haziza S, Huang C, Chrapkiewicz R, Luo J, Cardin J, Schnitzer M, Pieribone V. Dual-polarity voltage imaging of the concurrent dynamics of multiple neuron types. Science 2022, 378: eabm8797. PMID: 36378956, PMCID: PMC9703638, DOI: 10.1126/science.abm8797.Peer-Reviewed Original Research
2021
An active tethering mechanism controls the fate of vesicles
An SJ, Rivera-Molina F, Anneken A, Xi Z, McNellis B, Polejaev VI, Toomre D. An active tethering mechanism controls the fate of vesicles. Nature Communications 2021, 12: 5434. PMID: 34521845, PMCID: PMC8440521, DOI: 10.1038/s41467-021-25465-y.Peer-Reviewed Original ResearchConceptsArtificial tetherFull fusionOptogenetic controlExocyst complexExocyst functionVesicle tethersMembrane mergerTethering mechanismTarget membraneIntracellular fusionPlasma membraneMode of fusionVesicle fusionPhysiological relevanceLamellipodial expansionVesiclesTetheringExocystMembraneFusionFurther showTetherFusion modeFateComplexesTwo mechanisms drive pronuclear migration in mouse zygotes
Scheffler K, Uraji J, Jentoft I, Cavazza T, Mönnich E, Mogessie B, Schuh M. Two mechanisms drive pronuclear migration in mouse zygotes. Nature Communications 2021, 12: 841. PMID: 33547291, PMCID: PMC7864974, DOI: 10.1038/s41467-021-21020-x.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsCell NucleusFemaleFertilizationForminsGene Expression Regulation, DevelopmentalGenes, ReporterGreen Fluorescent ProteinsLuminescent ProteinsMaleMiceMice, Inbred C57BLMice, Inbred CBAMicrofilament ProteinsMicrotubulesMovementNerve Tissue ProteinsOocytesrab GTP-Binding ProteinsRed Fluorescent ProteinSpermatozoaZygoteConceptsFertilization coneDynein-dependent mannerNetwork of microtubulesFormin 2Fertilized mouse eggsParental genomesPaternal chromosomesParental chromosomesPronuclear migrationParental pronucleiMouse eggsMale pronucleusMouse zygotesFemale pronucleusChromosomePronucleusActinFertilized eggsPronucleiCell centreZygotes
2020
Resolving Cell Cycle Speed in One Snapshot with a Live-Cell Fluorescent Reporter
Eastman AE, Chen X, Hu X, Hartman AA, Morales A, Yang C, Lu J, Kueh HY, Guo S. Resolving Cell Cycle Speed in One Snapshot with a Live-Cell Fluorescent Reporter. Cell Reports 2020, 31: 107804. PMID: 32579930, PMCID: PMC7418154, DOI: 10.1016/j.celrep.2020.107804.Peer-Reviewed Original ResearchConceptsFluorescent reportersLive-cell fluorescent reporterCell cycle speedFluorescent timer proteinsCell proliferationCell cycle dynamicsRed fluorescent proteinFaster cycling cellsFate transitionsFusion reporterActive lociTimer proteinFluorescent proteinLength heterogeneityComplex tissuesHematopoietic cellsCycling cellsReporterFluorescence ratioCycle dynamicsProteinFunctional heterogeneityMouse strainsSolid tissuesCycle speed
2018
A stochastic epigenetic switch controls the dynamics of T-cell lineage commitment
Ng K, Yui M, Mehta A, Siu S, Irwin B, Pease S, Hirose S, Elowitz M, Rothenberg E, Kueh H. A stochastic epigenetic switch controls the dynamics of T-cell lineage commitment. ELife 2018, 7: e37851. PMID: 30457103, PMCID: PMC6245732, DOI: 10.7554/elife.37851.Peer-Reviewed Original ResearchConceptsDevelopmental fate transitionsIndividual gene lociCell fate decisionsUpstream transcription factorsT cell lineage commitmentIrreversible activationGenetic perturbationsFate transitionsIndividual lociSwitch-likeDistal enhancerEpigenetic switchFate decisionsGene locusFate commitmentTranscription factorsActive lociEpigenetic activationFluorescent proteinLociLineage commitmentActivation eventsCellsAllelesGenesCharacterizing a novel vGlut3-P2A-iCreER knockin mouse strain in cochlea
Li C, Shu Y, Wang G, Zhang H, Lu Y, Li X, Li G, Song L, Liu Z. Characterizing a novel vGlut3-P2A-iCreER knockin mouse strain in cochlea. Hearing Research 2018, 364: 12-24. PMID: 29706463, DOI: 10.1016/j.heares.2018.04.006.Peer-Reviewed Original ResearchMeSH KeywordsAcoustic StimulationAmino Acid Transport Systems, AcidicAnimalsCochleaEvoked Potentials, Auditory, Brain StemFemaleGene Knock-In TechniquesGenes, ReporterGenotypeHair Cells, Auditory, OuterIntegrasesLuminescent ProteinsMaleMice, Inbred C57BLMice, TransgenicNeurogliaPhenotypeReaction TimeReceptors, EstrogenSelective Estrogen Receptor ModulatorsSpiral GanglionTamoxifenTime FactorsConceptsInner hair cellsOuter hair cellsSpiral ganglion neuronsKnockin mouse strainGlia cellsVesicular glutamate transporter 3Mouse strainsHair cellsCochlear outer hair cellsRosa26-LSLVGLUT3 expressionGanglion neuronsVGLUT3Mouse cochleaTransporter 3Negative cellsMouse genetic studiesAntibody stainingTamoxifenUnique expression patternP2/P3Specific cell typesCell typesSitu hybridizationCochleaA gene-specific T2A-GAL4 library for Drosophila
Lee P, Zirin J, Kanca O, Lin W, Schulze K, Li-Kroeger D, Tao R, Devereaux C, Hu Y, Chung V, Fang Y, He Y, Pan H, Ge M, Zuo Z, Housden B, Mohr S, Yamamoto S, Levis R, Spradling A, Perrimon N, Bellen H. A gene-specific T2A-GAL4 library for Drosophila. ELife 2018, 7: e35574. PMID: 29565247, PMCID: PMC5898912, DOI: 10.7554/elife.35574.Peer-Reviewed Original ResearchConceptsFunction phenotypesIntrons of genesExpression of hundredsCell-type specificityLethal insertionsEssential genesGene functionEndogenous promoterLethal mutationsCDNA constructsGene expressionInserted cassettesGenesPowerful resourceExpressionPhenotypeSignal 3DrosophilaGAL4Severe lossIntronsChromosomesTranscriptionCRISPRPromoter
2017
How does solvation in the cell affect protein folding and binding?
Davis CM, Gruebele M, Sukenik S. How does solvation in the cell affect protein folding and binding? Current Opinion In Structural Biology 2017, 48: 23-29. PMID: 29035742, DOI: 10.1016/j.sbi.2017.09.003.Peer-Reviewed Original ResearchConceptsMild environmental changesProtein functionCellular processesProtein foldingCellular environmentProtein surface areaProtein studiesLive cellsEnvironmental changesNon-steric interactionsProtein processesNatural contextCellsInteraction typesSolute environmentFoldingProteinRecent examplesBindingRapid changesSolute compositionA cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells
Nagarkar-Jaiswal S, Manivannan S, Zuo Z, Bellen H. A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells. ELife 2017, 6: e26420. PMID: 28561736, PMCID: PMC5493436, DOI: 10.7554/elife.26420.Peer-Reviewed Original ResearchDetection of vulnerable neurons damaged by environmental insults in utero
Torii M, Sasaki M, Chang YW, Ishii S, Waxman SG, Kocsis JD, Rakic P, Hashimoto-Torii K. Detection of vulnerable neurons damaged by environmental insults in utero. Proceedings Of The National Academy Of Sciences Of The United States Of America 2017, 114: 2367-2372. PMID: 28123061, PMCID: PMC5338500, DOI: 10.1073/pnas.1620641114.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCerebral CortexElectroporationEmbryo, MammalianEthanolFemaleFlow CytometryGene Expression RegulationGenes, ReporterHeat Shock Transcription FactorsHeat-Shock ResponseLuminescent ProteinsMiceMice, TransgenicNeuronsNicotinePlasmidsPregnancyPrenatal Exposure Delayed EffectsResponse ElementsSuraminConceptsTransgenic reporter miceHeat shock factor 1 (HSF1) activationPrenatal exposureVulnerable neuronsPrognostic biomarkerReporter miceSusceptible individualsPostnatal disordersPostnatal lifeMultiple disordersNeuronsReporter assaysCellular damageEnvironmental insultsMiceHarmful agentsDisordersExposureTreatmentPhysical agentsFluorescence reporter systemPathogenesisSymptomsUteroAgentsAn ALS-Associated Mutant SOD1 Rapidly Suppresses KCNT1 (Slack) Na+-Activated K+ Channels in Aplysia Neurons
Zhang Y, Ni W, Horwich AL, Kaczmarek LK. An ALS-Associated Mutant SOD1 Rapidly Suppresses KCNT1 (Slack) Na+-Activated K+ Channels in Aplysia Neurons. Journal Of Neuroscience 2017, 37: 2258-2265. PMID: 28119399, PMCID: PMC5338764, DOI: 10.1523/jneurosci.3102-16.2017.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAplysiaBiophysicsCells, CulturedElectric StimulationEnzyme InhibitorsGanglia, InvertebrateHumansLuminescent ProteinsMembrane PotentialsMicroinjectionsMorpholinosMutationNerve Tissue ProteinsNeuronsPatch-Clamp TechniquesPotassium ChannelsPotassium Channels, Sodium-ActivatedRNA, Small InterferingSodiumSuperoxide Dismutase-1ConceptsAmyotrophic lateral sclerosisSuperoxide dismutase 1Mutant superoxide dismutase 1Potassium currentC-Jun N-terminal kinaseNeuronal excitabilityLateral sclerosisFatal adult-onset neurodegenerative diseaseN-terminal kinaseMutant human Cu/ZnNeuronal developmentDismutase 1Adult-onset neurodegenerative diseaseCurrent-clamp recordingsMotor neuron toxicityOutward potassium currentHuman Cu/ZnWild-type superoxide dismutase 1Neuron toxicityActivity of NaBag cell neuronsClamp recordingsNeuronal functionCell neuronsAction potentials
2016
Multiple cone pathways are involved in photic regulation of retinal dopamine
Qiao SN, Zhang Z, Ribelayga CP, Zhong YM, Zhang DQ. Multiple cone pathways are involved in photic regulation of retinal dopamine. Scientific Reports 2016, 6: 28916. PMID: 27356880, PMCID: PMC4928117, DOI: 10.1038/srep28916.Peer-Reviewed Original ResearchConceptsDopaminergic amacrine cellsRetinal dopamineAmacrine cellsBipolar cellsPhotosensitive retinal ganglion cellsCone-mediated responsesOFF bipolar cellsRetinal ganglion cellsParallel excitatoryInhibitory circuitsGanglion cellsExcitatory inputsDopamine releaseInhibitory responsesCone functionKey neurotransmitterNeural pathwaysCone pathwaysType 1Dye injectionDopamineRetinaPhotic regulationCellsDAC activityMicroRNA-dependent roles of Drosha and Pasha in the Drosophila larval ovary morphogenesis
Yang H, Li M, Hu X, Xin T, Zhang S, Zhao G, Xuan T, Li M. MicroRNA-dependent roles of Drosha and Pasha in the Drosophila larval ovary morphogenesis. Developmental Biology 2016, 416: 312-323. PMID: 27339292, DOI: 10.1016/j.ydbio.2016.06.026.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCytoskeletonDrosophila melanogasterDrosophila ProteinsEmbryonic Germ CellsFemaleGene Expression Regulation, DevelopmentalGene Knockdown TechniquesLarvaLuminescent ProteinsMicroRNAsMicroscopy, FluorescenceOrganogenesisOvaryRibonuclease IIIRNA InterferenceRNA-Binding ProteinsStem Cell NicheConceptsOvary morphogenesisPrimordial germ cellsLate third larval instarLoss of DroshaMiRNA pathway componentsCanonical miRNA pathwayGerm cell lineageMiRNA-mediated regulationGerm cell precursorsGenome-wide screeningTerminal filamentThird larval instarEarly larval stagesMiR-317Ovarian somaMiR-14MiR-8Argonaute 1Mutant phenotypeDicer-1MiRNA pathwayPGC differentiationGerm lineGSC nicheRegulatory networksTwo-colour live-cell nanoscale imaging of intracellular targets
Bottanelli F, Kromann EB, Allgeyer ES, Erdmann RS, Wood Baguley S, Sirinakis G, Schepartz A, Baddeley D, Toomre DK, Rothman JE, Bewersdorf J. Two-colour live-cell nanoscale imaging of intracellular targets. Nature Communications 2016, 7: 10778. PMID: 26940217, PMCID: PMC4785223, DOI: 10.1038/ncomms10778.Peer-Reviewed Original ResearchEstrogens Suppress a Behavioral Phenotype in Zebrafish Mutants of the Autism Risk Gene, CNTNAP2
Hoffman EJ, Turner KJ, Fernandez JM, Cifuentes D, Ghosh M, Ijaz S, Jain RA, Kubo F, Bill BR, Baier H, Granato M, Barresi MJ, Wilson SW, Rihel J, State MW, Giraldez AJ. Estrogens Suppress a Behavioral Phenotype in Zebrafish Mutants of the Autism Risk Gene, CNTNAP2. Neuron 2016, 89: 725-733. PMID: 26833134, PMCID: PMC4766582, DOI: 10.1016/j.neuron.2015.12.039.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedAutistic DisorderDisease Models, AnimalEstrogensGene Expression RegulationGenisteinGreen Fluorescent ProteinsHumansLarvaLuminescent ProteinsMembrane ProteinsMotor ActivityMutationNerve Tissue ProteinsPhenotypePhytoestrogensPsychotropic DrugsSeizuresSleep-Wake Transition DisordersVesicular Glutamate Transport Protein 2ZebrafishA painful neuropathy-associated Nav1.7 mutant leads to time-dependent degeneration of small-diameter axons associated with intracellular Ca2+ dysregulation and decrease in ATP levels
Rolyan H, Liu S, Hoeijmakers JG, Faber CG, Merkies IS, Lauria G, Black JA, Waxman SG. A painful neuropathy-associated Nav1.7 mutant leads to time-dependent degeneration of small-diameter axons associated with intracellular Ca2+ dysregulation and decrease in ATP levels. Molecular Pain 2016, 12: 1744806916674472. PMID: 27821467, PMCID: PMC5102167, DOI: 10.1177/1744806916674472.Peer-Reviewed Original ResearchConceptsSmall fiber neuropathySmall-diameter axonsTime-dependent degenerationDorsal root ganglion neuronsNerve fiber injuryNervous system disordersPrevious clinical reportsIntracellular calcium levelsMutant Nav1.7 channelsATP levelsAδ nerve fibersHigh altitude sicknessPainful neuropathyTime-dependent increaseFiber injuryClinical onsetGanglion neuronsOxygen species productionSystem disordersCalcium levelsClinical reportsDistal extremitiesIntracellular Ca2NeuropathyNav1.7 channels
2015
Comprehensive Corticospinal Labeling with mu-crystallin Transgene Reveals Axon Regeneration after Spinal Cord Trauma in ngr1−/− Mice
Fink KL, Strittmatter SM, Cafferty WB. Comprehensive Corticospinal Labeling with mu-crystallin Transgene Reveals Axon Regeneration after Spinal Cord Trauma in ngr1−/− Mice. Journal Of Neuroscience 2015, 35: 15403-15418. PMID: 26586827, PMCID: PMC4649010, DOI: 10.1523/jneurosci.3165-15.2015.Peer-Reviewed Original ResearchMeSH KeywordsAmidinesAnalysis of VarianceAnimalsAxonsBiotinCrystallinsDextransDisease Models, AnimalFunctional LateralityGene Expression RegulationGlial Fibrillary Acidic ProteinGPI-Linked ProteinsLuminescent ProteinsMiceMice, Inbred C57BLMice, Transgenicmu-CrystallinsMyelin ProteinsNerve RegenerationNogo Receptor 1Pyramidal TractsReceptors, Cell SurfaceRecovery of FunctionSpinal Cord InjuriesConceptsCorticospinal tractCST axonsTransgenic miceMotor tractsDextran amineFunctional deficitsSpinal cordAxon regenerationSpinal Cord Injury StudySpontaneous axon regenerationSpinal cord traumaNogo receptor 1Permanent functional deficitsPersistent functional deficitsBilateral pyramidotomyDorsal hemisectionMidthoracic cordCord traumaMotor pathwaysAdult CNSCST regenerationInjury studiesLesion siteRegenerating fibersNeural repairConvergence, Divergence, and Reconvergence in a Feedforward Network Improves Neural Speed and Accuracy
Jeanne JM, Wilson RI. Convergence, Divergence, and Reconvergence in a Feedforward Network Improves Neural Speed and Accuracy. Neuron 2015, 88: 1014-1026. PMID: 26586183, PMCID: PMC5488793, DOI: 10.1016/j.neuron.2015.10.018.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsAnimals, Genetically ModifiedAntigensAstrocytesBacterial ProteinsDrosophilaDrosophila ProteinsFibroblast Growth Factor 2Glutamic AcidLuminescent ProteinsModels, NeurologicalNerve NetNeuronsOlfactory PathwaysPatch-Clamp TechniquesProteoglycansReaction TimeRNA, Small InterferingSensory Receptor CellsSomatosensory CortexSynaptic TransmissionRegulatory T Cells in Tumor-Associated Tertiary Lymphoid Structures Suppress Anti-tumor T Cell Responses
Joshi NS, Akama-Garren EH, Lu Y, Lee DY, Chang GP, Li A, DuPage M, Tammela T, Kerper NR, Farago AF, Robbins R, Crowley DM, Bronson RT, Jacks T. Regulatory T Cells in Tumor-Associated Tertiary Lymphoid Structures Suppress Anti-tumor T Cell Responses. Immunity 2015, 43: 579-590. PMID: 26341400, PMCID: PMC4826619, DOI: 10.1016/j.immuni.2015.08.006.Peer-Reviewed Original ResearchMeSH KeywordsAdenocarcinomaAnimalsCell ProliferationCells, CulturedDendritic CellsFlow CytometryForkhead Transcription FactorsImmunohistochemistryLuminescent ProteinsLung NeoplasmsLymphocyte ActivationLymphocyte DepletionLymphocytes, Tumor-InfiltratingMice, TransgenicMicroscopy, ConfocalNeoplasmsT-LymphocytesT-Lymphocytes, RegulatoryConceptsTreg cell functionDendritic cellsTreg cellsAnti-tumor T cell responsesTumor-associated tertiary lymphoid structuresEndogenous immune responseTreg cell depletionRegulatory T cellsAnti-tumor responseT cell responsesCell functionTertiary lymphoid structuresHuman lung cancerPoor patient prognosisLymphoid structuresCell depletionCancer patientsLung cancerPatient prognosisLung adenocarcinomaT cellsImmune responseMouse modelTherapeutic benefitTumor destructionImprovement of HIV-1 and Human T Cell Lymphotropic Virus Type 1 Replication-Dependent Vectors via Optimization of Reporter Gene Reconstitution and Modification with Intronic Short Hairpin RNA
Shunaeva A, Potashnikova D, Pichugin A, Mishina A, Filatov A, Nikolaitchik O, Hu W, Mazurov D. Improvement of HIV-1 and Human T Cell Lymphotropic Virus Type 1 Replication-Dependent Vectors via Optimization of Reporter Gene Reconstitution and Modification with Intronic Short Hairpin RNA. Journal Of Virology 2015, 89: 10591-10601. PMID: 26269177, PMCID: PMC4580202, DOI: 10.1128/jvi.01940-15.Peer-Reviewed Original ResearchMeSH KeywordsBiological AssayCD4-Positive T-LymphocytesCell Linegamma-GlobinsGenes, ReporterGenetic EngineeringGenetic VectorsGreen Fluorescent ProteinsHEK293 CellsHIV-1Human T-lymphotropic virus 1HumansIntronsLuciferasesLuminescent ProteinsMicroRNAsRNA SplicingRNA, Small InterferingTransfectionVirionVirus ReplicationConceptsCell-to-cell transmissionCell-mediated infectionVirus type 1HIV-1Gamma-globinTarget cellsHTLV-1Infected cellsHIV-1-infected cellsHuman immunodeficiency virus type 1Human T-cell lymphotropic virus type 1Immunodeficiency virus type 1Type 1Human gamma-globin geneHIV-1 EnvCell-free infectionVirus-producing cellsGamma-globin genesInfected target cellsStudy HIV-1Reporter vectorReporter geneRetrovirus HTLV-1Level of reporter gene expressionShort hairpin RNA
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