Improvement of HIV-1 and Human T Cell Lymphotropic Virus Type 1 Replication-Dependent Vectors via Optimization of Reporter Gene Reconstitution and Modification with Intronic Short Hairpin RNA
Shunaeva A, Potashnikova D, Pichugin A, Mishina A, Filatov A, Nikolaitchik O, Hu W, Mazurov D. Improvement of HIV-1 and Human T Cell Lymphotropic Virus Type 1 Replication-Dependent Vectors via Optimization of Reporter Gene Reconstitution and Modification with Intronic Short Hairpin RNA. Journal Of Virology 2015, 89: 10591-10601. PMID: 26269177, PMCID: PMC4580202, DOI: 10.1128/jvi.01940-15.Peer-Reviewed Original ResearchMeSH KeywordsBiological AssayCD4-Positive T-LymphocytesCell Linegamma-GlobinsGenes, ReporterGenetic EngineeringGenetic VectorsGreen Fluorescent ProteinsHEK293 CellsHIV-1Human T-lymphotropic virus 1HumansIntronsLuciferasesLuminescent ProteinsMicroRNAsRNA SplicingRNA, Small InterferingTransfectionVirionVirus ReplicationConceptsCell-to-cell transmissionCell-mediated infectionVirus type 1HIV-1Gamma-globinTarget cellsHTLV-1Infected cellsHIV-1-infected cellsHuman immunodeficiency virus type 1Human T-cell lymphotropic virus type 1Immunodeficiency virus type 1Type 1Human gamma-globin geneHIV-1 EnvCell-free infectionVirus-producing cellsGamma-globin genesInfected target cellsStudy HIV-1Reporter vectorReporter geneRetrovirus HTLV-1Level of reporter gene expressionShort hairpin RNA
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