2025
Perturbational single-cell RNA sequencing of patient tumors in Merkel cell and small cell lung carcinomas.
Perry C, Frey A, Fei Y, Costa P, Wang J, Ghadermarzi S, Levine D, Koda A, Nassar A, Ding M, Nie Y, Cordero-Dumit T, Augert A, van Djik D, Olino K, Ishizuka J. Perturbational single-cell RNA sequencing of patient tumors in Merkel cell and small cell lung carcinomas. Journal Of Clinical Oncology 2025, 43: 2520-2520. DOI: 10.1200/jco.2025.43.16_suppl.2520.Peer-Reviewed Original ResearchSmall cell lung cancerMerkel cell carcinomaMonocyte-derived DCCD14+ monocytesT cellsPatient tumorsResponse to IFNImpact of immune checkpoint blockadeTumor-infiltrating T cellsCo-CultureSmall cell lung carcinomaTumor immunity cycleImmune checkpoint blockadeImmune cells co-culturedCell lung carcinomaInnate immune signaturesHuman tumor specimensCell lung cancerLymph node metastasisCell linesFactors associated with responseLong-term survivalAssociated with nonresponseStandard of careImmune samplesTriple negative breast cancer cells acquire lymphocyte proteins and genomic DNA during trogocytosis with T cells
Sivakoses A, Marcarian H, Arias A, Lam A, Ihedioha O, Santamaria-Barria J, Gurtner G, Bothwell A. Triple negative breast cancer cells acquire lymphocyte proteins and genomic DNA during trogocytosis with T cells. PeerJ 2025, 13: e19236. PMID: 40183054, PMCID: PMC11967428, DOI: 10.7717/peerj.19236.Peer-Reviewed Original ResearchConceptsTriple negative breast cancerTriple negative breast cancer cellsT cellsCancer cellsTumor cell expressionNegative breast cancer cellsNegative breast cancerGenomic DNABreast cancer cellsCluster of differentiationSpecific proteinsT cell-specific proteinsLymphoid markersBreast cancerDonor cellsCell expressionCD45Immune detectionFlow cytometryPatient samplesTrogocytosisCD45RACo-CultureImmunofluorescence imagingLymphocyte proteinAdvancements in techniques for human iPSC-derived cardiomyocytes maturation: mechanical and electrical stimulation approaches
Lu Y, Liu Y, Yan Y, Fooladi S, Qyang Y. Advancements in techniques for human iPSC-derived cardiomyocytes maturation: mechanical and electrical stimulation approaches. Biophysical Reviews 2025, 17: 169-183. PMID: 40060015, PMCID: PMC11885779, DOI: 10.1007/s12551-024-01267-6.Peer-Reviewed Original ResearchHuman induced pluripotent stem cell-derived cardiomyocytesMaturity of human-induced pluripotent stem cell-derived cardiomyocytesPluripotent stem cell-derived cardiomyocytesStem cell-derived cardiomyocytesIon channel expressionCell-derived cardiomyocytesChannel expressionCo-culture systemElectrophysiological propertiesAdult cardiomyocytesCardiomyocyte maturationSynchronous contractionProtein expressionGrowth factorSarcomere alignmentClinical practiceElectrical pacingElectrical stimulation techniquesMaturation protocolsCardiomyocytesCo-CultureRegenerative medicineElectrical stimulation approachesDisease modelsDrug screeningRetinal Pigmented Epithelium and the Outer Blood-Retinal Barrier
Rizzolo L. Retinal Pigmented Epithelium and the Outer Blood-Retinal Barrier. 2025, 587-601. DOI: 10.1016/b978-0-443-13820-1.00020-7.Peer-Reviewed Original ResearchRetinal pigment epitheliumOuter blood-retinal barrierBlood-retinal barrierNeurosensory retinaClaudin-19Pigment epitheliumTight junctionsRetinal pigment epithelium functionHuman retinal pigment epitheliumBarrier functionRetinal barrierCo-culture systemClaudin familyParacellular spaceFamily of transmembrane proteinsEpitheliumChoriocapillarisCo-CultureCapillary bedClaudinRetinaBidirectional interactionsGene expressionPhysiological functionsTransmembrane proteins
2024
Environmental fungi target thiol homeostasis to compete with Mycobacterium tuberculosis
Malhotra N, Oh S, Finin P, Medrano J, Andrews J, Goodwin M, Markowitz T, Lack J, Boshoff H, Barry C. Environmental fungi target thiol homeostasis to compete with Mycobacterium tuberculosis. PLOS Biology 2024, 22: e3002852. PMID: 39625876, PMCID: PMC11614215, DOI: 10.1371/journal.pbio.3002852.Peer-Reviewed Original ResearchConceptsBiosynthetic gene clusterGene clusterWhole-genome sequencingSphagnum peat bogsCollection of FungiMycobacterium tuberculosisGenome sequenceThiol stressMycobacterial speciesMtb cellsSphagnum bogFungiA productionCo-CultureInduced expressionThiol homeostasisRNAseqMtbPeat bogsSphagnumBogNatural productsMycothiolGenesVulnerable targetsHuman IPSC-Derived Microglia Sense and Dampen Hyperexcitability of Cortical Neurons Carrying the Epilepsy-Associated SCN2A-L1342P Mutation
Que Z, Olivero-Acosta M, Robinson M, Chen I, Zhang J, Wettschurack K, Wu J, Xiao T, Otterbacher C, Shankar V, Harlow H, Hong S, Zirkle B, Wang M, Cui N, Mandal P, Chen X, Deming B, Halurkar M, Zhao Y, Rochet J, Xu R, Brewster A, Wu L, Yuan C, Skarnes W, Yang Y. Human IPSC-Derived Microglia Sense and Dampen Hyperexcitability of Cortical Neurons Carrying the Epilepsy-Associated SCN2A-L1342P Mutation. Journal Of Neuroscience 2024, 45: e2027232024. PMID: 39557580, PMCID: PMC11735681, DOI: 10.1523/jneurosci.2027-23.2024.Peer-Reviewed Original ResearchNeuronal excitabilityHyperexcitable neuronsHuman microgliaCo-CultureVoltage-gated sodium channel NaV1.2Neuronal activityRepetitive action potential firingRodent models of seizuresBrain-resident immune cellsSodium channel expressionInfluence neuronal excitabilityAction potential firingHyperexcitability of cortical neuronsModulates neuronal excitabilityEpilepsy-causing mutationsSodium channel Nav1.2Resident immune cellsAbnormal neuronal activityPresence of microgliaSuppression of seizuresModulate neuronal activityDensity of sodium channelsModels of seizuresPresence of neuronsAxon initial segmentEnhancing human capillary tube network assembly and maturation through upregulated expression of pericyte-derived TIMP-3
Yrigoin K, Bernard K, Castaño M, Cleaver O, Sumanas S, Davis G. Enhancing human capillary tube network assembly and maturation through upregulated expression of pericyte-derived TIMP-3. Frontiers In Cell And Developmental Biology 2024, 12: 1465806. PMID: 39544367, PMCID: PMC11560913, DOI: 10.3389/fcell.2024.1465806.Peer-Reviewed Original ResearchTIMP-3Endothelial cellsHuman pericytesPresence of doxycyclineCollagen type IVCo-culture modelIncreasing tube lengthHuman endothelial cellsStabilization networkTissue inhibitorNetwork assemblyPericyte recruitmentCapillary assemblyCo-assemblyType IVUpregulated expressionPericytesCo-CultureTube lengthDoxycycline additionEC-pericyte interactionsTube widthDoxycyclineNetwork of capillary tubesEnterococcus faecalis-derived adenine enhances enterohaemorrhagic Escherichia coli Type 3 Secretion System-dependent virulence
Martins F, Rosay T, Rajan A, Carter H, Turocy T, Mejia A, Crawford J, Maresso A, Sperandio V. Enterococcus faecalis-derived adenine enhances enterohaemorrhagic Escherichia coli Type 3 Secretion System-dependent virulence. Nature Microbiology 2024, 9: 2448-2461. PMID: 38965331, PMCID: PMC11585081, DOI: 10.1038/s41564-024-01747-1.Peer-Reviewed Original ResearchMeSH KeywordsAdenineAnimalsCoculture TechniquesEnterococcus faecalisEnterocytesEnterohemorrhagic Escherichia coliEscherichia coli InfectionsEscherichia coli ProteinsGastrointestinal MicrobiomeGene Expression Regulation, BacterialHemolysin ProteinsHost-Pathogen InteractionsHumansHypoxanthineMiceType III Secretion SystemsVirulenceVirulence FactorsXanthineConceptsT3SS gene expressionType 3 secretion systemGene expressionAE lesion formationPromote colonization resistanceE. faecalisAdenine biosynthesisEHEC virulenceEffector translocationBacterial geneticsColonization resistanceEnteric pathogensT3SSLesion formationEHECSupplementation experimentsVirulencePathway activationAdenineEnhanced pathogenesisHost responseCo-InfectionExpressionCo-CultureTranscriptomeAbstract P37: Establishment of Gastric Organoids Biobank and Its Usage
Hagihara T, Huang K, Sundar R, Uchihara T, Ng C, Tay S, Tan A, So J, Tan P. Abstract P37: Establishment of Gastric Organoids Biobank and Its Usage. Cancer Research 2024, 84: p37-p37. DOI: 10.1158/1538-7445.fcs2023-p37.Peer-Reviewed Original Research
2018
In vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cells
Harrison S, Sozen B, Zernicka-Goetz M. In vitro generation of mouse polarized embryo-like structures from embryonic and trophoblast stem cells. Nature Protocols 2018, 13: 1586-1602. PMID: 29988106, DOI: 10.1038/s41596-018-0005-x.Peer-Reviewed Original ResearchConceptsTrophoblast stem cellsEmbryonic stem cellsMouse embryonic stem cellsExtra-embryonic tissuesStem cellsGerm-layer specificationEmbryos in vitroExtracellular matrixStem cell typesIn vitro generationPost-implantation developmentEmbryo-like structuresEarly embryos in vitroBody planMammalian developmentMammalian embryogenesisTissue in vitroEarly embryosEmbryonic tissues in vitroAggregates of embryonic stem cellsCell numberCo-CultureEmbryonic architectureCell typesCulture conditions
2016
Valve Interstitial Cells Act in a Pericyte Manner Promoting Angiogensis and Invasion by Valve Endothelial Cells
Arevalos C, Berg J, Nguyen J, Godfrey E, Iriondo C, Grande-Allen K. Valve Interstitial Cells Act in a Pericyte Manner Promoting Angiogensis and Invasion by Valve Endothelial Cells. Annals Of Biomedical Engineering 2016, 44: 2707-2723. PMID: 26905695, PMCID: PMC4983529, DOI: 10.1007/s10439-016-1567-9.Peer-Reviewed Original ResearchConceptsValvular interstitial cellsValve endothelial cellsROCK-dependent mannerInvasion of MatrigelVasculogenic networksCell organizationCo-CulturePhenotypic changesInvasive spheroidsCalcific aortic valve diseaseROCK activityControl cellsCell typesProgression of calcific aortic valve diseaseValve cellsCell culturesCell-activeEndothelial cellsPhenotypeCellsLong-term cultureSpheroid formationPericyte-like phenotypeBasal levelsInvasion
2006
Defective CD107a Surface Expression Heralds Munc13-4 Defect and Discriminates between Genetic Subtypes of Familial Hemophagocytic Lymphohistiocytosis (FHL).
Aricò M, Marcenaro S, Gallo F, Martini S, Santoro A, Griffiths G, Moretta L, Pende D. Defective CD107a Surface Expression Heralds Munc13-4 Defect and Discriminates between Genetic Subtypes of Familial Hemophagocytic Lymphohistiocytosis (FHL). Blood 2006, 108: 1248. DOI: 10.1182/blood.v108.11.1248.1248.Peer-Reviewed Original ResearchFamilial hemophagocytic lymphohistiocytosisNK cellsCD107a expressionFHL3 patientsNatural killerIL-2Genetic subtypesB-EBV cell linesCell surface CD107a expressionSurface expression of CD107aCases of HLHInvestigated natural killerSurface CD107a expressionExpression of CD107aNK cell populationCD56+ cellsNK cell defectPolyclonal NK cellsCentral nervous system alterationsLyse target cellsAnalysis of PBMCsCo-CultureInfiltration of lymphocytesNervous system alterationsMunc13-4 protein
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