Marc C Llaguno, PhD
Research ScientistDownloadHi-Res Photo
Cards
Appointments
Neuroscience
Primary
Additional Titles
Site Director, Cryo-Electron Microscopy YSM, Neuroscience
Contact Info
Appointments
Neuroscience
Primary
Additional Titles
Site Director, Cryo-Electron Microscopy YSM, Neuroscience
Contact Info
Appointments
Neuroscience
Primary
Additional Titles
Site Director, Cryo-Electron Microscopy YSM, Neuroscience
Contact Info
About
Titles
Research Scientist
Site Director, Cryo-Electron Microscopy YSM, Neuroscience
Appointments
Neuroscience
Research ScientistPrimary
Other Departments & Organizations
- Electron Microscopy
- Neuroscience
Education & Training
- PhD
- University of Pennsylvania, Physics (2004)
- BS
- University of the Philippines, Physics (1997)
Research
Overview
Medical Research Interests
Cryoelectron Microscopy
ORCID
0000-0001-9568-9565- View Lab Website
CCMI Electron Microscopy Lab
Research at a Glance
Yale Co-Authors
Frequent collaborators of Marc C Llaguno's published research.
Publications Timeline
A big-picture view of Marc C Llaguno's research output by year.
Research Interests
Research topics Marc C Llaguno is interested in exploring.
Elizabeth Jonas, MD
Nelli Mnatsakanyan, PhD
Chenxiang Lin, PhD
35Publications
3,980Citations
Cryoelectron Microscopy
Publications
2022
Mitochondrial ATP synthase c-subunit leak channel triggers cell death upon loss of its F1 subcomplex
Mnatsakanyan N, Park HA, Wu J, He X, Llaguno MC, Latta M, Miranda P, Murtishi B, Graham M, Weber J, Levy RJ, Pavlov EV, Jonas EA. Mitochondrial ATP synthase c-subunit leak channel triggers cell death upon loss of its F1 subcomplex. Cell Death & Differentiation 2022, 29: 1874-1887. PMID: 35322203, PMCID: PMC9433415, DOI: 10.1038/s41418-022-00972-7.Peer-Reviewed Original ResearchMeSH Keywords and ConceptsConceptsMitochondrial permeability transitionATP synthase c-subunitCell deathMitochondrial ATP synthaseChannel activityCellular energy productionLeak channelsVoltage-gated ion channelsF1 subcomplexATP synthaseC subunitInner membraneProkaryotic hostsCell stressPermeability transitionIon channelsGating mechanismOsmotic changesLarge conductanceC-ringChannels triggersNeuronal deathF1SubcomplexOsmotic gradient
2020
Structural and Pharmacological Characterization of the Mitochondrial Permeability Transition Pore: A Megachannel Formed by F1FO ATP Synthase
Mnatsakanyan N, Llaguno M, Yang Y, Yan Y, Weber J, Sigworth F, Jonas E. Structural and Pharmacological Characterization of the Mitochondrial Permeability Transition Pore: A Megachannel Formed by F1FO ATP Synthase. Biophysical Journal 2020, 118: 1a. DOI: 10.1016/j.bpj.2019.11.198.Peer-Reviewed Original Research
2019
A mitochondrial megachannel resides in monomeric F1FO ATP synthase
Mnatsakanyan N, Llaguno MC, Yang Y, Yan Y, Weber J, Sigworth FJ, Jonas EA. A mitochondrial megachannel resides in monomeric F1FO ATP synthase. Nature Communications 2019, 10: 5823. PMID: 31862883, PMCID: PMC6925261, DOI: 10.1038/s41467-019-13766-2.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsATP synthase monomersMitochondrial permeability transition poreATP synthaseGiant unilamellar vesiclesMitochondrial megachannelOligomeric stateSmall unilamellar vesiclesF1Fo-ATP synthaseMitochondrial ATP synthaseMitochondrial inner membraneCryo-EM density mapsPermeability transition porePorcine heart mitochondriaUnilamellar vesiclesInner membraneMPTP activityTransition poreElectron cryomicroscopyChannel activityLipid compositionDimer formationHeart mitochondriaSynthaseChannel formationVesiclesP4‐512: EXCITOTOXIC NEURONAL DEATH INDUCING MEGACHANNEL RESIDES IN MONOMERIC F1FO ATP SYNTHASE
Mnatsakanyan N, Llaguno M, Yang Y, Yan Y, Sigworth F, Jonas E. P4‐512: EXCITOTOXIC NEURONAL DEATH INDUCING MEGACHANNEL RESIDES IN MONOMERIC F1FO ATP SYNTHASE. Alzheimer's & Dementia 2019, 15: p1509-p1510. DOI: 10.1016/j.jalz.2019.08.058.Peer-Reviewed Original ResearchMitochondrial Megachannel Resides in Monomeric ATP Synthase
Mnatsakanyan N, Park H, Jing W, Llaguno M, Murtishi B, Latta M, Davis E, Miranda P, Yang Y, Sigworth F, Jonas E. Mitochondrial Megachannel Resides in Monomeric ATP Synthase. Biophysical Journal 2019, 116: 156a. DOI: 10.1016/j.bpj.2018.11.863.Peer-Reviewed Original ResearchCitations
2017
Placing and shaping liposomes with reconfigurable DNA nanocages
Zhang Z, Yang Y, Pincet F, Llaguno M, Lin C. Placing and shaping liposomes with reconfigurable DNA nanocages. Nature Chemistry 2017, 9: 653-659. PMID: 28644472, PMCID: PMC5542812, DOI: 10.1038/nchem.2802.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsMembrane-bound vesiclesDNA cagesRegulated deformationsDNA nanocagesMembrane curvatureMembrane fusionConformational changesBiological membranesCell membraneLipid bilayer membranesMembrane mechanicsVesiclesDiverse structuresMembraneCellsBilayer membranesVersatile toolDelivery vesiclesToroid shapeLiposome shape
2016
bSUM: A bead-supported unilamellar membrane system facilitating unidirectional insertion of membrane proteins into giant vesicles
Zheng H, Lee S, Llaguno M, Jiang Q. bSUM: A bead-supported unilamellar membrane system facilitating unidirectional insertion of membrane proteins into giant vesicles. Journal Of Cell Biology 2016, 212: 2122oia1. DOI: 10.1083/jcb.2122oia1.Peer-Reviewed Original Research
2015
bSUM: A bead-supported unilamellar membrane system facilitating unidirectional insertion of membrane proteins into giant vesicles
Zheng H, Lee S, Llaguno MC, Jiang QX. bSUM: A bead-supported unilamellar membrane system facilitating unidirectional insertion of membrane proteins into giant vesicles. The Journal Of General Physiology 2015, 147: 77-93. PMID: 26712851, PMCID: PMC4692488, DOI: 10.1085/jgp.201511448.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsMembrane proteinsIon channelsLipid compositionLipid bilayersMembrane systemLipid-protein interactionsVitro membrane systemSingle-molecule imagingVoltage-gated ion channelsGiant vesiclesUnilamellar naturePlanar electrodesPlanar lipid bilayersSuch proteinsMicroscopic imagingBiophysical analysisFast gating kineticsUnidirectional insertionProteinChannel activityProtein orientationGating kineticsModel systemBilayersSpecific ligandsStructural Studies of IP3R by Cryoem
Jiang Q, Zheng H, Llaguno M. Structural Studies of IP3R by Cryoem. Biophysical Journal 2015, 108: 340a-341a. DOI: 10.1016/j.bpj.2014.11.1866.Peer-Reviewed Original Research
2014
Chemically functionalized carbon films for single molecule imaging
Llaguno MC, Xu H, Shi L, Huang N, Zhang H, Liu Q, Jiang QX. Chemically functionalized carbon films for single molecule imaging. Journal Of Structural Biology 2014, 185: 405-417. PMID: 24457027, PMCID: PMC3990355, DOI: 10.1016/j.jsb.2014.01.006.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsCarbon surfaceNanometer-thick carbon filmsBiological complexesCarbon filmsIndividual macromolecular complexesFunctionalized filmsBioactive ligandsChemical linkageSingle-molecule imagingSubnanogram levelActive siteStrong oxidationNanomolar levelsDNA/RNAMolecule imagingNegative-stain EMElectron microscopic imagingSelective enrichmentComplexesLigandsMacromolecular complexesFilmsProtein GSubnanomolar concentrationsLong-term storage