Madison Strine, PhD
Postdoctoral AssociateAbout
Research
Publications
Featured Publications
Intestinal tuft cell immune privilege enables norovirus persistence
Strine M, Fagerberg E, Darcy P, Barrón G, Filler R, Alfajaro M, D'Angelo-Gavrish N, Wang F, Graziano V, Menasché B, Damo M, Wang Y, Howitt M, Lee S, Joshi N, Mucida D, Wilen C. Intestinal tuft cell immune privilege enables norovirus persistence. Science Immunology 2024, 9: eadi7038-eadi7038. PMID: 38517952, PMCID: PMC11555782, DOI: 10.1126/sciimmunol.adi7038.Peer-Reviewed Original ResearchConceptsCD8+ T cellsIntestinal tuft cellsT cellsTufted CellsViral persistenceSite of viral persistenceChemosensory epithelial cellsNormal antigen presentationImmune-privileged nicheIntestinal stem cellsMemory phenotypeImmune privilegeImmune escapeReporter miceAntigen presentationChronic infectionCytotoxic capacityEpithelial cellsNorovirus infectionStem cellsCell interactionsInfectionCell survivalEnteric microbesCellsDYRK1A promotes viral entry of highly pathogenic human coronaviruses in a kinase-independent manner
Strine M, Cai W, Wei J, Alfajaro M, Filler R, Biering S, Sarnik S, Chow R, Patil A, Cervantes K, Collings C, DeWeirdt P, Hanna R, Schofield K, Hulme C, Konermann S, Doench J, Hsu P, Kadoch C, Yan Q, Wilen C. DYRK1A promotes viral entry of highly pathogenic human coronaviruses in a kinase-independent manner. PLOS Biology 2023, 21: e3002097. PMID: 37310920, PMCID: PMC10263356, DOI: 10.1371/journal.pbio.3002097.Peer-Reviewed Original ResearchConceptsGenome-wide CRISPR/Cas9 screenCRISPR/Cas9 screenPathogenic human coronavirusesKinase-independent mannerRegulated kinase 1AProviral host factorNovel drug targetsMultiple cell typesDNA accessibilityHost factorsKinase functionHuman coronavirusesHost genesDistal enhancerNovel regulatorCas9 screenKinase 1AGene expressionNeuronal developmentDYRK1ADrug targetsDiverse coronavirusesProviral activityCell typesSevere acute respiratory syndrome coronavirus 2Tuft-cell-intrinsic and -extrinsic mediators of norovirus tropism regulate viral immunity
Strine M, Alfajaro M, Graziano V, Song J, Hsieh L, Hill R, Guo J, VanDussen K, Orchard R, Baldridge M, Lee S, Wilen C. Tuft-cell-intrinsic and -extrinsic mediators of norovirus tropism regulate viral immunity. Cell Reports 2022, 41: 111593. PMID: 36351394, PMCID: PMC9662704, DOI: 10.1016/j.celrep.2022.111593.Peer-Reviewed Original ResearchTuft cells are key mediators of interkingdom interactions at mucosal barrier surfaces
Strine MS, Wilen CB. Tuft cells are key mediators of interkingdom interactions at mucosal barrier surfaces. PLOS Pathogens 2022, 18: e1010318. PMID: 35271673, PMCID: PMC8912186, DOI: 10.1371/journal.ppat.1010318.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsInterkingdom interactionsTuft cellsCell biologyImmune responseMicrobial activationMicrobial sensingCell abundanceMucosal barrier surfacesAntiviral adaptive immune responsesType 2 immune responsesCell heterogeneityExquisite specificityMucosal barrier integrityAdaptive immune responsesMurine norovirusHuman healthKey orchestratorsMicrobial infectionsPathogenic bacteriaBroad intraFlavivirus replicationKey mediatorContext of coinfectionTissue repairImmune evasion
2025
RNA N-glycosylation enables immune evasion and homeostatic efferocytosis
Graziano V, Porat J, Ah Kioon M, Mejdrová I, Matz A, Lebedenko C, Chai P, Pluvinage J, Ricci-Azevedo R, Harrison A, Wright S, Wang X, Strine M, Wang P, Wilson M, Vanaja S, Zhou B, Barrat F, Carell T, Flynn R, Rathinam V. RNA N-glycosylation enables immune evasion and homeostatic efferocytosis. Nature 2025, 645: 784-792. PMID: 40770106, PMCID: PMC12845878, DOI: 10.1038/s41586-025-09310-6.Peer-Reviewed Original ResearchConceptsNon-inflammatory clearanceN-glycansSmall RNAsInnate immune sensingApoptotic cellsEndogenous small RNAsFunctional significanceProduction of type I interferonsDe-N-glycosylationInnate immune activationEndosomal networkInnate immune responseClearance of dead cellsN-glycosylationRNA sensorsType I interferonToll-like receptorsRNAGlycoRNACell surfaceGenetic deletionI interferonDead cellsAcp3UAutoinflammatory responsesBugged before birth?: How maternal microbes reprogram offspring immunity
Strine M, Konnikova L. Bugged before birth?: How maternal microbes reprogram offspring immunity. Cell Host & Microbe 2025, 33: 1036-1038. PMID: 40639328, DOI: 10.1016/j.chom.2025.06.003.Peer-Reviewed Original ResearchExploring mucosal immune development and function in utero
Strine M, Vahkal B, St Denis K, Konnikova L. Exploring mucosal immune development and function in utero. Trends In Immunology 2025, 46: 502-504. PMID: 40518358, PMCID: PMC12255524, DOI: 10.1016/j.it.2025.05.004.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsNorovirus co-opts NINJ1 for selective protein secretion
Song J, Zhang L, Moon S, Fang A, Wang G, Gheshm N, Loeb S, Cao P, Wallace J, Alfajaro M, Strine M, Beatty W, Jamieson A, Orchard R, Robinson B, Nice T, Wilen C, Orvedahl A, Reese T, Lee S. Norovirus co-opts NINJ1 for selective protein secretion. Science Advances 2025, 11: eadu7985. PMID: 40020060, PMCID: PMC11870086, DOI: 10.1126/sciadv.adu7985.Peer-Reviewed Original ResearchConceptsPlasma membrane ruptureDamage-associated molecular patternsNS1 secretionNinjurin-1Programmed Cell DeathAmino acid residuesViral replication sitesViral protein NS1CRISPR screensIntracellular viral proteinsMutagenesis studiesMembrane ruptureProtein NS1Unconventional pathwayCaspase-3Protein secretionViral proteinsReplication sitesCell deathMolecular patternsGenetic ablationNS1Pharmaceutical inhibitionDAMP releaseProtein
2024
Human Milk Supports Robust Intestinal Organoid Growth, Differentiation, and Homeostatic Cytokine Production
Smith L, Santiago E, Eke C, Gu W, Wang W, Llivichuzhca-Loja D, Kehoe T, St Denis K, Strine M, Taylor S, Tseng G, Konnikova L. Human Milk Supports Robust Intestinal Organoid Growth, Differentiation, and Homeostatic Cytokine Production. Gastro Hep Advances 2024, 3: 1030-1042. PMID: 39529649, PMCID: PMC11550179, DOI: 10.1016/j.gastha.2024.07.007.Peer-Reviewed Original ResearchHuman milk supplementationTNF-related apoptosis inducing ligandDonor human milkLevels of leukemia inhibitory factorNecrotizing enterocolitisCytokine productionInflammatory immune signaturesComplications of prematurityHuman milkChromogranin A stainingSevere gastrointestinal complicationsMilk supplementationCell cycle-promoting genesIntestinal organoidsApoptosis inducing ligandIntestinal epithelial proliferationIntestinal epithelial growthGrowth factor analysisCleaved caspase 3Preterm infantsGestational ageLeukemia inhibitory factorImmune signaturesImmune landscapeHydrolyzed formula
2023
Bacillus subtilis Phages Related to SIOphi from Desert Soils of the Southwest United States
Magness L, Delesalle V, Vill A, Strine M, Chaudhry B, Lichty K, Guffey A, DeCurzio J, Krukonis G. Bacillus subtilis Phages Related to SIOphi from Desert Soils of the Southwest United States. PHAGE 2023, 4: 165-172. PMID: 40134792, PMCID: PMC11932521, DOI: 10.1089/phage.2023.0021.Peer-Reviewed Original ResearchPutative protein coding genesDouble-stranded DNA genomeBacillus subtilis phagePhage host rangeLow GC contentProtein coding genesDiversity of phagesAmino acid similarityPhage clusterPhage evolutionPhage genomeGC contentUnique genesBacillus phagesCoding genesDNA genomeReplication genesModel organismsPhageHost rangeMicrobial dynamicsGenetic differencesGenomeGenesDesert soils