About
Research
Publications
2024
Bridge-like lipid transfer protein family member 2 suppresses ciliogenesis
Parolek J, Burd C. Bridge-like lipid transfer protein family member 2 suppresses ciliogenesis. Molecular Biology Of The Cell 2024, 35: br11. PMID: 38536441, PMCID: PMC11151097, DOI: 10.1091/mbc.e24-02-0065.Peer-Reviewed Original ResearchConceptsLipid transfer proteinsFamily member 2RPE-1 cellsSuppressed ciliogenesisTubular endosomal networkMembrane contact sitesNegative regulator of ciliogenesisRegulator of ciliogenesisDrosophila melanogaster</i>Evolutionary conserved proteinMember 2Primary cilium biogenesisRPE-1Endosomal networkGenetic interactionsTubular endosomesCilium biogenesisProtein familyStructure predictionContact sitesEndoplasmic reticulumDomain-containingPreweaning lethalityNegative regulatorCiliogenesis
2023
Cholesterol-dependent homeostatic regulation of very long chain sphingolipid synthesis
Kim Y, Mavodza G, Senkal C, Burd C. Cholesterol-dependent homeostatic regulation of very long chain sphingolipid synthesis. Journal Of Cell Biology 2023, 222: e202308055. PMID: 37787764, PMCID: PMC10547602, DOI: 10.1083/jcb.202308055.Peer-Reviewed Original ResearchConceptsPlasma membraneEpidermal growth factor receptor signalingMembrane lipid homeostasisGrowth factor receptor signalingAcute cholesterol depletionCellular cholesterol homeostasisPlasma membrane cholesterol contentCellular cholesterol levelsMembrane lipid packingSterol-responsive elementMembrane cholesterol contentProtein translationCytoplasmic leafletCholesterol depletionSphingolipid synthesisGolgi apparatusProtein pathwayResponsive elementLipid homeostasisRegulatory axisReceptor signalingLysosome membraneSphingomyelin synthesisAccumulation of cholesterolMetabolic axisLipid Sorting and Organelle Identity.
Kim Y, Burd C. Lipid Sorting and Organelle Identity. Cold Spring Harbor Perspectives In Biology 2023, 15: a041397. PMID: 37487627, PMCID: PMC10547387, DOI: 10.1101/cshperspect.a041397.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsTrafficking of lipidsSynthesis of sphingolipidsLipid transfer proteinOrganelle identityMembrane traffickingOrganelle homeostasisSoluble lipid transfer proteinsBulk membrane propertiesOrganelle functionGolgi networkLipid sortingNet membrane chargeOrganelle membranesPlasma membraneEndoplasmic reticulumParticular lipidsOrganellesTransfer proteinLeaflet asymmetryEssential roleBulk membraneCholesterol fluxTraffickingMembraneSecretory
2022
Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathway
Sokoya T, Parolek J, Foged M, Danylchuk D, Bozan M, Sarkar B, Hilderink A, Philippi M, Botto L, Terhal P, Mäkitie O, Piehler J, Kim Y, Burd C, Klymchenko A, Maeda K, Holthuis J. Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathway. ELife 2022, 11: e79278. PMID: 36102623, PMCID: PMC9531943, DOI: 10.7554/elife.79278.Peer-Reviewed Original ResearchConceptsSecretory pathwayER export signalPatient-derived fibroblastsFull enzymatic activityPhysical membrane propertiesExport signalSterol gradientLipid landscapeMammalian cellsCholesterol organizationLipid orderEnzymatic activityLipid distributionOsteogenic cellsPathwayBone phenotypeSMS2Glycerophospholipid profilePathogenic variantsCellsVariantsSphingomyelinSkeletal dysplasiaERSphingolipids
2019
Syndecan-1 Mediates Sorting of Soluble Lipoprotein Lipase with Sphingomyelin-Rich Membrane in the Golgi Apparatus
Sundberg EL, Deng Y, Burd CG. Syndecan-1 Mediates Sorting of Soluble Lipoprotein Lipase with Sphingomyelin-Rich Membrane in the Golgi Apparatus. Developmental Cell 2019, 51: 387-398.e4. PMID: 31543446, PMCID: PMC6832887, DOI: 10.1016/j.devcel.2019.08.014.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkSphingomyelin-rich membranesTransmembrane domainSecretory pathwayVesicular transport carriersIntegral membrane proteinsProtein transmembrane domainBiosynthetic secretory pathwaySyndecan-1Heparan sulfate chainsLipoprotein lipaseSorting receptorSecretion pathwayMembrane proteinsGolgi membranesProteoglycan syndecan-1Protein cargoSecretory vesiclesPlasma membraneGolgi apparatusSpecific sequencesTransport carriersSulfate chainsLipid compositionEnzyme lipoprotein lipase
2018
Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network
Deng Y, Pakdel M, Blank B, Sundberg EL, Burd CG, von Blume J. Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network. Developmental Cell 2018, 47: 464-478.e8. PMID: 30393074, PMCID: PMC6261503, DOI: 10.1016/j.devcel.2018.10.012.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkTGN membranesCell surface transportProtein sortingTrafficking pathwaysSecretion pathwaySecretory proteinsSphingomyelin synthesisSorting mechanismLipid synthesisProteinVesicular carriersCab45Sphingomyelin contentNew lipidSortingPrincipal functionMembranePathwayCore componentLipidsGolgiSurface transportExportActivityCell-Penetrating Peptide Mediates Intracellular Membrane Passage of Human Papillomavirus L2 Protein to Trigger Retrograde Trafficking
Zhang P, da Silva G, Deatherage C, Burd C, DiMaio D. Cell-Penetrating Peptide Mediates Intracellular Membrane Passage of Human Papillomavirus L2 Protein to Trigger Retrograde Trafficking. Cell 2018, 174: 1465-1476.e13. PMID: 30122350, PMCID: PMC6128760, DOI: 10.1016/j.cell.2018.07.031.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceCapsid ProteinsCell-Penetrating PeptidesEndosomesGolgi ApparatusGreen Fluorescent ProteinsHEK293 CellsHeLa CellsHuman papillomavirus 16HumansMutagenesisOncogene Proteins, ViralProtein TransportRecombinant Fusion ProteinsSequence AlignmentVirus AttachmentVirus InternalizationConceptsCell-penetrating peptidesTrans-Golgi networkNormal cell physiologyL2 proteinRetrograde transport pathwayShort protein segmentsHPV L2 proteinTrafficking factorsRetrograde traffickingCationic cell-penetrating peptidesCell physiologyEndosomal membranesProtein segmentsC-terminusBiological roleNon-enveloped virusesRetrograde pathwayL2 capsid proteinsMembrane passageCell penetrating peptideCapsid proteinViral proteinsProteinRetromerTransport pathwaysLipid trafficking by yeast Snx4 family SNX-BAR proteins promotes autophagy and vacuole membrane fusion
Ma M, Kumar S, Purushothaman L, Babst M, Ungermann C, Chi RJ, Burd CG. Lipid trafficking by yeast Snx4 family SNX-BAR proteins promotes autophagy and vacuole membrane fusion. Molecular Biology Of The Cell 2018, 29: 2190-2200. PMID: 29949447, PMCID: PMC6249802, DOI: 10.1091/mbc.e17-12-0743.Peer-Reviewed Original ResearchConceptsVacuole fusionAutophagy pathwaySNX-BAR proteinsCore autophagy machinerySelective autophagy pathwaysHomotypic vacuole fusionVacuole membrane fusionPhosphatidylserine-containing membranesRetrograde transport carriersTurnover of macromoleculesAutophagy intermediatesGlycerophospholipid homeostasisNonselective autophagyBAR domainEndosomal sortingAutophagy machineryFusion competenceLipid traffickingPhosphatidylserine-rich membranesCoat proteinMembrane fusionVacuole membranePhosphatidylethanolamine biosynthesisYeast cellsPhosphatidylethanolamine synthesis
2016
Sphingomyelin is sorted at the trans Golgi network into a distinct class of secretory vesicle
Deng Y, Rivera-Molina FE, Toomre DK, Burd CG. Sphingomyelin is sorted at the trans Golgi network into a distinct class of secretory vesicle. Proceedings Of The National Academy Of Sciences Of The United States Of America 2016, 113: 6677-6682. PMID: 27247384, PMCID: PMC4914164, DOI: 10.1073/pnas.1602875113.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkSynthesis of sphingomyelinGolgi networkSecretory vesiclesPlasma membraneQuantitative live-cell imagingVesicular transport carriersSorting of proteinsGlycophosphatidylinositol-anchored proteinsPore-forming toxinsLive-cell imagingInterorganelle traffickingAbundant sphingolipidIntracellular traffickingSecretory proteinsSM transportTransport carriersProteinCell imagingTraffickingDistinct classesSpecific carrierVesiclesPrincipal functionSorting
2011
Roles of lipids in Golgi sorting
Burd C, Wood C, Hung C. Roles of lipids in Golgi sorting. The FASEB Journal 2011, 25: 83.2-83.2. DOI: 10.1096/fasebj.25.1_supplement.83.2.Peer-Reviewed Original ResearchGolgi compartmentPI4KGenetic interaction studiesGolgi membrane proteinLocation of enzymesMedial Golgi compartmentActivation of mTORRole of lipidsBiosynthetic cargoMembrane proteinsSorting reactionActing enzymesGolgi stacksKey regulatorOncogenic transformationGolgi apparatusGolgiVps74Trans compartmentEnzymeCompartmentsInteraction studiesProper identityCarbohydrate moietyOrthologs
Academic Achievements & Community Involvement
News & Links
News
- October 15, 2023Source: Cold Spring Harbor Perspectives in Biology
New review article by Research Scientist Yeongho Kim and Professor Chris Burd
- October 03, 2023Source: Journal of Cell Biology
Kim and Burd discover a role for sphingolipids in cellular cholesterol homeostasis.
- March 21, 2023
Meet Our Speakers: Fikadu Tafesse
- July 13, 2022Source: Molecular Biology of the Cell
Protein and lipid trafficking in epithelial cells