2007
Directed differentiation of human embryonic stem cells towards a pancreatic cell fate
Shim J, Kim S, Woo D, Kim S, Oh C, McKay R, Kim J. Directed differentiation of human embryonic stem cells towards a pancreatic cell fate. Diabetologia 2007, 50: 1228-1238. PMID: 17457565, DOI: 10.1007/s00125-007-0634-z.Peer-Reviewed Original ResearchMeSH KeywordsActivinsAnimalsCell Culture TechniquesCell DifferentiationDiabetes Mellitus, ExperimentalEmbryonic Stem CellsGene Expression RegulationHomeodomain ProteinsHumansMaleMiceMice, Inbred BALB CMice, NudePancreasReverse Transcriptase Polymerase Chain ReactionStem Cell TransplantationTrans-ActivatorsTransplantation, HeterologousTretinoinConceptsHuman embryonic stem cellsPdx1-positive cellsForkhead box protein A2Embryonic stem cellsEndodermal cellsCaudal-type homeobox transcription factor 2Differentiation of hESCsCell typesPancreatic cell fateRetinoic acidStem cellsMature cell typesEmbryonic germ layersEmbryoid body formationPancreatic cell typesBox protein A2Transcription factor 2Vivo pancreatic developmentEpigenetic signalsCell fateEctodermal differentiationDuodenal homeobox 1Pancreatic fateGene 17Gene expression
2006
Involvement of the Fas–Fas ligand system and active caspase-3 in abnormal apoptosis in human testes with maturation arrest and Sertoli cell–only syndrome
Kim S, Yoon Y, Park Y, Seo J, Kim J. Involvement of the Fas–Fas ligand system and active caspase-3 in abnormal apoptosis in human testes with maturation arrest and Sertoli cell–only syndrome. Fertility And Sterility 2006, 87: 547-553. PMID: 17123522, DOI: 10.1016/j.fertnstert.2006.07.1524.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisAzoospermiaCaspase 3Fas Ligand ProteinFas ReceptorGene ExpressionHumansIn Situ Nick-End LabelingInfertility, MaleMaleProspective StudiesReverse Transcriptase Polymerase Chain ReactionSertoli CellsSyndromeTestisUp-RegulationConceptsMaturation arrestActive caspase-3Sertoli cellsCaspase-3Testicular expressionDUTP nick end labeling stainingCases of SCOTerminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) stainingNick end labeling stainingTransferase-mediated dUTP nick-end labeling stainingTesticular biopsy specimensTestes of patientsGerm cellsTranscription-polymerase chain reactionFas-Fas ligand systemRoutine clinical purposesHyperplastic interstitial cellsExpression of FasLExpression of FasBiopsy specimensMAIN OUTCOMEObstructive azoospermiaImmunohistochemical analysisIntense immunostainingLabeling stainingNonylphenol and Octylphenol-Induced Apoptosis in Human Embryonic Stem Cells Is Related to Fas-Fas Ligand Pathway
Kim S, Kim B, Shim J, Gil J, Yoon Y, Kim J. Nonylphenol and Octylphenol-Induced Apoptosis in Human Embryonic Stem Cells Is Related to Fas-Fas Ligand Pathway. Toxicological Sciences 2006, 94: 310-321. PMID: 16984955, DOI: 10.1093/toxsci/kfl114.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisCell LineCell SurvivalCoculture TechniquesDose-Response Relationship, DrugEmbryonic Stem CellsEnvironmental PollutantsFas Ligand ProteinFas ReceptorGene ExpressionHumansMicePhenolsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerConceptsHuman embryonic stem cellsUndifferentiated hES cellsEmbryonic stem cellsHES cellsNeural progenitor cellsEarly embryonic developmentStem cellsProgenitor cellsToxic stress responseChemical-induced apoptosisFas ligand protein expressionFas-Fas ligand pathwayCell replacement therapyEmbryonic developmentCaspase activationES cellsCaspase-8Stress responseDevelopmental stagesDNA fragmentationCell typesFas-FasL interactionCommon environmental contaminantsApoptosisProtein expression