2014
Engineering Human Peripheral Blood Stem Cell Grafts that Are Depleted of Naïve T Cells and Retain Functional Pathogen-Specific Memory T Cells
Bleakley M, Heimfeld S, Jones LA, Turtle C, Krause D, Riddell SR, Shlomchik W. Engineering Human Peripheral Blood Stem Cell Grafts that Are Depleted of Naïve T Cells and Retain Functional Pathogen-Specific Memory T Cells. Transplantation And Cellular Therapy 2014, 20: 705-716. PMID: 24525279, PMCID: PMC3985542, DOI: 10.1016/j.bbmt.2014.01.032.Peer-Reviewed Original ResearchConceptsPeripheral blood stem cellsHematopoietic cell transplantationMemory T cellsStem cell graftsT cellsCell graftsPathogen-specific memory T cellsPeripheral blood stem cell graftsAllogeneic stem cell graftsBlood stem cell graftsNaïve T cell subsetsAllogeneic hematopoietic cell transplantationFrequent major complicationCentral memory phenotypeT cell subsetsBlood stem cellsNaïve T cellsOpportunistic pathogenCommon opportunistic pathogenStem cellsHost diseaseHCT outcomesEffector cytokinesMajor complicationsMemory phenotype
2012
Nonhematopoietic Cells are the Primary Source of Bone Marrow‐Derived Lung Epithelial Cells
Kassmer SH, Bruscia EM, Zhang P, Krause DS. Nonhematopoietic Cells are the Primary Source of Bone Marrow‐Derived Lung Epithelial Cells. Stem Cells 2012, 30: 491-499. PMID: 22162244, PMCID: PMC3725285, DOI: 10.1002/stem.1003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacterial ProteinsBone Marrow CellsBone Marrow TransplantationCell SeparationEpithelial CellsGene ExpressionLuminescent ProteinsLungMiceMice, 129 StrainMice, Inbred C57BLMice, KnockoutMicroscopy, ConfocalPulmonary Surfactant-Associated Protein CRecombinant ProteinsSingle-Cell AnalysisConceptsLung epithelial cellsNonhematopoietic cellsBM cellsEpithelial cellsBone marrowLungs of miceType 2 pneumocytesNonhematopoietic stem cellsNonhematopoietic fractionAdult BMPrimitive stem cell populationNull miceProgenitor cellsMiceStem cell populationCell populationsMarrowStem cellsMultiple tissuesHematopoietic stemBMCellsPrevious studiesEngraftmentLung
2009
C/EBPε directs granulocytic-vs-monocytic lineage determination and confers chemotactic function via Hlx
Halene S, Gaines P, Sun H, Zibello T, Lin S, Khanna-Gupta A, Williams SC, Perkins A, Krause D, Berliner N. C/EBPε directs granulocytic-vs-monocytic lineage determination and confers chemotactic function via Hlx. Experimental Hematology 2009, 38: 90-103.e4. PMID: 19925846, PMCID: PMC2827304, DOI: 10.1016/j.exphem.2009.11.004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone Marrow CellsCCAAT-Enhancer-Binding ProteinsCell DifferentiationCell LineChemotaxis, LeukocyteGene ExpressionGranulocyte-Macrophage Colony-Stimulating FactorGranulocytesHematopoietic Stem CellsHomeodomain ProteinsMiceMice, KnockoutMonocytesMyelopoiesisNeutrophilsReceptors, ChemokineTranscription FactorsTransduction, GeneticConceptsKO cellsNew regulatory functionCommon myeloid progenitorsNeutrophil-specific granule deficiencyProgenitor cell lineCell linesRestoration of expressionDifferentiated cell linesSpecific granule deficiencyLineage-specific cell surface antigensLineage decisionsLineage determinationEpsilon geneCCAAT enhancerDeficiency phenotypeRegulatory functionsChemotaxis defectIntermediate cell typeKO bone marrowPerformed expressionNeutrophil differentiationCell typesFunctional studiesNeutrophil maturationMyeloid progenitors
2008
Bone Marrow–derived Cells and Stem Cells in Lung Repair
Krause DS. Bone Marrow–derived Cells and Stem Cells in Lung Repair. Annals Of The American Thoracic Society 2008, 5: 323-327. PMID: 18403327, PMCID: PMC2645242, DOI: 10.1513/pats.200712-169dr.Peer-Reviewed Original ResearchConceptsMarrow-derived epithelial cellsEpithelial cellsBM cellsTissue injuryBone marrow-derived cellsBM-derived cellsMarrow-derived cellsPotential clinical utilityBronchiolar epithelial cellsType II pneumocytesLung damageTracheal epithelial cellsLung repairClinical utilityGI tractBone marrowTissue damagePeer-reviewed studiesNonhematopoietic cell typesBeneficial effectsPotential mechanismsTissue repairLungInjuryTissue microenvironment
2007
Limitations of Green Fluorescent Protein as a Cell Lineage Marker
Swenson ES, Price JG, Brazelton T, Krause DS. Limitations of Green Fluorescent Protein as a Cell Lineage Marker. Stem Cells 2007, 25: 2593-2600. PMID: 17615263, DOI: 10.1634/stemcells.2007-0241.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood CellsCattleCell LineageChickensCytomegalovirusFemaleFluorescent Antibody TechniqueGene ExpressionGenes, ReporterGenes, SyntheticGreen Fluorescent ProteinsHumansImmunoenzyme TechniquesMaleMiceMice, Inbred C57BLOrgan SpecificityRegulatory Sequences, Nucleic AcidTissue DistributionTransgenesVisceraConceptsSmall intestineMouse strainsPeripheral blood cellsTransgenic mouse strainReporter mouse strainPrimary rabbit antibodiesDonor originHuman ubiquitin C promoterImmunohistochemical stainingSolid organsCell lineage markersCell lineagesUBC-GFP miceUbiquitin C promoterChicken beta-actinFlow cytometryBlood cellsEnhanced green fluorescent protein (EGFP) reporterMiceOne-tissueAdult liverTissue sectionsIntestineLineage markersRabbit antibodies
2006
SALL4, a novel oncogene, is constitutively expressed in human acute myeloid leukemia (AML) and induces AML in transgenic mice
Ma Y, Cui W, Yang J, Qu J, Di C, Amin HM, Lai R, Ritz J, Krause DS, Chai L. SALL4, a novel oncogene, is constitutively expressed in human acute myeloid leukemia (AML) and induces AML in transgenic mice. Blood 2006, 108: 2726-2735. PMID: 16763212, PMCID: PMC1895586, DOI: 10.1182/blood-2006-02-001594.Peer-Reviewed Original ResearchMeSH KeywordsAlternative SplicingAnimalsApoptosisBase SequenceBeta CateninCloning, MolecularColony-Forming Units AssayDNA, ComplementaryDNA, NeoplasmDNA-Binding ProteinsGene ExpressionHematopoiesisHumansLeukemia, Myeloid, AcuteMiceMice, TransgenicMyelodysplastic SyndromesNeoplasm TransplantationOncogenesProtein IsoformsRNA, MessengerRNA, NeoplasmSignal TransductionTranscription FactorsWnt ProteinsConceptsAcute myeloid leukemiaMyeloid leukemiaMurine modelTransgenic miceHuman primary acute myeloid leukemiaMDS/acute myeloid leukemiaPrimary acute myeloid leukemiaHuman acute myeloid leukemiaLeukemia stem cellsAML transformationMyelodysplastic syndromePolymerase chain reactionWnt/beta-catenin pathwayZinc finger transcriptional factorNovel oncogeneBeta-catenin pathwayLeukemogenic potentialConstitutive expressionChain reactionPathway's roleLeukemiaSALL4MiceStem cellsMouse marrow
2004
Lineage specificity of gene expression patterns
Kluger Y, Tuck DP, Chang JT, Nakayama Y, Poddar R, Kohya N, Lian Z, Nasr A, Halaban HR, Krause DS, Zhang X, Newburger PE, Weissman SM. Lineage specificity of gene expression patterns. Proceedings Of The National Academy Of Sciences Of The United States Of America 2004, 101: 6508-6513. PMID: 15096607, PMCID: PMC404075, DOI: 10.1073/pnas.0401136101.Peer-Reviewed Original ResearchConceptsGene expression patternsExpression patternsExpression dataGene Ontology databaseTree of relationshipsStem cellsBroad functional categoriesHematopoietic cell populationsMRNA expression dataHematopoietic programBioCarta databasesGenome databaseLineage choiceHematopoietic stem cellsKyoto EncyclopediaDifferent lineagesFunctional categoriesLineage developmentCell lineagesGene expressionLineage specificityOligonucleotide microarraysFunctional pathwaysLineage discriminationOntology database
2002
Marrow-Derived Cells as Vehicles for Delivery of Gene Therapy to Pulmonary Epithelium
Grove JE, Lutzko C, Priller J, Henegariu O, Theise ND, Kohn DB, Krause DS. Marrow-Derived Cells as Vehicles for Delivery of Gene Therapy to Pulmonary Epithelium. American Journal Of Respiratory Cell And Molecular Biology 2002, 27: 645-651. PMID: 12444022, DOI: 10.1165/rcmb.2002-0056rc.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone Marrow TransplantationFemaleGene ExpressionGenetic TherapyGreen Fluorescent ProteinsHematopoietic Stem Cell TransplantationIndicators and ReagentsLuminescent ProteinsLung DiseasesMiceMice, Inbred C57BLProtein PrecursorsProteolipidsRespiratory MucosaRetroviridaeRNA, MessengerConceptsBone marrow-derived stem cellsLung epithelial cellsLung epitheliumEpithelial cellsMarrow-derived stem cellsGene therapyBMSC transplantationLung diseaseFemale miceAirway epitheliumAlveolar spaceLong-term transgene expressionMale marrowPulmonary epitheliumLong-term gene expressionGene therapy applicationsGene expressionPulmonary airwaysAdenoviral vectorEpitheliumMarrowTherapyTransient gene expressionNonviral vectorsStem cells
1998
Normal neutrophil differentiation and secondary granule gene expression in the EML and MPRO cell lines.
Lawson ND, Krause DS, Berliner N. Normal neutrophil differentiation and secondary granule gene expression in the EML and MPRO cell lines. Experimental Hematology 1998, 26: 1178-85. PMID: 9808058.Peer-Reviewed Original ResearchConceptsNeutrophil differentiationDominant-negative retinoic acid receptor alphaCell linesNormal neutrophil differentiationRetinoic acid receptor alphaCD11b/CD18Macrophage colony-stimulating factorDownregulation of CD34Granulocyte/macrophage colony-stimulating factorTrans retinoic acidAcid receptor alphaColony-stimulating factorNeutrophil gelatinaseGranulocytic differentiation programReceptor alphaSerum-free mediumAppropriate cytokinesMyeloid lineageRetinoic acidInduces expressionLeukemic myelopoiesisHL60MyelopoiesisNeutrophilic differentiationDifferentiation program
1997
Multilineage gene expression precedes commitment in the hemopoietic system.
Hu M, Krause D, Greaves M, Sharkis S, Dexter M, Heyworth C, Enver T. Multilineage gene expression precedes commitment in the hemopoietic system. Genes & Development 1997, 11: 774-785. PMID: 9087431, DOI: 10.1101/gad.11.6.774.Peer-Reviewed Original ResearchConceptsGene expression programsMultilineage gene expressionLineage specificationExpression programsGene activityLocus activationMultipotential stateGene expressionCytokine receptorsHemopoietic stemGranulocytic lineageProgenitor cellsSingle-cell RT-PCRSame cellsHemopoietic systemRT-PCRExclusive commitmentCell RT-PCRCellsLineagesCoexpressionDifferentiationExpressionStemActivation