2006
Expression of the basolateral Na–K–Cl cotransporter during mouse nephrogenesis and embryonic development
Heuvel G, Payne J, Igarashi P, Forbush B. Expression of the basolateral Na–K–Cl cotransporter during mouse nephrogenesis and embryonic development. Gene Expression Patterns 2006, 6: 1000-1006. PMID: 16814616, DOI: 10.1016/j.modgep.2006.04.004.Peer-Reviewed Original ResearchConceptsIn situ hybridizationNa-K-Cl cotransporterDorsal root ganglionBranching ureteric budEndocapillary cellsPeripheral nervous systemRoot ganglionTooth budsNasal epitheliumMouse nephrogenesisChoroid plexusSubmandibular glandMature nephronsUreteric budNorthern blot analysisSLC12A2Nervous systemMesenchymal cellsNKCC1Blot analysisMetanephroiNephric structuresGlomeruliMiceEmbryonic development
1996
Na:K:2Cl Cotransporter (NKCC) of Intestinal Epithelial Cells SURFACE EXPRESSION IN RESPONSE TO cAMP*
D'Andrea L, Lytle C, Matthews J, Hofman P, Forbush B, Madara J. Na:K:2Cl Cotransporter (NKCC) of Intestinal Epithelial Cells SURFACE EXPRESSION IN RESPONSE TO cAMP*. Journal Of Biological Chemistry 1996, 271: 28969-28976. PMID: 8910547, DOI: 10.1074/jbc.271.46.28969.Peer-Reviewed Original ResearchConceptsChloride secretionSurface expressionBumetanide-sensitive NaMonoclonal antibodiesIntestinal chloride secretionIncreased surface expressionCell surface expressionEpithelial cell lineNKCC activityT84 monolayersCotransport activityAgonist stimulationCAMP agonistsEpithelial uptakeCotransporterBasolateral domainIntestinal epitheliaSurface biotinylationNKCCConfocal localizationApproximately 6-foldCell linesF-actin cytoskeletonSecretionImmunoelectron microscopy