Packaging and Uncoating of CRISPR/Cas Ribonucleoproteins for Efficient Gene Editing with Viral and Non-Viral Extracellular Nanoparticles
Mazurov D, Ramadan L, Kruglova N. Packaging and Uncoating of CRISPR/Cas Ribonucleoproteins for Efficient Gene Editing with Viral and Non-Viral Extracellular Nanoparticles. Viruses 2023, 15: 690. PMID: 36992399, PMCID: PMC10056905, DOI: 10.3390/v15030690.Peer-Reviewed Original ResearchConceptsTarget cellsExtracellular nanoparticlesFunctional genomic studiesGene editingCell-type specificityGene editing applicationsGenomic studiesViral particle productionEfficient gene editingDisease correctionPackaging mechanismRibonucleoproteinNon-viralEditing efficiencyOff-target activityEffector nucleasesClinical utilityCurrent delivery systemsOff-target effectsLipofection methodCRISPR/CasPrimary cellsCRISPR/Cas ribonucleoproteinDNA exposureGenes