2024
Lymphocyte Phosphatase-Associated Phosphoprotein (LPAP) as a CD45 Protein Stability Regulator
Kruglova N, Mazurov D, Filatov A. Lymphocyte Phosphatase-Associated Phosphoprotein (LPAP) as a CD45 Protein Stability Regulator. Biochemistry (Moscow) 2024, 89: 912-922. PMID: 38880651, DOI: 10.1134/s0006297924050110.Peer-Reviewed Original ResearchConceptsLymphocyte phosphatase-associated phosphoproteinExpression level of CD45Protein stability regulationSurface expression of CD45Expression of CD45Levels of CD45Binding partnersPhosphatase CD45Lymphoid cellsLymphocyte activationCD45 proteinSurface expressionCD45Biochemical evidenceK562 cellsStability regulationLymphocytesPhosphoprotein
2019
Distinct Requirements for HIV-1 Accessory Proteins during Cell Coculture and Cell-Free Infection
Zotova A, Atemasova A, Pichugin A, Filatov A, Mazurov D. Distinct Requirements for HIV-1 Accessory Proteins during Cell Coculture and Cell-Free Infection. Viruses 2019, 11: 390. PMID: 31027334, PMCID: PMC6563509, DOI: 10.3390/v11050390.Peer-Reviewed Original ResearchMeSH KeywordsCD4-Positive T-LymphocytesCell LineCell-Free SystemCells, CulturedCoculture TechniquesGene Expression Regulation, ViralGene Knockdown TechniquesHIV InfectionsHIV-1Human Immunodeficiency Virus ProteinsHumansJurkat CellsMutationNef Gene Products, Human Immunodeficiency VirusViral Regulatory and Accessory ProteinsVirus ReplicationConceptsCell-free infectionHIV-1 infectionCell coculturesHIV-1Cell-to-cell transmission of HIV-1Transmission of HIV-1Nonlymphoid cellsHIV-1 accessory proteinHIV-1 replicationMeasuring virus replicationVirus replicationCell-to-cell transmissionEffector cellsLymphoid cellsMode of transmissionTarget cellsProducing cellsStages of infectionEnhanced replicationAccessory proteinsInfectionCocultureInfected cellsReporter vectorViral particles
2017
Gene Editing in Human Lymphoid Cells: Role for Donor DNA, Type of Genomic Nuclease and Cell Selection Method
Zotova A, Lopatukhina E, Filatov A, Khaitov M, Mazurov D. Gene Editing in Human Lymphoid Cells: Role for Donor DNA, Type of Genomic Nuclease and Cell Selection Method. Viruses 2017, 9: 325. PMID: 29099045, PMCID: PMC5707532, DOI: 10.3390/v9110325.Peer-Reviewed Original ResearchConceptsAdeno-associated virus integration site 1Virus type 1Lymphoid cellsHuman lymphoid cellsNon-homologous end joiningZinc finger nucleasesHIV-1Human immunodeficiency virus type 1Immunodeficiency virus type 1Type 1Replication of HIV-1Virus integration site 1Human T-cell leukemia virus type 1B cell linesDifficult-to-transfect cellsGene function validationHTLV-1Human TOn-target efficiencyCEM cellsOff-target effectsTarget lociGene-edited cellsHomologous recombinationOn-target activity
2011
Role of O-Glycosylation and Expression of CD43 and CD45 on the Surfaces of Effector T Cells in Human T Cell Leukemia Virus Type 1 Cell-to-Cell Infection
Mazurov D, Ilinskaya A, Heidecker G, Filatov A. Role of O-Glycosylation and Expression of CD43 and CD45 on the Surfaces of Effector T Cells in Human T Cell Leukemia Virus Type 1 Cell-to-Cell Infection. Journal Of Virology 2011, 86: 2447-2458. PMID: 22171268, PMCID: PMC3302266, DOI: 10.1128/jvi.06993-11.Peer-Reviewed Original ResearchConceptsJurkat cellsVirus type 1Expression of CD43Short hairpin RNAT cellsHTLV-1O-glycosylationHIV-1O-glycan elongationType 1Human immune deficiency virus type 1Effector T cellsHIV-1 infectionHuman T-cell leukemia virus type 1Cell-to-cell infectionSurface of T cellsCell-cell contactJurkat T cellsHTLV-1 infectionHTLV-1 proteinsHTLV-1 particlesCell surface antigensChaperone CosmcInfectious structuresCosmc geneAnalysis of Tn antigenicity with a panel of new IgM and IgG1 monoclonal antibodies raised against leukemic cells
Blixt O, Lavrova O, Mazurov D, Cló E, Kračun S, Bovin N, Filatov A. Analysis of Tn antigenicity with a panel of new IgM and IgG1 monoclonal antibodies raised against leukemic cells. Glycobiology 2011, 22: 529-542. PMID: 22143985, PMCID: PMC3287017, DOI: 10.1093/glycob/cwr178.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodies, Monoclonal, Murine-DerivedAntibody SpecificityAntigens, CDAntigens, Tumor-Associated, CarbohydrateBinding, CompetitiveHumansImmunoglobulin GImmunoglobulin MJurkat CellsLeukemiaMembrane GlycoproteinsMiceMolecular ChaperonesMolecular Sequence DataMucin-1Peptide FragmentsProtein BindingRecombinant ProteinsConceptsAnti-Tn mAbsPeptide contextTn antigenTerminal GalNAc residueHuman Jurkat T cellsJurkat T cellsIgG mAbsChaperone CosmcAmino acid serineT-synthaseAnti-TnPeptide motifsGalNAc residuesPolypeptide chainO-glycoproteinsIsoforms of CD45Jurkat cellsLeukemia diagnosticsAnti-Tn monoclonal antibodyMonoclonal antibodiesBinding analysisIgM mAbAntigenic structureCarbohydrate moietyMotif
2010
Quantitative Comparison of HTLV-1 and HIV-1 Cell-to-Cell Infection with New Replication Dependent Vectors
Mazurov D, Ilinskaya A, Heidecker G, Lloyd P, Derse D. Quantitative Comparison of HTLV-1 and HIV-1 Cell-to-Cell Infection with New Replication Dependent Vectors. PLOS Pathogens 2010, 6: e1000788. PMID: 20195464, PMCID: PMC2829072, DOI: 10.1371/journal.ppat.1000788.Peer-Reviewed Original ResearchConceptsCell-to-cell infectionEnv expression plasmidsHTLV-1 transmissionHIV-1HTLV-1Packaging vectorTarget cellsCocultured target cellsHIV-1 vectorsHIV-1 infectionHTLV-1 infectionCell-free infectionCell-cell interactionsReporter vectorInhibited HTLV-1Enhance virus infectionLymphocyte cell linesTarget cell typeHTLV-1 TaxEffector cellsReporter gene activityT cellsB cellsReporter gene expressionTime course study
2006
The Inner Loop of Tetraspanins CD82 and CD81 Mediates Interactions with Human T Cell Lymphotrophic Virus Type 1 Gag Protein*
Mazurov D, Heidecker G, Derse D. The Inner Loop of Tetraspanins CD82 and CD81 Mediates Interactions with Human T Cell Lymphotrophic Virus Type 1 Gag Protein*. Journal Of Biological Chemistry 2006, 282: 3896-3903. PMID: 17166843, DOI: 10.1074/jbc.m607322200.Peer-Reviewed Original ResearchConceptsTetraspanin-enriched microdomainsC-terminusN-terminusTetraspanin superfamily proteinsSite-directed mutationsMembrane protein complexesExtracellular loopCytoplasmic N-terminusHTLV-1T cell adhesionIntegrin functionPalmitoylated cysteinesSuperfamily proteinsProtein complexesTetraspanin CD82Cytoplasmic facePlasma membraneHTLV-1 GagColocalization approachesAmino acidsCD82IntegrinTetraspaninMutationsCD81
2005
HTLV-1 Gag protein associates with CD82 tetraspanin microdomains at the plasma membrane
Mazurov D, Heidecker G, Derse D. HTLV-1 Gag protein associates with CD82 tetraspanin microdomains at the plasma membrane. Virology 2005, 346: 194-204. PMID: 16325219, DOI: 10.1016/j.virol.2005.10.033.Peer-Reviewed Original ResearchConceptsHTLV-1 GagHTLV-1T cellsPresence of bacterial superantigensPlasma membraneGag proteinHIV-1 GagMembrane of T cellsAssociation of GagViral envelope glycoproteinsPlasma membrane of T cellsRaji B cellsCell extractsBacterial superantigensAntibody-mediated cross-linkingB cellsHIV-1Confocal image analysisEnvelope glycoproteinTetraspanin-enriched microdomainsJurkat T cellsImmunofluorescence stainingImmune synapseGag multimerizationIntercellular adhesion