2023
The RRE–Rev Module Has No Effect on the Packaging Efficiency of Cas9 and Gag Proteins into NanoMEDIC Virus-like Particles
Kruglova N, Komkov D, Mazurov D, Shepelev M. The RRE–Rev Module Has No Effect on the Packaging Efficiency of Cas9 and Gag Proteins into NanoMEDIC Virus-like Particles. Doklady Biological Sciences 2023, 513: s45-s50. PMID: 38472686, DOI: 10.1134/s0012496623700886.Peer-Reviewed Original ResearchMeSH KeywordsCRISPR-Cas SystemsGene Products, gagHIV-1HumansResponse ElementsRNA, Guide, CRISPR-Cas SystemsConceptsRev expression plasmidVirus-like particlesRev response elementEmpty control plasmidGene therapy of human diseasesGag proteinTherapy of human diseasesCas9 nucleaseGene therapyGenome editingGag expressionViral Gag proteinHIV Rev response elementTarget cellsEfficiency of genome editingMethods of genome editingControl plasmidProtein levelsNuclear exportNo effectPlasmid modificationGagResponse elementAccessory proteinsPlasmid constructs
2006
The Inner Loop of Tetraspanins CD82 and CD81 Mediates Interactions with Human T Cell Lymphotrophic Virus Type 1 Gag Protein*
Mazurov D, Heidecker G, Derse D. The Inner Loop of Tetraspanins CD82 and CD81 Mediates Interactions with Human T Cell Lymphotrophic Virus Type 1 Gag Protein*. Journal Of Biological Chemistry 2006, 282: 3896-3903. PMID: 17166843, DOI: 10.1074/jbc.m607322200.Peer-Reviewed Original ResearchConceptsTetraspanin-enriched microdomainsC-terminusN-terminusTetraspanin superfamily proteinsSite-directed mutationsMembrane protein complexesExtracellular loopCytoplasmic N-terminusHTLV-1T cell adhesionIntegrin functionPalmitoylated cysteinesSuperfamily proteinsProtein complexesTetraspanin CD82Cytoplasmic facePlasma membraneHTLV-1 GagColocalization approachesAmino acidsCD82IntegrinTetraspaninMutationsCD81
2005
HTLV-1 Gag protein associates with CD82 tetraspanin microdomains at the plasma membrane
Mazurov D, Heidecker G, Derse D. HTLV-1 Gag protein associates with CD82 tetraspanin microdomains at the plasma membrane. Virology 2005, 346: 194-204. PMID: 16325219, DOI: 10.1016/j.virol.2005.10.033.Peer-Reviewed Original ResearchConceptsHTLV-1 GagHTLV-1T cellsPresence of bacterial superantigensPlasma membraneGag proteinHIV-1 GagMembrane of T cellsAssociation of GagViral envelope glycoproteinsPlasma membrane of T cellsRaji B cellsCell extractsBacterial superantigensAntibody-mediated cross-linkingB cellsHIV-1Confocal image analysisEnvelope glycoproteinTetraspanin-enriched microdomainsJurkat T cellsImmunofluorescence stainingImmune synapseGag multimerizationIntercellular adhesion