2009
siRNA-based targeting of antiapoptotic genes can reverse chemoresistance in P-glycoprotein expressing chondrosarcoma cells
Kim DW, Kim KO, Shin MJ, Ha JH, Seo SW, Yang J, Lee FY. siRNA-based targeting of antiapoptotic genes can reverse chemoresistance in P-glycoprotein expressing chondrosarcoma cells. Molecular Cancer 2009, 8: 28. PMID: 19445670, PMCID: PMC2689171, DOI: 10.1186/1476-4598-8-28.Peer-Reviewed Original ResearchMeSH KeywordsAntineoplastic AgentsApoptosisATP Binding Cassette Transporter, Subfamily B, Member 1Bcl-X ProteinBone NeoplasmsCell Line, TumorChondrosarcomaDoxorubicinDrug Resistance, NeoplasmFlow CytometryGene SilencingGenes, bcl-2HumansImmunoblottingReverse Transcriptase Polymerase Chain ReactionRNA, Small InterferingX-Linked Inhibitor of Apoptosis ProteinConceptsAntiapoptotic proteinsChondrosarcoma cellsAntiapoptotic genesPharmacologic inhibitorsCell survival proteinsChondrosarcoma cell linesSurvival proteinsP-glycoproteinSiRNA knockdownCell typesProteinMechanisms of chemoresistanceCell linesGenesCommon mechanismChemoresistanceSiRNACellsP-glycoprotein inhibitionTherapeutic strategiesInhibitorsKnockdownApoptosisJJ012ConclusionThese findings
2007
ERK Signaling Regulates Macrophage Colony‐Stimulating Factor Expression Induced by Titanium Particles in MC3T3.E1 Murine Calvarial Preosteoblastic Cells
SEO SW, LEE D, CHO SK, KIM AD, MINEMATSU H, AYDEMIR A, GELLER JA, MACAULAY W, YANG J, LEE F. ERK Signaling Regulates Macrophage Colony‐Stimulating Factor Expression Induced by Titanium Particles in MC3T3.E1 Murine Calvarial Preosteoblastic Cells. Annals Of The New York Academy Of Sciences 2007, 1117: 151-158. PMID: 18056041, DOI: 10.1196/annals.1402.027.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell ProliferationCytokinesEnzyme InhibitorsExtracellular Signal-Regulated MAP KinasesGene Expression RegulationMacrophage Colony-Stimulating FactorMiceModels, BiologicalOsteoblastsRANK LigandReverse Transcriptase Polymerase Chain ReactionSignal TransductionSkullTitaniumConceptsM-CSF expressionExtracellular signal-regulated kinase (ERK) pathwaySignal-regulated kinase pathwayPreosteoblastic cellsSignal transduction pathwaysSpecific ERK inhibitorReverse transcriptase-polymerase chain reactionEnzyme-linked immunosorbent assayPreosteoclast proliferationTransduction pathwaysKinase pathwayMolecular mechanismsMacrophage colony-stimulating factorCellular environmentERK inhibitorPeriprosthetic osteolysisM-CSF productionNuclear factor kappa beta ligandCell populationsBeta ligandSignificant clinical problemTranscriptase-polymerase chain reactionExpressionDose-dependent mannerColony-stimulating factor
2006
Stromal cell‐derived factor 1 (SDF‐1, CXCL12) is increased in subacromial bursitis and downregulated by steroid and nonsteroidal anti‐inflammatory agents
Kim Y, Bigliani LU, Fujisawa M, Murakami K, Chang S, Lee H, Lee FY, Blaine TA. Stromal cell‐derived factor 1 (SDF‐1, CXCL12) is increased in subacromial bursitis and downregulated by steroid and nonsteroidal anti‐inflammatory agents. Journal Of Orthopaedic Research® 2006, 24: 1756-1764. PMID: 16779827, DOI: 10.1002/jor.20197.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAged, 80 and overAnti-Inflammatory AgentsAnti-Inflammatory Agents, Non-SteroidalBursa, SynovialBursitisCells, CulturedChemokine CXCL12Chemokines, CXCCyclooxygenase 2 InhibitorsDexamethasoneDown-RegulationEnzyme-Linked Immunosorbent AssayHumansIn Vitro TechniquesMiddle AgedOligonucleotide Array Sequence AnalysisReverse Transcriptase Polymerase Chain ReactionRNA, MessengerRotator CuffTendinopathyConceptsReal-time reverse transcription-polymerase chain reactionSDF-1 proteinEnzyme-linked immunosorbent assayCOX-2 inhibitorsStromal cell-derived factor-1Cell-derived factor-1SDF-1 expressionSubacromial bursaSDF-1Subacromial bursitisReal-time RT-PCRBursal cellsBursal tissueNormal bone marrow stromal cellsNonsteroidal anti-inflammatory agentsSDF-1 expression levelsAnti-inflammatory medicationsUse of steroidsAnti-inflammatory agentsFactor 1Reverse transcription-polymerase chain reactionSubacromial bursal tissueRotator cuff diseaseTranscription-polymerase chain reactionSubacromial bursa tissue
2005
Recruitment of osteoclast precursors by stromal cell derived factor‐1 (SDF‐1) in giant cell tumor of bone
Liao TS, Yurgelun MB, Chang S, Zhang H, Murakami K, Blaine TA, Parisian M, Kim W, Winchester RJ, Lee F. Recruitment of osteoclast precursors by stromal cell derived factor‐1 (SDF‐1) in giant cell tumor of bone. Journal Of Orthopaedic Research® 2005, 23: 203-209. PMID: 15607894, DOI: 10.1016/j.orthres.2004.06.018.Peer-Reviewed Original ResearchMeSH KeywordsBone NeoplasmsChemokine CXCL12Chemokines, CXCChemotaxis, LeukocyteGiant Cell Tumor of BoneHematopoietic Stem CellsHumansImmunohistochemistryMonocytesOsteoclastsReverse Transcriptase Polymerase Chain ReactionStem CellsConceptsGiant cell tumorSDF-1Osteoclast precursorsStromal cellsCell tumorsSDF-1 gene expressionTumor-induced osteoclastogenesisSDF-1 proteinRecombinant SDF-1Neoplastic stromal cellsReal-time RT-PCRHematopoetic stem cellsOsteoclast precursor cellsTime RT-PCRBone lesionsStudy of osteoclastogenesisChemotactic concentrationsChemoattractant factorGCT tissuesMonocyte/Specific receptorsRT-PCRDisease modelsPrecursor cellsFactor 1