2024
CRISPR-Cas9-based approaches for genetic analysis and epistatic interaction studies in Coxiella burnetii
Steiner S, Roy C. CRISPR-Cas9-based approaches for genetic analysis and epistatic interaction studies in Coxiella burnetii. MSphere 2024, 9: e00523-24. PMID: 39560384, PMCID: PMC11656778, DOI: 10.1128/msphere.00523-24.Peer-Reviewed Original ResearchIntracellular replicationHost cellsDouble mutantGenetic analysisDot/Icm type IVB secretion systemType IVB secretion systemConstruction of double mutantsLoss-of-function mutantsIntracellular replication defectsLysosome-derived vacuoleIVB secretion systemObligate intracellular bacterial pathogenRandom transposon mutagenesisTransposon insertion mutantsCRISPR-Cas9-based technologiesIntracellular bacterial pathogensLoss-of-function mutationsMammalian host cellsIntroduction of premature stop codonsPremature stop codonSilence gene expressionTargeted gene disruptionBase editing approachesEffector mutantsSecretion system
2022
Developmental Transitions Coordinate Assembly of the Coxiella burnetii Dot/Icm Type IV Secretion System
Park D, Steiner S, Shao M, Roy CR, Liu J. Developmental Transitions Coordinate Assembly of the Coxiella burnetii Dot/Icm Type IV Secretion System. Infection And Immunity 2022, 90: e00410-22. PMID: 36190257, PMCID: PMC9584302, DOI: 10.1128/iai.00410-22.Peer-Reviewed Original ResearchConceptsSmall cell variantLarge cell variantDot/Icm type IV secretion systemCell variantIntracellular replicationActive large cell variantHost cellsIntracellular bacterial pathogenType IV secretion systemDot/Icm T4SSObligate intracellular bacterial pathogenC. burnetiiCoxiella burnetiiBiphasic developmental cycleUnique biphasic developmental cycleInfectionNew host cellsSecretion systemBacterial pathogensInfectious formHost vacuoleBurnetiiMorphological changesCellsLater stages
2012
Nod1, but not the ASC inflammasome, contributes to induction of IL-1β secretion in human trophoblasts after sensing of Chlamydia trachomatis
Kavathas PB, Boeras CM, Mulla MJ, Abrahams VM. Nod1, but not the ASC inflammasome, contributes to induction of IL-1β secretion in human trophoblasts after sensing of Chlamydia trachomatis. Mucosal Immunology 2012, 6: 235-243. PMID: 22763410, PMCID: PMC3465624, DOI: 10.1038/mi.2012.63.Peer-Reviewed Original ResearchMeSH KeywordsCARD Signaling Adaptor ProteinsCarrier ProteinsCell LineChlamydia trachomatisCytoskeletal ProteinsGene ExpressionHumansInflammasomesInterleukin-1betaMyeloid Differentiation Factor 88NLR Family, Pyrin Domain-Containing 3 ProteinNod1 Signaling Adaptor ProteinToll-Like Receptor 2Toll-Like Receptor 4TrophoblastsConceptsIL-1β secretionToll-like receptorsCT infectionIL-1βASC inflammasomeChlamydia trachomatisKey Toll-like receptorsNalp3/ASC inflammasomePro-inflammatory cytokinesIL-1β mRNAIL-1β productionInnate immune pathwaysNOD-like receptorsHuman trophoblast cellsIntracellular bacterial pathogenChlamydia infectionImmune pathwaysObligate intracellular bacterial pathogenTrophoblast cellsHuman trophoblastsCaspase-1InfectionSecretionInflammasomeNOD1
2011
Generation of targeted Chlamydia trachomatis null mutants
Kari L, Goheen M, Randall L, Taylor L, Carlson J, Whitmire W, Virok D, Rajaram K, Endresz V, McClarty G, Nelson D, Caldwell H. Generation of targeted Chlamydia trachomatis null mutants. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108: 7189-7193. PMID: 21482792, PMCID: PMC3084044, DOI: 10.1073/pnas.1102229108.Peer-Reviewed Original ResearchConceptsGenetic systemBacterial pathogensObligate intracellular bacterial pathogenReverse-genetics approachTryptophan synthase genesEthyl methanesulfonate mutagenesisIntracellular bacterial pathogensGenome sequenceNull mutantsPCR ampliconsSynthase geneAnti-microbial effectsTarget genesMutantsChlamydial pathogenesisParental clonesMutationsTryptophan starvationGenomeInfected hundredsGenesPathogensSEXUALLY TRANSMITTED DISEASESMutagenic organicsTarget region
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