2023
Membrane tension induces F-actin reorganization and flow in a biomimetic model cortex
Sakamoto R, Banerjee D, Yadav V, Chen S, Gardel M, Sykes C, Banerjee S, Murrell M. Membrane tension induces F-actin reorganization and flow in a biomimetic model cortex. Communications Biology 2023, 6: 325. PMID: 36973388, PMCID: PMC10043271, DOI: 10.1038/s42003-023-04684-7.Peer-Reviewed Original ResearchConceptsF-actin reorganizationMechanical stressCell cortexBiochemical regulationCell shapeActomyosin cortexPhysical behaviorCell polarizationMembrane tensionCell migrationEssential physical behaviorPore openingSpatial assemblyDrive changesMembraneRelative rolesStressActinRegulationDiverse behaviorsRoleAssembly
2022
Fibroblasts secrete fibronectin under lamellipodia in a microtubule- and myosin II–dependent fashion
Huet-Calderwood C, Rivera-Molina F, Toomre D, Calderwood D. Fibroblasts secrete fibronectin under lamellipodia in a microtubule- and myosin II–dependent fashion. Journal Of Cell Biology 2022, 222: e202204100. PMID: 36416725, PMCID: PMC9699186, DOI: 10.1083/jcb.202204100.Peer-Reviewed Original ResearchConceptsFN secretionFocal adhesion dynamicsExtracellular matrixFocal adhesion formationSites of exocytosisLive-cell microscopyIntegrin-independent mannerCytoskeletal dynamicsFocal adhesionsAdhesion dynamicsRegulatory componentsMyosin IIIntact microtubulesCell polarizationCell adhesionIntegrin receptorsFN depositionLamellipodiaMicrotubulesFibronectinAdhesion formationNew adhesion formationFibroblastsII-dependent fashionCells
2017
A Cell Culture Model of Resistance Arteries.
Biwer L, Lechauve C, Vanhoose S, Weiss M, Isakson B. A Cell Culture Model of Resistance Arteries. Journal Of Visualized Experiments 2017 PMID: 28930992, PMCID: PMC5752193, DOI: 10.3791/55992.Peer-Reviewed Original ResearchConceptsCell culture modelCell typesMyoendothelial junctionsEndothelial cell polarizationPlating of cellsIntact cell layerDifferent cell typesSignaling microdomainCulture modelSpecific proteinsCell polarizationPhysiological relevanceActivity assaysProtein isolationSmooth muscle cellsVascular cellsMuscle cellsImmunofluorescent analysisIntact resistance arteriesProteinCellsMuscle fractionsIsolationCell layerVCCCMechanochemical feedback underlies coexistence of qualitatively distinct cell polarity patterns within diverse cell populations
Park J, Holmes WR, Lee SH, Kim HN, Kim DH, Kwak MK, Wang CJ, Edelstein-Keshet L, Levchenko A. Mechanochemical feedback underlies coexistence of qualitatively distinct cell polarity patterns within diverse cell populations. Proceedings Of The National Academy Of Sciences Of The United States Of America 2017, 114: e5750-e5759. PMID: 28655842, PMCID: PMC5514712, DOI: 10.1073/pnas.1700054114.Peer-Reviewed Original ResearchMeSH KeywordsCell Line, TumorCell MovementCell PolarityCell ShapeDisease ProgressionExtracellular MatrixFeedback, PhysiologicalGene Expression Regulation, NeoplasticHumansMelanomaModels, TheoreticalMutationNeoplasm InvasivenessOscillometryPhenotyperho GTP-Binding ProteinsSignal TransductionSkin NeoplasmsConceptsMechanochemical feedbackRho family small GTPasesDirectional cell migrationDiverse cell typesDiverse cell populationsUnderlying regulatory mechanismsPhenotypic plasticityCell polaritySmall GTPasesRegulatory mechanismsEnvironmental perturbationsPolarity patternCell polarizationPopulation variabilityCell migrationCell spreadCell typesExtracellular matrixMechanistic understandingCell contactCell populationsPhysiological conditionsMelanoma cellsDynamic patternsGTPases
2014
CS-31A NOVEL YAP-DRIVEN MIGRATION AND INVASION SIGNALING PATHWAY PREDICTS POOR OUTCOME IN GLIOBLASTOMA
Shah S, Tippens N, Park J, Mohyeldin A, Vela G, Levchenko A, Quinones-Hinojosa A. CS-31A NOVEL YAP-DRIVEN MIGRATION AND INVASION SIGNALING PATHWAY PREDICTS POOR OUTCOME IN GLIOBLASTOMA. Neuro-Oncology 2014, 16: v57-v58. PMCID: PMC4217989, DOI: 10.1093/neuonc/nou242.31.Peer-Reviewed Original ResearchCell migration pathwaysMechanism of YAPYAP-dependent mechanismsAbility of cellsDependent signaling mechanismRole of YAPFunction of YAPSmall Rho GTPaseExtracellular cuesTranscriptional regulatorsRho GTPaseSingle-cell mannerMolecular networksIntracellular signalingSignaling mechanismCell mannerCell polarizationCell migrationIndividual cellsCritical modulatorAggressive cancerYAPInvasive behaviorPoor patient prognosisYAP expressionSynergies between Aip1p and capping protein subunits (Acp1p and Acp2p) in clathrin-mediated endocytosis and cell polarization in fission yeast
Berro J, Pollard TD. Synergies between Aip1p and capping protein subunits (Acp1p and Acp2p) in clathrin-mediated endocytosis and cell polarization in fission yeast. Molecular Biology Of The Cell 2014, 25: 3515-3527. PMID: 25143407, PMCID: PMC4230613, DOI: 10.1091/mbc.e13-01-0005.Peer-Reviewed Original ResearchConceptsActin-depolymerizing factorActin filamentsFission yeastNew cellular functionsHeterodimeric capping proteinClathrin-mediated endocytosisActin filament barbedEndocytic patchesEndocytic sitesCellular functionsAip1pCapping proteinEndocytic vesiclesFilament barbedCapping activityPlasma membraneProtein subunitsActin meshworkCell polarizationIndependent pathwaysProteinEndocytosisYeastQuantitative microscopyFilaments
2013
Live-cell imaging of exocyst links its spatiotemporal dynamics to various stages of vesicle fusion
Rivera-Molina F, Toomre D. Live-cell imaging of exocyst links its spatiotemporal dynamics to various stages of vesicle fusion. Journal Of Cell Biology 2013, 201: 673-680. PMID: 23690179, PMCID: PMC3664709, DOI: 10.1083/jcb.201212103.Peer-Reviewed Original ResearchConceptsLive-cell imagingVesicle fusionEndocytic recycling compartmentAttachment protein receptorsSNARE fusion machineryExocyst complexMembrane traffickingFusion machineryRecycling compartmentCell cortexSec8Cell protrusionsPlasma membraneVesicle attachmentFusion poreMembrane expansionProtein receptorsCell polarizationFluorescence recoverySpatiotemporal dynamicsExocystUbiquitous roleVesiclesTraffickingMorphological criteria
2010
Deciphering subcellular processes in live imaging datasets via dynamic probabilistic networks
Letinic K, Sebastian R, Barthel A, Toomre D. Deciphering subcellular processes in live imaging datasets via dynamic probabilistic networks. Bioinformatics 2010, 26: 2029-2036. PMID: 20581401, PMCID: PMC2916721, DOI: 10.1093/bioinformatics/btq331.Peer-Reviewed Original ResearchConceptsTotal internal reflection fluorescence microscopyCellular statesDistinct cellular statesSpatial-temporal regulationNon-polarized cellsComplex intracellular processesReflection fluorescence microscopyLive cell imaging dataOrganelle behaviorGLUT4 vesiclesProtein complexesCellular processesSpatial regulationDevelopmental processesBiological processesSubcellular processesCell imaging dataCell polarizationLiving cellsIntracellular processesBlood glucose homeostasisFluorescence microscopyExocytosisCell imagingStatic snapshots
2008
Epithelial junctions and polarity: complexes and kinases
Caplan MJ, Seo-Mayer P, Zhang L. Epithelial junctions and polarity: complexes and kinases. Current Opinion In Nephrology & Hypertension 2008, 17: 506-512. PMID: 18695392, PMCID: PMC3057677, DOI: 10.1097/mnh.0b013e32830baaae.Peer-Reviewed Original ResearchConceptsEpithelial cell polarityCell polarityEpithelial cell polarizationJunctional complexesIntercellular adhesion junctionsImportant physiological ramificationsCalcium-dependent adhesion proteinCell-cell contactCellular energy metabolismProtein complexesMembrane proteinsAdhesion junctionsPlasmalemmal domainsAdhesion proteinsCell polarizationPhysiological ramificationsEpithelial junctionsKinaseEnergy metabolismCharacteristic polarityEpithelial cellsProteinParacellular permeabilityNovel classComplexes
2006
Syndecan-4 Clustering Induces Cell Migration in a PDZ-Dependent Manner
Tkachenko E, Elfenbein A, Tirziu D, Simons M. Syndecan-4 Clustering Induces Cell Migration in a PDZ-Dependent Manner. Circulation Research 2006, 98: 1398-1404. PMID: 16675718, DOI: 10.1161/01.res.0000225283.71490.5a.Peer-Reviewed Original ResearchConceptsCell migrationPDZ-dependent mannerAdhesion pointsActivation of Rac1Induces cell migrationPDZ domainEndothelial cell migrationFocal adhesionsCytoplasmic domainSignal transductionCell motilityDomain interactionsEndothelial cellsSyndecan-4Cell polarizationPDZRac1Fide receptorLigand clusteringLive cellsDirection of migrationElevated basal levelsSynectinS4 mutantLeading edge
2005
Integrinα5 and Delta/Notch Signaling Have Complementary Spatiotemporal Requirements during Zebrafish Somitogenesis
Ju¨lich D, Geisler R, Consortium T, Holley S. Integrinα5 and Delta/Notch Signaling Have Complementary Spatiotemporal Requirements during Zebrafish Somitogenesis. Developmental Cell 2005, 8: 575-586. PMID: 15809039, DOI: 10.1016/j.devcel.2005.01.016.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAnimals, Genetically ModifiedBody PatterningCell PolarityExtracellular MatrixFibronectinsGene Expression Regulation, DevelopmentalIn Situ HybridizationIntegrin alpha5Intracellular Signaling Peptides and ProteinsMembrane ProteinsMolecular Sequence DataMorphogenesisPhenotypePoint MutationReceptors, NotchRecombinant Fusion ProteinsSignal TransductionSomitesZebrafishZebrafish ProteinsConceptsFibronectin matrix assemblyDelta/Notch signalingMatrix assemblyNotch pathway mutantsEntire body axisZebrafish somitogenesisVertebrate embryogenesisPathway mutantsDouble mutantSomite defectsSomite formationPosterior somitesGenetic controlSomitogenesisNotch signalingNotch pathwayEpithelial transitionSpatiotemporal requirementsBody axisCell polarizationSegmentation defectsPoint mutationsMutantsComplete lossSkeletal muscle
2002
The Identification of CVP1 Reveals a Role for Sterols in Vascular Patterning
Carland F, Fujioka S, Takatsuto S, Yoshida S, Nelson T. The Identification of CVP1 Reveals a Role for Sterols in Vascular Patterning. The Plant Cell 2002, 14: 2045-2058. PMID: 12215504, PMCID: PMC150754, DOI: 10.1105/tpc.003939.Peer-Reviewed Original ResearchMeSH KeywordsAllelesArabidopsisArabidopsis ProteinsBiological TransportBrassinosteroidsCholestanolsCloning, MolecularCotyledonGene Expression Regulation, DevelopmentalGene Expression Regulation, PlantIn Situ HybridizationMethyltransferasesMutationPhytosterolsPlants, Genetically ModifiedRNA, MessengerSignal TransductionSteroids, HeterocyclicTriazolesConceptsSterol biosynthetic pathwayBrassinosteroid levelsCell polarityOrgan expansionVascular patterningAllelic seriesBiosynthetic pathwayCell polarizationVascular strandsAdditional functionsEnzymatic activityVascular cellsSterolsPathwayCvp1Branch pointsMutantsGenesRoleMutationsEnzymePatterningCellsStrandsElongation
2001
Increased filamin binding to β-integrin cytoplasmic domains inhibits cell migration
Calderwood D, Huttenlocher A, Kiosses W, Rose D, Woodside D, Schwartz M, Ginsberg M. Increased filamin binding to β-integrin cytoplasmic domains inhibits cell migration. Nature Cell Biology 2001, 3: 1060-1068. PMID: 11781567, DOI: 10.1038/ncb1201-1060.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SubstitutionAnimalsBinding SitesCell MovementCell PolarityCHO CellsContractile ProteinsCricetinaeCytoplasmCytoskeletonFibronectinsFilaminsFocal AdhesionsHumansIntegrin beta ChainsIntegrinsIsoleucineJurkat CellsMicrofilament ProteinsProtein Structure, TertiaryRecombinant Fusion ProteinsTalinValineConceptsFocal adhesion formationFilamin bindingCell migrationMembrane protrusionsMatrix assemblyIntegrin-dependent cell migrationFibronectin matrix assemblyAmino acid substitutionsInhibits cell migrationAnimal developmentActin cytoskeletonIntegrin tailsBiochemical signalsAdhesion receptorsFilaminCell polarizationTalinAcid substitutionsExtracellular matrixAdhesion formationTailBindingAssemblyMigrationSelective loss
2000
Compartmentalization of the Cell Cortex by Septins Is Required for Maintenance of Cell Polarity in Yeast
Barral Y, Mermall V, Mooseker M, Snyder M. Compartmentalization of the Cell Cortex by Septins Is Required for Maintenance of Cell Polarity in Yeast. Molecular Cell 2000, 5: 841-851. PMID: 10882120, DOI: 10.1016/s1097-2765(00)80324-x.Peer-Reviewed Original ResearchMeSH KeywordsActinsCarrier ProteinsCell CompartmentationCell Cycle ProteinsCell DivisionCell MembraneCell PolarityCytoplasmCytoskeletal ProteinsExocytosisFungal ProteinsMorphogenesisMyosin Heavy ChainsMyosin Type IIMyosin Type VProtein-Tyrosine KinasesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSchizosaccharomyces pombe ProteinsConceptsCell polaritySpecialized plasma membrane domainsIsotropic bud growthPlasma membrane domainsBud neckMembrane domainsMother cellsCell cortexCell peripheryGrowth polaritySeptinsProper regulationBud surfaceBiological processesBud growthCell polarizationIsotropic growthCortical domainsExocytosisPatch stabilityActive siteCellsMyo2Sec5SPA2
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