2020
RPE Polarity and Barrier Function
Rizzolo L. RPE Polarity and Barrier Function. 2020, 19-45. DOI: 10.1007/978-3-030-28384-1_2.ChaptersCell polarityEpithelial cell polarityPlasma membrane domainsTight junctionsBasolateral plasma membraneRPE polarityCellular processesMembrane domainsPlasma membraneMembrane transportersMembrane transportNeighboring cellsRPE phenotypeSemi-selective barrierProteinBarrier functionJunction proteinsRPE functionParacellular spaceOuter blood-retinal barrierVisual cycleTight junction proteinsTransepithelial electrical resistanceAsymmetric distributionDynamic interplay
2015
Akt Substrate of 160 kD Regulates Na+,K+-ATPase Trafficking in Response to Energy Depletion and Renal Ischemia
Alves DS, Thulin G, Loffing J, Kashgarian M, Caplan MJ. Akt Substrate of 160 kD Regulates Na+,K+-ATPase Trafficking in Response to Energy Depletion and Renal Ischemia. Journal Of The American Society Of Nephrology 2015, 26: 2765-2776. PMID: 25788531, PMCID: PMC4625659, DOI: 10.1681/asn.2013101040.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiotinylationCell LineCytoplasmDogsDynaminsEndocytosisEpithelial CellsGTPase-Activating ProteinsHumansIschemiaKidneyKidney DiseasesMadin Darby Canine Kidney CellsMaleMiceMice, KnockoutMicroscopy, FluorescencePhosphorylationProtein TransportReperfusion InjuryRNA, Small InterferingSignal TransductionSodium-Potassium-Exchanging ATPaseConceptsRenal epithelial cellsATPase traffickingIntracellular compartmentsEpithelial cell polarityEpithelial cellsBasolateral plasma membraneGlucose transporter 4Cultured epithelial cellsCell polarityRab GTPaseAkt substratePlasma membraneSubcellular distributionAS160Energy depletionDirect bindingTransporter 4TraffickingDirect roleK-ATPaseATPaseTubular soluteIntracellular accumulationCellsCompartments
2012
Cell polarity and cystic kidney disease
Fedeles S, Gallagher AR. Cell polarity and cystic kidney disease. Pediatric Nephrology 2012, 28: 1161-1172. PMID: 23161205, DOI: 10.1007/s00467-012-2337-z.Peer-Reviewed Original ResearchConceptsPlanar cell polarityCell polarityApical-basolateral polarityEpithelial cell polarityConserved Protein ComplexesCystic kidney diseasePolarity mechanismsProtein complexesOrgan developmentIntriguing crosstalkMolecular processesRecent findingsPolycystic kidney diseasePolarityInteresting questionsCrosstalkComplexesMaintenance
2011
Preactivation of AMPK by metformin may ameliorate the epithelial cell damage caused by renal ischemia
Seo-Mayer PW, Thulin G, Zhang L, Alves DS, Ardito T, Kashgarian M, Caplan MJ. Preactivation of AMPK by metformin may ameliorate the epithelial cell damage caused by renal ischemia. American Journal Of Physiology. Renal Physiology 2011, 301: f1346-f1357. PMID: 21849490, PMCID: PMC3233870, DOI: 10.1152/ajprenal.00420.2010.Peer-Reviewed Original ResearchConceptsEpithelial cell polarityMDCK cellsPlasma membrane domainsIon transport proteinsEpithelial cell organizationCellular energy sensorAMPK activator metforminMadin-Darby canine kidney cellsBasolateral plasma membraneShort hairpin RNACanine kidney cellsCell polarityImmunofluoresence localizationRenal epithelial cellsMembrane domainsNa-K-ATPaseProtein kinaseAMPK activatorPlasma membraneVesicular compartmentsAMPK activityTransport proteinsEnergy sensorMolecular consequencesBasolateral localization
2010
Lymphocytes Accelerate Epithelial Tight Junction Assembly: Role of AMP-Activated Protein Kinase (AMPK)
Tang XX, Chen H, Yu S, Zhang L, Caplan MJ, Chan HC. Lymphocytes Accelerate Epithelial Tight Junction Assembly: Role of AMP-Activated Protein Kinase (AMPK). PLOS ONE 2010, 5: e12343. PMID: 20808811, PMCID: PMC2925955, DOI: 10.1371/journal.pone.0012343.Peer-Reviewed Original ResearchConceptsTJ assemblyActivation of AMPKProtein kinaseEpithelial cellsCalcium switch experimentsEpithelial cell polaritySuppression of AMPKTight junction assemblyRole of AMPMadin-Darby canine kidney cellsCellular ATP levelsCanine kidney cellsTight junctionsCell polarityApicolateral borderJunction assemblyModel cell lineAMPK activationProper formationAdjacent epithelial cellsTJ formationChemical inhibitorsCalu-3 human airway epithelial cellsHuman airway epithelial cellsMDCK cells
2008
Epithelial junctions and polarity: complexes and kinases
Caplan MJ, Seo-Mayer P, Zhang L. Epithelial junctions and polarity: complexes and kinases. Current Opinion In Nephrology & Hypertension 2008, 17: 506-512. PMID: 18695392, PMCID: PMC3057677, DOI: 10.1097/mnh.0b013e32830baaae.Peer-Reviewed Original ResearchConceptsEpithelial cell polarityCell polarityEpithelial cell polarizationJunctional complexesIntercellular adhesion junctionsImportant physiological ramificationsCalcium-dependent adhesion proteinCell-cell contactCellular energy metabolismProtein complexesMembrane proteinsAdhesion junctionsPlasmalemmal domainsAdhesion proteinsCell polarizationPhysiological ramificationsEpithelial junctionsKinaseEnergy metabolismCharacteristic polarityEpithelial cellsProteinParacellular permeabilityNovel classComplexes
2003
Histopathological analysis of renal cystic epithelia in the Pkd2WS25/- mouse model of ADPKD
Thomson RB, Mentone S, Kim R, Earle K, Delpire E, Somlo S, Aronson PS. Histopathological analysis of renal cystic epithelia in the Pkd2WS25/- mouse model of ADPKD. American Journal Of Physiology. Renal Physiology 2003, 285: f870-f880. PMID: 12851251, DOI: 10.1152/ajprenal.00153.2003.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAnimalsCadherinsDisease Models, AnimalEpitheliumGene SilencingImmunohistochemistryKidneyMiceMicroscopy, ElectronMicroscopy, Electron, ScanningPolycystic Kidney, Autosomal DominantProtein Kinase D2Protein KinasesSodium-Potassium-Chloride SymportersSodium-Potassium-Exchanging ATPaseSolute Carrier Family 12, Member 2Tissue DistributionConceptsCell polarityKsp-cadherinBasolateral membraneRenal cystic epitheliaEpithelial cell polarityEpithelial cellsSpecific membrane proteinsCell dedifferentiationEpithelial cell dedifferentiationE-cadherinRenal epithelial cellsCystic epithelial cellsMembrane proteinsPhenotypic transitionCl(-) cotransporter NKCC1Expression levelsATPaseSecretory isoformEarly cystsTransepithelial secretionImmunocytochemical analysisUltrastructural analysisAutosomal dominant polycystic kidney diseaseDominant polycystic kidney diseasePolycystic kidney disease
1997
Epithelial Cell Polarity: Challenges and Methodologies
Caplan M, Rodriguez‐Boulan E. Epithelial Cell Polarity: Challenges and Methodologies. 1997, 665-688. DOI: 10.1002/cphy.cp140117.Peer-Reviewed Original ResearchEpithelial polarityProtein trafficking pathwaysEpithelial cell polarityTissue-specific variationsEpithelial cellsSorting machineryProtein polarityCell polaritySorting signalsTargeting pathwaysTrafficking pathwaysRecycling pathwayBiochemical techniquesE-cadherinVitro systemEpithelial monolayersGenetic modelsPathwayBiogenetic pathwayMorphological techniquesCellsPolarityMachinerySubstrataTranscytosis
1995
Alpha 1(E)-catenin is an actin-binding and -bundling protein mediating the attachment of F-actin to the membrane adhesion complex.
Rimm DL, Koslov ER, Kebriaei P, Cianci CD, Morrow JS. Alpha 1(E)-catenin is an actin-binding and -bundling protein mediating the attachment of F-actin to the membrane adhesion complex. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 8813-8817. PMID: 7568023, PMCID: PMC41057, DOI: 10.1073/pnas.92.19.8813.Peer-Reviewed Original ResearchConceptsF-actinBundling proteinE-cadherin-mediated cell-cell contactsHomotypic cell-cell adhesionBundles F-actinEpithelial cell polarityCortical actin cytoskeletonCell-cell adhesionActin-binding proteinsFull-length proteinE-cadherinCell-cell contactMembrane adhesion complexesBundles actinCell polarityHierarchy of interactionsActin cytoskeletonAdhesion complexesCytoplasmic domainCosedimentation assaysSedimentation assaysAdditional proteinsMolecular basisActin filamentsActin complex
1988
Development of Secretagogue Responsiveness in the Pancreas
Jamieson JD, Gorelick FS, Chang A. Development of Secretagogue Responsiveness in the Pancreas. Scandinavian Journal Of Gastroenterology 1988, 23: 98-103. PMID: 2852401, DOI: 10.3109/00365528809095920.Peer-Reviewed Original ResearchConceptsSecretory pathwaySecretory proteinsPancreatic acinar cellsEpithelial cell polarityDependent protein kinase IIRegulated secretory pathwayConstitutive secretory pathwayAcinar cellsProtein kinase IICell polarityMembrane proteinsPhosphorylated substratesRegulated secretionBasolateral domainCell biologyPlasmalemmal domainsPlasma membraneKinase IISecond messengerSecretagogue responsivenessFunctional specializationProteinEpithelial cellsPathwayZymogen granules
1986
Secretion of endogenous and exogenous proteins from polarized MDCK cell monolayers.
Gottlieb T, Beaudry G, Rizzolo L, Colman A, Rindler M, Adesnik M, Sabatini D. Secretion of endogenous and exogenous proteins from polarized MDCK cell monolayers. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 2100-2104. PMID: 3083413, PMCID: PMC323238, DOI: 10.1073/pnas.83.7.2100.Peer-Reviewed Original ResearchConceptsMDCK cellsCellular endomembrane systemEpithelial cell polarityMembrane-associated precursorEndogenous productsMature secretory productCell polarityPolarized MDCK cell monolayersEndomembrane systemAlpha 2u-globulinCorresponding genesSecretory pathwayMDCK cell monolayersRegulated pathwaySecretory productsImmunoglobulin kappa chainConstitutive secretionExogenous proteinsProteolytic cleavageSorting mechanismBasolateral surfaceApical surfaceProteinVectorial secretionCell of origin
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