2023
Bioactive signalling lipids as drivers of chronic liver diseases
Kaffe E, Tisi A, Magkrioti C, Aidinis V, Mehal W, Flavell R, Maccarrone M. Bioactive signalling lipids as drivers of chronic liver diseases. Journal Of Hepatology 2023, 80: 140-154. PMID: 37741346, DOI: 10.1016/j.jhep.2023.08.029.Peer-Reviewed Original ResearchConceptsChronic liver diseaseLiver diseasePrevalent chronic liver diseaseBioactive lipidsPotential therapeutic targetG protein-coupled receptorsProtein-coupled receptorsTherapeutic targetPoly-unsaturated fatty acidsMalignant transformationPotent modulatorEnergy homeostasisDiseaseCell proliferationSignaling lipidsTissue repairReceptorsFatty acidsMultiple cellular functionsLipidsBioactive roleBioactive signaling lipidsInflammationProgressionLipid remodeling in megakaryocyte differentiation and platelet biogenesis
Jain K, Tyagi T, Hwa J. Lipid remodeling in megakaryocyte differentiation and platelet biogenesis. Nature Cardiovascular Research 2023, 2: 803-804. PMID: 37736249, PMCID: PMC10512809, DOI: 10.1038/s44161-023-00324-9.Commentaries, Editorials and LettersRBG Motif Bridge-Like Lipid Transport Proteins: Structure, Functions, and Open Questions
Hanna M, Guillén-Samander A, De Camilli P. RBG Motif Bridge-Like Lipid Transport Proteins: Structure, Functions, and Open Questions. Annual Review Of Cell And Developmental Biology 2023, 39: 409-434. PMID: 37406299, DOI: 10.1146/annurev-cellbio-120420-014634.Peer-Reviewed Original ResearchLipid transfer proteinMembrane contact sitesVesicle-mediated trafficTransport of lipidsPutative physiological roleEukaryotic cellsEndocytic pathwayContact sitesLipid transportPhysiological roleTransfer proteinProteinHydrophobic channelRod-like structureLipidsEntire lengthDevelopmental disordersCytosolMutationsNew familyTransportPathwayMechanismMembraneCellsATG9 vesicles comprise the seed membrane of mammalian autophagosomes
Olivas T, Wu Y, Yu S, Luan L, Choi P, Guinn E, Nag S, De Camilli P, Gupta K, Melia T. ATG9 vesicles comprise the seed membrane of mammalian autophagosomes. Journal Of Cell Biology 2023, 222: e202208088. PMID: 37115958, PMCID: PMC10148236, DOI: 10.1083/jcb.202208088.Peer-Reviewed Original ResearchConceptsAtg9 vesiclesMammalian autophagosomesStyrene maleic acid lipid particlesLipid scramblase activityLC3-IIAutophagosomes formAutophagosome membraneMature autophagosomesScramblase activityAutophagosome formationAtg9Lipid transportMembrane growthAutophagosomesNanoscale organizationProtein-mediated transferProteinMembrane surface areaOrganellesVesiclesSeed membraneMembraneLipid particlesLipidsDifferent stagesDirect determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer
Panda A, Giska F, Duncan A, Welch A, Brown C, McAllister R, Hariharan P, Goder J, Coleman J, Ramakrishnan S, Pincet F, Guan L, Krishnakumar S, Rothman J, Gupta K. Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer. Nature Methods 2023, 20: 891-897. PMID: 37106230, PMCID: PMC10932606, DOI: 10.1038/s41592-023-01864-5.Peer-Reviewed Original ResearchConceptsIntegral membrane proteinsMembrane proteinsOligomeric organizationOligomeric stateNative mass spectrometry analysisFunctional oligomeric stateKey membrane componentMass spectrometry analysisNMS analysisTarget membraneLipid bindingMembrane componentsProteolipid vesiclesMembrane compositionLipid compositionSpectrometry analysisLipid membranesNeurotransmitter releaseProteinMembraneLipidsMembrane propertiesDirect determinationBilayersTransportersSpatiotemporal Heterogeneity of De Novo Lipogenesis in Fixed and Living Single Cells
Shuster S, Burke M, Davis C. Spatiotemporal Heterogeneity of De Novo Lipogenesis in Fixed and Living Single Cells. The Journal Of Physical Chemistry B 2023, 127: 2918-2926. PMID: 36976708, PMCID: PMC12203757, DOI: 10.1021/acs.jpcb.2c08812.Peer-Reviewed Original ResearchConceptsDe novo lipogenesisPanc1 pancreatic cancer cellsPancreatic cancer cellsLipid dropletsType II diabetesGlucose metabolismNovo lipogenesisII diabetesLipid droplet morphologyLiver tissueGlucose uptakeCancer cellsAdipocyte cellsHigh rateLipogenesisDNL ratesCritical metabolic processesAdipocytesMajority of lipidsCellsLipidsBetter preservationObesityDiabetesTriglyceridesSeparating Inner and Outer Membranes of Escherichia coli by EDTA-free Sucrose Gradient Centrifugation
Shu S, Mi W. Separating Inner and Outer Membranes of Escherichia coli by EDTA-free Sucrose Gradient Centrifugation. Bio-protocol 2023, 13: e4638. PMID: 36968434, PMCID: PMC10031520, DOI: 10.21769/bioprotoc.4638.Peer-Reviewed Original ResearchInner membraneOuter membraneGram-negative bacteriaPeptidoglycan cell wallEscherichia coliMembrane protein purificationTotal cell membranesSucrose gradient centrifugationMembrane proteinsCell wallProtein structureFunctional studiesProtein purificationTotal membranesCell membraneSucrose gradientsBiochemical proceduresGradient centrifugationProteinMembraneColiBacteriaGradient ultracentrifugationLipidsUltracentrifugation method
2022
Mitoguardin-2–mediated lipid transfer preserves mitochondrial morphology and lipid droplet formation
Hong Z, Adlakha J, Wan N, Guinn E, Giska F, Gupta K, Melia TJ, Reinisch KM. Mitoguardin-2–mediated lipid transfer preserves mitochondrial morphology and lipid droplet formation. Journal Of Cell Biology 2022, 221: e202207022. PMID: 36282247, PMCID: PMC9597353, DOI: 10.1083/jcb.202207022.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulumLipid dropletsProtein-mediated transferLipid transport proteinsLipid droplet formationLD biologyMitochondrial proteinsSecretory pathwayMass spectrometry analysisTerminal domainMitochondrial morphologyTransport proteinsLipid transportersCellular membranesLD metabolismMembrane contactX-ray structureSpectrometry analysisOrganellesGlycerophospholipidsProteinHydrophobic cavityFatty acidsLipidsMembraneUse of host lipids by the Lyme disease spirochete may lead to biomarkers
Arora G, Hart T, Fikrig E. Use of host lipids by the Lyme disease spirochete may lead to biomarkers. Journal Of Clinical Investigation 2022, 132: e158254. PMID: 35289311, PMCID: PMC8920323, DOI: 10.1172/jci158254.Peer-Reviewed Original ResearchConceptsB. burgdorferi infectionLyme diseaseBurgdorferi infectionCareful clinical assessmentCommon tick-borne diseaseAnti-lipid antibodiesProduction of antibodiesTick-borne diseaseHost lipidsAntibody titersLyme disease agent Borrelia burgdorferiClinical assessmentCurrent biomarkersMurine modelDiseaseSerum samplesLyme disease spirocheteAntibodiesBorrelia burgdorferiInfectionBiomarkersPatientsLipidsSyphilisPhosphatidic acidBioactive lipids and metabolic syndrome—a symposium report
DeVito LM, Dennis EA, Kahn BB, Shulman GI, Witztum JL, Sadhu S, Nickels J, Spite M, Smyth S, Spiegel S. Bioactive lipids and metabolic syndrome—a symposium report. Annals Of The New York Academy Of Sciences 2022, 1511: 87-106. PMID: 35218041, PMCID: PMC9219555, DOI: 10.1111/nyas.14752.Peer-Reviewed Original ResearchConceptsBioactive lipidsMetabolic syndromeCardiometabolic conditionsCardiovascular diseaseAnimal modelsDietary lipidsLipid metabolismMetabolic homeostasisMultitude of functionsLipidomic approachLipid pathwaysContinued investigationSyndromeMolecular functionsSymposium reportGenetic studiesLipidsPathwayInflammationGreater understandingDiseaseLiverMacrophagesLipogenesisA tunable lipid bilayer native MS platform for direct determination of hierarchical organization of membrane proteins and lipids at the membrane
Panda A, Giska F, Brown C, Coleman J, Rothman J, Gupta K. A tunable lipid bilayer native MS platform for direct determination of hierarchical organization of membrane proteins and lipids at the membrane. Biophysical Journal 2022, 121: 312a-313a. DOI: 10.1016/j.bpj.2021.11.1192.Peer-Reviewed Original ResearchQuantitative Models of Lipid Transfer and Membrane Contact Formation
Zhang Y, Ge J, Bian X, Kumar A. Quantitative Models of Lipid Transfer and Membrane Contact Formation. Contact 2022, 5: 25152564221096024. PMID: 36120532, PMCID: PMC9481209, DOI: 10.1177/25152564221096024.Peer-Reviewed Original ResearchMembrane contact sitesLipid transfer proteinLipid transferMembrane tensionMCS formationLipid exchange mechanismsMembrane contact formationOrganelle biogenesisExtended synaptotagminsOrganelle dynamicsMembrane proteinsDifferent organellesMembrane bindingMembrane expansionContact sitesMolecular mechanismsLipid flowLipid homeostasisTransfer proteinSimple lipidsProteinMembraneDistinct compositionLipidsKey role
2021
Coupling lipid synthesis with nuclear envelope remodeling
Barger SR, Penfield L, Bahmanyar S. Coupling lipid synthesis with nuclear envelope remodeling. Trends In Biochemical Sciences 2021, 47: 52-65. PMID: 34556392, PMCID: PMC9943564, DOI: 10.1016/j.tibs.2021.08.009.Peer-Reviewed Original ResearchConceptsNuclear envelopeEndoplasmic reticulumNuclear membraneNuclear envelope remodelingLipid-protein interactionsBiosynthesis of lipidsNE remodelingGenome protectionDynamic remodeling processesNE dynamicsLipid speciesCell growthBilayer lipidsLipid synthesisNew roleMembraneLipidsRecent evidenceRemodeling processGenomeRemodelingBiosynthesisProtective barrierSpeciesProteinInsights into VPS13 properties and function reveal a new mechanism of eukaryotic lipid transport
Leonzino M, Reinisch KM, De Camilli P. Insights into VPS13 properties and function reveal a new mechanism of eukaryotic lipid transport. Biochimica Et Biophysica Acta (BBA) - Molecular And Cell Biology Of Lipids 2021, 1866: 159003. PMID: 34216812, PMCID: PMC8325632, DOI: 10.1016/j.bbalip.2021.159003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutophagosomesAutophagy-Related ProteinsCryoelectron MicroscopyDisease Models, AnimalEukaryotic CellsHeredodegenerative Disorders, Nervous SystemHumansHydrophobic and Hydrophilic InteractionsLipid BilayersLipid MetabolismMitochondrial MembranesMutationProtein DomainsStructure-Activity RelationshipVesicular Transport ProteinsYeastsConceptsLipid transportMembrane contact sitesDomain protein familyOccurrence of proteinsVPS13 proteinsEukaryotic cellsNumerous proteinsProtein familyIntracellular membranesProtein bridgeHydrophobic grooveContact sitesMembrane growthLipid transferBilayer lipidsNovel mechanismVps13New mechanismProteinLipidsAtg2OrganellesAdjacent bilayersDiscoveryMechanismDietary lipids as regulators of reward processes: multimodal integration matters
Berland C, Small DM, Luquet S, Gangarossa G. Dietary lipids as regulators of reward processes: multimodal integration matters. Trends In Endocrinology And Metabolism 2021, 32: 693-705. PMID: 34148784, DOI: 10.1016/j.tem.2021.05.008.Peer-Reviewed Original ResearchConceptsDietary lipidsFunctional modulatorsModern food environmentLipid sensingPalatable dietObesity pandemicDopamine transmissionDA circuitsFeeding behaviorBody homeostasisDA signalingReward circuitRecent findingsDA systemEnergy-related signalsGenetic conditionsFood environmentFood overconsumptionNeural substratesLipidsRecent reportsSignalingRegulatorHomeostasisReward processesCD1a selectively captures endogenous cellular lipids that broadly block T cell response
Cotton R, Wegrecki M, Cheng T, Chen Y, Veerapen N, Le Nours J, Orgill D, Pomahac B, Talbot S, Willis R, Altman J, de Jong A, Van Rhijn I, Clark R, Besra G, Ogg G, Rossjohn J, Moody D. CD1a selectively captures endogenous cellular lipids that broadly block T cell response. Journal Of Experimental Medicine 2021, 218: e20202699. PMID: 33961028, PMCID: PMC8111460, DOI: 10.1084/jem.20202699.Peer-Reviewed Original ResearchConceptsNatural endogenous inhibitorsEndogenous lipidsCD1a proteinCellular lipidsT cell activationMembrane phospholipidsLonger lipidsSurface residuesEndogenous inhibitorLipidomics methodDetailed chemical structureCell activationLipidsCellsNatural blockerSphingolipidsDisplay platformProteinCrystal structureResiduesCell responsesBindingTetramerC42SphingomyelinA model for a partnership of lipid transfer proteins and scramblases in membrane expansion and organelle biogenesis
Ghanbarpour A, Valverde DP, Melia TJ, Reinisch KM. A model for a partnership of lipid transfer proteins and scramblases in membrane expansion and organelle biogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2101562118. PMID: 33850023, PMCID: PMC8072408, DOI: 10.1073/pnas.2101562118.Peer-Reviewed Original ResearchConceptsLipid transfer proteinEndoplasmic reticulumAutophagy protein ATG2Membrane dynamics processesTransfer proteinOrganelle biogenesisAutophagosome biogenesisCytosolic leafletOrganelle membranesMembrane expansionScramblasesVMP1Lipid homeostasisTMEM41BAtg9BiogenesisBulk lipidsProteinLipidsAtg2GolgiOrganellesReticulumLeafletsHomeostasisHuman skin is colonized by T cells that recognize CD1a independently of lipid
Cotton R, Cheng T, Wegrecki M, Le Nours J, Orgill D, Pomahac B, Talbot S, Willis R, Altman J, de Jong A, Ogg G, Van Rhijn I, Rossjohn J, Clark R, Moody D. Human skin is colonized by T cells that recognize CD1a independently of lipid. Journal Of Clinical Investigation 2021, 131: e140706. PMID: 33393500, PMCID: PMC7773353, DOI: 10.1172/jci140706.Peer-Reviewed Original ResearchConceptsT cellsSelf-lipid antigensSkin T cellsT cell autoreactivityT cell poolT cell repertoireMajor antigenic targetAntigenic targetsCell repertoireClinical diseaseCD1aSkin diseasesCD1 proteinsAntigenTCR recognitionCell poolEffector moleculesCD1a proteinLipid ligandsSmall antigensDiseaseHuman skinCellsAutoreactivityLipids
2020
Genome-wide association studies in Samoans give insight into the genetic architecture of fasting serum lipid levels
Carlson JC, Weeks DE, Hawley NL, Sun G, Cheng H, Naseri T, Reupena M, Tuitele J, Deka R, McGarvey ST, Minster RL. Genome-wide association studies in Samoans give insight into the genetic architecture of fasting serum lipid levels. Journal Of Human Genetics 2020, 66: 111-121. PMID: 32759990, PMCID: PMC7785639, DOI: 10.1038/s10038-020-0816-9.Peer-Reviewed Original ResearchMeSH KeywordsAdultApolipoprotein A-IApolipoproteins ECholesterol Ester Transfer ProteinsCholesterol, HDLCholesterol, LDLCohort StudiesFastingFemaleGenetic MarkersGenome-Wide Association StudyHumansLipid MetabolismLipidsMaleMiddle AgedPolymorphism, Single NucleotideQuantitative Trait LociSamoaTriglyceridesConceptsGenome-wide association studiesGenetic architectureAssociation studiesGenome-wide significant associationDifferent association signalsUnique population historyPopulation bottlenecksLipid lociPopulation historyAssociation signalsSuggestive associationRab21Current understandingMGAT1Association of variantsReplication cohortLipidsBiological foundationsVariantsLociLow-density lipoproteinInsightsPopulationLipid levelsHigh-density lipoproteinPhylogenetic and physiological signals in metazoan fossil biomolecules
Wiemann J, Crawford JM, Briggs DEG. Phylogenetic and physiological signals in metazoan fossil biomolecules. Science Advances 2020, 6: eaba6883. PMID: 32832604, PMCID: PMC7439315, DOI: 10.1126/sciadv.aba6883.Peer-Reviewed Original ResearchConceptsEvolutionary historyPhylogenetic informationMetazoan relationshipsMolecular signalsAnimal formsRelative abundanceDifferent tissue typesDeep timeLipoxidation productsTissue typesMetazoan fossilsMolecular compositionMolecular heterogeneityProteinBiological signalsFossil organic matterLipidsPowerful toolSugarsAbundanceLipoxidationGlycoxidationFossilsOrganic matterFossilization
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