2011
Combined Electron Transfer Dissociation–Collision-Induced Dissociation Fragmentation in the Mass Spectrometric Distinction of Leucine, Isoleucine, and Hydroxyproline Residues in Peptide Natural Products
Gupta K, Kumar M, Chandrashekara K, Krishnan KS, Balaram P. Combined Electron Transfer Dissociation–Collision-Induced Dissociation Fragmentation in the Mass Spectrometric Distinction of Leucine, Isoleucine, and Hydroxyproline Residues in Peptide Natural Products. Journal Of Proteome Research 2011, 11: 515-522. PMID: 22111579, DOI: 10.1021/pr200091v.Peer-Reviewed Original ResearchConceptsIsobaric residuesMass spectrometric peptide sequencingNatural peptide librariesPeptide natural productsCrude peptide mixturesTandem mass spectrometric methodMass spectrometric distinctionMass spectrometric methodRadical ionsIsobaric leucineElectron transferCollisional activationDissociation fragmentationPeptide mixturesNatural productsNominal massSpectrometric methodLiquid chromatographyPeptide sequencingPresence of hydroxyprolineIonsPeptide libraryDifferent peptidesDissociation approachHydroxyproline residues
2002
Automation of Nanoscale Microcapillary Liquid Chromatography−Tandem Mass Spectrometry with a Vented Column
Licklider L, Thoreen C, Peng J, Gygi S. Automation of Nanoscale Microcapillary Liquid Chromatography−Tandem Mass Spectrometry with a Vented Column. Analytical Chemistry 2002, 74: 3076-3083. PMID: 12141667, DOI: 10.1021/ac025529o.Peer-Reviewed Original ResearchConceptsPeptide mixturesSample introduction stepComplex peptide mixturesTandem MS experimentsMS analysisReversed phase LC-MS/MS analysisLiquid chromatography-tandem mass spectrometry analysisColumn enrichmentPeptide samplesElectrospray sourceMass spectrometry analysisMS experimentsCapillary columnLarge sample volumesMass spectrometryLC-MS/MS analysisColumn washingIntroduction stepChromatography fractionsLine separationSpectrometry analysisNano-LCLoop injectorSample volumeColumn
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