2025
Native Top-down analysis of membrane protein complexes directly from in vitro and native membranes
Jung W, Panda A, Lee J, Ghosh S, Shaw J, Gupta K. Native Top-down analysis of membrane protein complexes directly from in vitro and native membranes. Molecular & Cellular Proteomics 2025, 100993. PMID: 40378922, DOI: 10.1016/j.mcpro.2025.100993.Peer-Reviewed Original ResearchNative mass spectrometryProtein complexesMembrane proteinsBAM complexTop-down MS/MSAnalysis of membrane protein complexesMembrane-associated protein complexesTarget membrane proteinsNative membranesIntegral membrane proteinsTop-down identificationMembrane protein complexesCell-derived membrane vesiclesMass spectrometryMembrane vesiclesOrganization of proteinsDestabilize lipid bilayersAntibiotic targetsProtein-lipid complexesHeteromeric natureSynthetic liposomesCellular membranesCo-factorNative membrane vesiclesTotal membranes
2020
Dimeric Stilbene Antibiotics Target the Bacterial Cell Wall in Drug-Resistant Gram-Positive Pathogens
Goddard TN, Patel J, Park HB, Crawford JM. Dimeric Stilbene Antibiotics Target the Bacterial Cell Wall in Drug-Resistant Gram-Positive Pathogens. Biochemistry 2020, 59: 1966-1971. PMID: 32410442, PMCID: PMC10578317, DOI: 10.1021/acs.biochem.0c00213.Peer-Reviewed Original ResearchConceptsChemical complementation studiesBacterial cell wallMass spectrometry analysisLipid IILead compoundsMultidrug-resistant Gram-positive pathogensStilbene dimersCell wall precursorsSpectrometry analysisDrug-Resistant GramNew antimicrobial agentsGram-positive pathogensDimersNonlethal exposureAntibiotic targetsPositive pathogensDietary plantsAction studiesAntibiotic resistanceMode of actionAntimicrobial agentsLead dimerWall precursorsMonomersStrong activity
2015
An Activity‐Based Probe for Studying Crosslinking in Live Bacteria
Gautam S, Kim T, Shoda T, Sen S, Deep D, Luthra R, Ferreira M, Pinho M, Spiegel D. An Activity‐Based Probe for Studying Crosslinking in Live Bacteria. Angewandte Chemie 2015, 127: 10638-10642. DOI: 10.1002/ange.201503869.Peer-Reviewed Original ResearchWall teichoic acidActivity-based probesSingle PBPLive bacteriaAntibiotic targetsCell wallFluorescent mimicTeichoic acidNatural substrateLive cellsEssential processRelative quantitationNatural crosslinksPeptidoglycanBacterial growthSpatiotemporal aspectsBacteriaExperimental toolPBPPeptide mimicsMimics
2008
Riboswitches as new antibiotics targets
Blount K, Breaker R. Riboswitches as new antibiotics targets. The FASEB Journal 2008, 22: 264.3-264.3. DOI: 10.1096/fasebj.22.1_supplement.264.3.Peer-Reviewed Original ResearchDrug targetsAntibacterial drug discoveryNew antibiotic targetsNew cellular targetsDrug discoveryAntibiotic targetsRiboswitchGene expressionRNA structureCellular targetsDrug discoverersAntibacterial compoundsGenesLethal effectsBacteriaExpressionMetabolite analogsReceptorsTargetDiscoveryRNABindsSelective receptorsMRNARecent work
This site is protected by hCaptcha and its Privacy Policy and Terms of Service apply