Public Health Interests
Environmental Health; Evolution
Extensive Research Description
My lab is interested in somatosensation and thermoregulation, particularly molecular and evolutionary mechanisms whereby the somatosensory and thermoregulatory systems adapt to the environmental and behavioral needs of an organism. We intend:
(i) To understand, which molecules mediate different types of sensation undernormal and extreme physiological conditions using mammalian hibernation as a naturally reversible model.
(ii) To dissect mechanism(s) of thermoregulation and thermogenesis using hibernators in their active and torpor physiological states.
Mammalian hibernation is fascinating as it is characterized by prolonged alternating periods of hypothermia (core body temperature drops from 37°C to 2-10°C) in association with unusual resistance of tissues to cold. Despite the robustness of these phenomena, fundamental questions remain about their cellular basis. Mammalian hibernators (thirteen-lined ground squirrels and Syrian hamsters) provide unique natural system for understanding thermotransduction machinery. Moreover, comparisons between phylogenetically related species of hibernators and non-hibernators will provide insights into anatomical, physiological, and genetic factors that support this unique thermo-adaptive process. Due to the complexity and dynamic nature of thesomatosensory and thermoregulatory systems, we are taking an integrated approach using biochemistry, bioinformatics, live-cell imaging, electrophysiology, genomics, behavioral paradigms, and additional cellular and molecular biological techniques to approach these fascinating questions of both physiological and clinical significance.
Mechanisms gleaned from this study could have profound outcomes for human health in regard to:
(i) Inducible and reversible hypothermia.
(ii) Cold tolerance and hypersensitivity.
(iii) Tissue transplantation.
Molecular Prerequisites for Diminished Cold Sensitivity in Ground Squirrels and Hamsters.
Matos-Cruz V, Schneider ER, Mastrotto M, Merriman DK, Bagriantsev SN, Gracheva EO. Molecular Prerequisites for Diminished Cold Sensitivity in Ground Squirrels and Hamsters. Cell Reports 2017, 21:3329-3337. 2017
Low-cost functional plasticity of TRPV1 supports heat tolerance in squirrels and camels
Laursen WJ, Schneider ER, Merriman DK, Bagriantsev SN, Gracheva EO PNAS, 2016 Oct 4;113(40):11342-11347. Epub 2016 Sep 16. 2016
Neuronal UCP1 expression suggests a mechanism for local thermogenesis during hibernation.
Laursen WJ, Mastrotto M, Pesta D, Funk OH, Goodman JB, Merriman DK, Ingolia N, Shulman GI, Bagriantsev SN, Gracheva EO. Neuronal UCP1 expression suggests a mechanism for local thermogenesis during hibernation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2015, 112:1607-12. 2015
Ganglion-specific splicing of TRPV1 underlies infrared sensation in vampire bats.
Gracheva EO, Cordero-Morales JF, González-Carcacía JA, Ingolia NT, Manno C, Aranguren CI, Weissman JS, Julius D. Ganglion-specific splicing of TRPV1 underlies infrared sensation in vampire bats. Nature 2011, 476:88-91. 2011
Molecular basis of infrared detection by snakes.
Gracheva EO, Ingolia NT, Kelly YM, Cordero-Morales JF, Hollopeter G, Chesler AT, Sánchez EE, Perez JC, Weissman JS, Julius D. Molecular basis of infrared detection by snakes. Nature 2010, 464:1006-11. 2010