2024
Hypothalamic hormone deficiency enables physiological anorexia in ground squirrels during hibernation
Mohr S, Dai Pra R, Platt M, Feketa V, Shanabrough M, Varela L, Kristant A, Cao H, Merriman D, Horvath T, Bagriantsev S, Gracheva E. Hypothalamic hormone deficiency enables physiological anorexia in ground squirrels during hibernation. Nature Communications 2024, 15: 5803. PMID: 38987241, PMCID: PMC11236985, DOI: 10.1038/s41467-024-49996-2.Peer-Reviewed Original ResearchConceptsHypothalamic feeding centersHormone deficiencyHypothalamic arcuate nucleus neuronsArcuate nucleus neuronsThyroid hormone deficiencyFeeding centerEffects of ghrelinAnorexigenic effectNucleus neuronsPhysiological anorexiaThyroid hormonesNormal physiological functionsGround squirrelsInterbout arousalAnorexiaThirteen-lined ground squirrelsProlonged periodReduced sensitivityPhysiological functionsDeficiencyFrequency-modulated timer regulates torpor–arousal cycles during hibernation in distinct small mammalian hibernators
Gibo S, Yamaguchi Y, Gracheva E, Bagriantsev S, Tokuda I, Kurosawa G. Frequency-modulated timer regulates torpor–arousal cycles during hibernation in distinct small mammalian hibernators. Npj Biological Timing And Sleep 2024, 1: 3. DOI: 10.1038/s44323-024-00002-4.Peer-Reviewed Original ResearchTorpor-arousal cycleMolecular mechanisms of hibernationMammalian hibernationGround squirrelsHibernation patternsTb fluctuationsBiological processesHarsh seasonsHibernating speciesDeep torporMolecular mechanismsConstant laboratory conditionsSyrian hamstersHibernationTB dataEndogenous periodEuthermic levelsTorporPhysiological conditionsMechanisms of hibernationBasal metabolismAmbient levelsCircannual rhythmPeriods of arousalSquirrelsNeural control of fluid homeostasis is engaged below 10°C in hibernation
Junkins M, Feng N, Murphy L, Curtis G, Merriman D, Bagriantsev S, Gracheva E. Neural control of fluid homeostasis is engaged below 10°C in hibernation. Current Biology 2024, 34: 923-930.e5. PMID: 38325375, PMCID: PMC11232715, DOI: 10.1016/j.cub.2024.01.035.Peer-Reviewed Original ResearchConceptsAntidiuretic hormone arginine vasopressinSupraoptic nucleusFluid homeostasisC-Fos immunohistochemistryControl of fluid homeostasisHeart rateHypothalamic supraoptic nucleusReduced plasma levelsHormone arginine vasopressinPlasma hormone levelsSlow heart rateSON neuronsArginine vasopressinMassive fluid lossPlasma levelsPosterior pituitaryHormone levelsFiber photometryC-fosBlood pressureNeuronal activityHormonal responsesThirteen-lined ground squirrelsOxytocinInterbout arousal
2023
3D architecture and a bicellular mechanism of touch detection in mechanosensory corpuscle
Nikolaev Y, Ziolkowski L, Pang S, Li W, Feketa V, Xu C, Gracheva E, Bagriantsev S. 3D architecture and a bicellular mechanism of touch detection in mechanosensory corpuscle. Science Advances 2023, 9: eadi4147. PMID: 37703368, PMCID: PMC10499330, DOI: 10.1126/sciadv.adi4147.Peer-Reviewed Original ResearchGround squirrels – experts in thermoregulatory adaptation
Feketa V, Bagriantsev S, Gracheva E. Ground squirrels – experts in thermoregulatory adaptation. Trends In Neurosciences 2023, 46: 505-507. PMID: 37188617, DOI: 10.1016/j.tins.2023.04.008.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsHuman TRPV1 structure and inhibition by the analgesic SB-366791
Neuberger A, Oda M, Nikolaev Y, Nadezhdin K, Gracheva E, Bagriantsev S, Sobolevsky A. Human TRPV1 structure and inhibition by the analgesic SB-366791. Nature Communications 2023, 14: 2451. PMID: 37117175, PMCID: PMC10147690, DOI: 10.1038/s41467-023-38162-9.Peer-Reviewed Original ResearchConceptsSB-366791Transient receptor potential (TRP) ion channelsPotential ion channelsPain pathwaysPain therapyPain treatmentPsychiatric disordersOpioid crisisTherapy targetTRPV1 inhibitorElectrophysiological recordingsHuman TRPV1TRP channelsTRPV1New drugsDisease conditionsVanilloid subfamilyIon channelsTreatmentInhibitorsOpioidsPainTherapyDiseaseCryo-electron microscopy structureMechanotransduction events at the physiological site of touch detection
Ziolkowski L, Gracheva E, Bagriantsev S. Mechanotransduction events at the physiological site of touch detection. ELife 2023, 12: e84179. PMID: 36607222, PMCID: PMC9833821, DOI: 10.7554/elife.84179.Peer-Reviewed Original Research
2020
CNGA3 acts as a cold sensor in hypothalamic neurons
Feketa VV, Nikolaev YA, Merriman DK, Bagriantsev SN, Gracheva EO. CNGA3 acts as a cold sensor in hypothalamic neurons. ELife 2020, 9: e55370. PMID: 32270761, PMCID: PMC7182431, DOI: 10.7554/elife.55370.Peer-Reviewed Original ResearchPiezo2 Integrates Mechanical and Thermal Cues in Vertebrate Mechanoreceptors
Nikolaev Y, Zheng W, Gracheva E, Bagriantsev S. Piezo2 Integrates Mechanical and Thermal Cues in Vertebrate Mechanoreceptors. Biophysical Journal 2020, 118: 396a. DOI: 10.1016/j.bpj.2019.11.2254.Peer-Reviewed Original Research
2019
Osmolyte Depletion and Thirst Suppression Allow Hibernators to Survive for Months without Water
Feng NY, Junkins MS, Merriman DK, Bagriantsev SN, Gracheva EO. Osmolyte Depletion and Thirst Suppression Allow Hibernators to Survive for Months without Water. Current Biology 2019, 29: 3053-3058.e3. PMID: 31495581, PMCID: PMC6759396, DOI: 10.1016/j.cub.2019.07.038.Peer-Reviewed Original Research
2018
Somatosensory Neurons Enter a State of Altered Excitability during Hibernation
Hoffstaetter LJ, Mastrotto M, Merriman DK, Dib-Hajj SD, Waxman SG, Bagriantsev SN, Gracheva EO. Somatosensory Neurons Enter a State of Altered Excitability during Hibernation. Current Biology 2018, 28: 2998-3004.e3. PMID: 30174191, PMCID: PMC6173314, DOI: 10.1016/j.cub.2018.07.020.Peer-Reviewed Original ResearchConceptsSomatosensory neuronsAction potentialsPeripheral somatosensory neuronsVoltage-gated sodium channelsTherapeutic hypothermiaAltered excitabilityFunctional deficitsCase of neuronsSensory functionFiring patternsNeuronsCold exposureInput resistanceSodium channelsRapid restorationBody temperatureHypometabolismHypothermiaDevelopment of strategiesSensory informationProlonged periodAdaptive responseArousalCNSExcitability
2017
Molecular Prerequisites for Diminished Cold Sensitivity in Ground Squirrels and Hamsters
Matos-Cruz V, Schneider ER, Mastrotto M, Merriman DK, Bagriantsev SN, Gracheva EO. Molecular Prerequisites for Diminished Cold Sensitivity in Ground Squirrels and Hamsters. Cell Reports 2017, 21: 3329-3337. PMID: 29262313, PMCID: PMC5741102, DOI: 10.1016/j.celrep.2017.11.083.Peer-Reviewed Original ResearchConceptsTransmembrane domainCold toleranceCold sensitivityGround squirrelsRat orthologMolecular adaptationsMammalian hibernatorsReciprocal mutationsAmino acidsFunctional significanceOrthologsSquirrelsMolecular prerequisitesActive stateHibernatorsTRPM8Somatosensory neuronsTolerancePoor activationSpeciesDomainMutationsResiduesHibernationCells
2016
Low-cost functional plasticity of TRPV1 supports heat tolerance in squirrels and camels
Laursen WJ, Schneider ER, Merriman DK, Bagriantsev SN, Gracheva EO. Low-cost functional plasticity of TRPV1 supports heat tolerance in squirrels and camels. Proceedings Of The National Academy Of Sciences Of The United States Of America 2016, 113: 11342-11347. PMID: 27638213, PMCID: PMC5056056, DOI: 10.1073/pnas.1604269113.Peer-Reviewed Original ResearchMeSH KeywordsAfferent PathwaysAmino Acid SequenceAmino Acid SubstitutionAnimalsAnkyrin RepeatCamelusCapsaicinConserved SequenceGanglia, SpinalHEK293 CellsHot TemperatureHumansHydrogen-Ion ConcentrationIon Channel GatingMutationNeuronsSciuridaeSequence AlignmentThermotoleranceTRPV Cation ChannelsXenopusConceptsHeat toleranceCommon molecular strategyN-terminal ankyrinSingle amino acid changeSingle amino acid substitutionGround squirrelsPolymodal ion channelAmino acid changesAmino acid substitutionsRemarkable functional flexibilityFunctional conservationEcological nichesChemical cuesMammalian speciesMolecular strategiesAcid changesAcid substitutionsHeat sensitivityTransient receptor potential vanilloid 1Ion channelsOrthologsFunctional plasticitySquirrelsBactrian camelsSuch adaptationStretch-Gated Ion Channels in Neuronal Mechanoreceptors
Bagriantsev S, Schneider E, Anderson E, Matson J, Gracheva E. Stretch-Gated Ion Channels in Neuronal Mechanoreceptors. Biophysical Journal 2016, 110: 349a. DOI: 10.1016/j.bpj.2015.11.1876.Peer-Reviewed Original ResearchProbing the Contribution of Nav1.7 and Nav1.8 to Cold Tolerance in Hibernators
Hoffstaetter L, Tonsfeldt K, Matos-Cruz V, Bagriantsev S, Gracheva E. Probing the Contribution of Nav1.7 and Nav1.8 to Cold Tolerance in Hibernators. Biophysical Journal 2016, 110: 318a. DOI: 10.1016/j.bpj.2015.11.1710.Peer-Reviewed Original ResearchInvestigating the Role of Nav1.5 in Somatosensory Mechanosensation
Anderson E, Schneider E, Matson J, Gracheva E, Bagriantsev S. Investigating the Role of Nav1.5 in Somatosensory Mechanosensation. Biophysical Journal 2016, 110: 91a. DOI: 10.1016/j.bpj.2015.11.552.Peer-Reviewed Original Research
2015
Neuronal UCP1 expression suggests a mechanism for local thermogenesis during hibernation
Laursen WJ, Mastrotto M, Pesta D, Funk OH, Goodman JB, Merriman DK, Ingolia N, Shulman GI, Bagriantsev SN, Gracheva EO. Neuronal UCP1 expression suggests a mechanism for local thermogenesis during hibernation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2015, 112: 1607-1612. PMID: 25605929, PMCID: PMC4321293, DOI: 10.1073/pnas.1421419112.Peer-Reviewed Original ResearchConceptsSummer active stateNervous tissue functionPalmitate-induced uncouplingDifferential transcriptomicsMolecular mechanismsHibernating mammalsBrown adipose tissueBody temperatureProtein 1Ground squirrelsBiochemical analysisTorpid squirrelsHibernation seasonTissue functionActive stateUCP1 expressionHigh expressionHibernationPharmacologic analysisThermogenic proteinsAdipose tissueBrain temperatureLow body temperatureNervous systemPhysiologic phenotypeSensing Force by Trigeminal Neurons of Acutely Mechanosensitive Birds
Schneider E, Mastrotto M, Laursen W, Schulz V, Goodman J, Funk O, Gallagher P, Gracheva E, Bagriantsev S. Sensing Force by Trigeminal Neurons of Acutely Mechanosensitive Birds. Biophysical Journal 2015, 108: 562a. DOI: 10.1016/j.bpj.2014.11.3080.Peer-Reviewed Original Research
2014
Molecular Adaptations to Extreme Thermogenesis in Mammalian Hibernators
Laursen W, Funk O, Goodman J, Merriman D, Ingolia N, Bagriantsev S, Gracheva E. Molecular Adaptations to Extreme Thermogenesis in Mammalian Hibernators. Biophysical Journal 2014, 106: 337a. DOI: 10.1016/j.bpj.2013.11.1931.Peer-Reviewed Original Research
2012
Cytoplasmic Ankyrin Repeats of Transient Receptor Potential A1 (TRPA1) Dictate Sensitivity to Thermal and Chemical Stimuli
Cordero-Morales J, Gracheva E, Julius D. Cytoplasmic Ankyrin Repeats of Transient Receptor Potential A1 (TRPA1) Dictate Sensitivity to Thermal and Chemical Stimuli. Biophysical Journal 2012, 102: 23a. DOI: 10.1016/j.bpj.2011.11.151.Peer-Reviewed Original Research