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INFORMATION FOR

Frozen Sections (Cryotomy)

Optimal for some immunohistochemical staining protocols, preservation of fluorescent cell markers (GFP, tomato) and lipids in tissues. Please contact the lab for preferred fixation methods for this service (varies depending on tissues and staining requirements).

Preparation Instructions

Fixation/freezing protocols vary depending on stain/immunostain. Please contact the lab for specific instructions prior to starting your project.


Please Note

We strongly recommend that you contact the lab before you start your experiment to consult on the most appropriate preparation to maximize success of your work.

  • Photo

    Christiane Coady operates a cryostat to cut frozen sections of tissue. Tissues prepared in this manner are often used for immunohistochemistry (IHC), fluorescence (GFP), in situ hybridization (FISH) or lipid staining (Oil Red O).
    Photo by Robert A. Lisak

  • Photo

    Oil red O stain indicating areas of lipids in a frozen bone section.