2002
Mitochondrial deletions in luteinized granulosa cells as a function of age in women undergoing in vitro fertilization
Seifer DB, DeJesus V, Hubbard K. Mitochondrial deletions in luteinized granulosa cells as a function of age in women undergoing in vitro fertilization. Fertility And Sterility 2002, 78: 1046-1048. PMID: 12413991, DOI: 10.1016/s0015-0282(02)04214-0.Peer-Reviewed Original ResearchConceptsGranulosa cellsAdvanced reproductive ageLuteinized granulosa cellsMitochondrial DNA deletionsGonadotropin stimulationReproductive ageMAIN OUTCOMEFollicular aspirationFertility clinicsFunction of ageYoung womenWomenIVFAgeDNA deletionsCellsMitochondrial deletionsYearsNormal mitochondriaNormal mtDNAPatientsDeletionClinicSubstantial decreaseDNA microarray analysis of the expression profiles of luteinized granulosa cells as a function of ovarian reserve
Chin KV, Seifer DB, Feng B, Lin Y, Shih WC. DNA microarray analysis of the expression profiles of luteinized granulosa cells as a function of ovarian reserve. Fertility And Sterility 2002, 77: 1214-1218. PMID: 12057731, DOI: 10.1016/s0015-0282(02)03114-x.Peer-Reviewed Original ResearchConceptsLuteinized granulosa cellsOvarian reserveDNA microarray analysisGranulosa cellsExpression profilesMicroarray analysisSequence tagsFertilization-embryo transferUniversity-based fertility clinicExpression profilingComplementary DNA microarray analysisGonadotropin stimulationFollicular aspirationMAIN OUTCOMEFertility clinicsWomenDNA microarraysGene expressionCellsGenesProfilingPatientsIVFClinicSignature patterns
1996
Apoptosis as a function of ovarian reserve in women undergoing in vitro fertilization*†
Seifer D, Gardiner A, Ferreira K, Peluso J. Apoptosis as a function of ovarian reserve in women undergoing in vitro fertilization*†. Fertility And Sterility 1996, 66: 593-598. PMID: 8816622, DOI: 10.1016/s0015-0282(16)58573-2.Peer-Reviewed Original ResearchConceptsDay 3 serum FSHFSH levelsOvarian reserveSerum FSHMIU/Granulosa cellsCell countDiminished ovarian reserveMIU/mLAcademic clinical practiceLuteinized granulosa cellsApoptotic granulosa cellsIVF-ETFollicular aspiratesPreovulatory folliclesClinical practiceFlow cytometryWomenMean numberApoptotic cellsFSHGreater percentageFolliclesCellsViable cellsAutologous granulosa cell coculture demonstrates zygote suppression of granulosa cell steroidogenesis**Presented in part at the 51st Annual Meeting of the American Society for Reproductive Medicine. Seattle, Washington, October 7 to 12, 1995.††Supported in part by Physician Scientist Award (AG00566 [D.B.S.] and R01HD31894 [A.L.S.]) from the National Institutes of Health, National Institute on Aging, Bethesda, Maryland.
Seifer D, Freeman M, Gardiner A, Hill G, Schneyer A, Vanderhyden B. Autologous granulosa cell coculture demonstrates zygote suppression of granulosa cell steroidogenesis**Presented in part at the 51st Annual Meeting of the American Society for Reproductive Medicine. Seattle, Washington, October 7 to 12, 1995.††Supported in part by Physician Scientist Award (AG00566 [D.B.S.] and R01HD31894 [A.L.S.]) from the National Institutes of Health, National Institute on Aging, Bethesda, Maryland. Fertility And Sterility 1996, 66: 425-429. PMID: 8751742, DOI: 10.1016/s0015-0282(16)58513-6.Peer-Reviewed Original ResearchConceptsLuteinized granulosa cellsGranulosa cellsStandard IVF-ET treatment cycleIVF-ET treatment cyclesTwo-pronuclear embryosGranulosa cell steroidogenesisTwo-pronuclear zygotesNational InstituteVitro Fertilization UnitProduction of E2Steroid hormone productionPituitary desensitizationOvarian stimulationFollicular aspiratesCell steroidogenesisFertilization unitTreatment cyclesHormone productionCell coculturesReproductive medicineP productionAmerican SocietyAcademic research environmentE2Human embryosDifferential secretion of dimeric inhibin in cultured luteinized granulosa cells as a function of ovarian reserve
Seifer D. Differential secretion of dimeric inhibin in cultured luteinized granulosa cells as a function of ovarian reserve. The Journal Of Clinical Endocrinology & Metabolism 1996, 81: 736-739. DOI: 10.1210/jc.81.2.736.Peer-Reviewed Original ResearchDifferential secretion of dimeric inhibin in cultured luteinized granulosa cells as a function of ovarian reserve
Seifer DB, Gardiner AC, Lambert-Messerlian G, Schneyer AL. Differential secretion of dimeric inhibin in cultured luteinized granulosa cells as a function of ovarian reserve. The Journal Of Clinical Endocrinology & Metabolism 1996, 81: 736-739. PMID: 8636296, DOI: 10.1210/jcem.81.2.8636296.Peer-Reviewed Original ResearchConceptsDay 3 serum FSHLuteinized granulosa cellsHigh FSH groupDimeric inhibinSerum FSHFSH groupPg/Granulosa cellsFSH levelsOvarian reserveOvulation inductionProgesterone concentrationsDay 3 serum FSH levelsCultured luteinized granulosa cellsDimeric inhibin productionLow FSH groupSerum FSH levelsFertilization-embryo transferHigher FSH levelsReproductive technology programGranulosa cell levelTotal inhibinInhibin concentrationsInhibin productionProspective study
1993
Proliferative index of human luteinized granulosa cells varies as a function of ovarian reserve
Seifer D, Charland C, Berlinsky D, Penzias A, Haning R, Naftolin F, Barker B. Proliferative index of human luteinized granulosa cells varies as a function of ovarian reserve. American Journal Of Obstetrics And Gynecology 1993, 169: 1531-1535. PMID: 8267057, DOI: 10.1016/0002-9378(93)90430-q.Peer-Reviewed Original ResearchConceptsSerum follicle-stimulating hormone levelsFollicle-stimulating hormone levelsFollicle-stimulating hormoneLuteinized granulosa cellsIU/L.Ovarian reserveHormone levelsProliferative indexGranulosa cellsHormone groupChronologic agePreovulatory folliclesDay 3 follicle-stimulating hormone levelsHigh follicle-stimulating hormoneLower follicle-stimulating hormoneSerum follicle-stimulating hormoneFlow cytometryHuman luteinized granulosa cellsGreater proliferative indexSame chronologic ageWoman's ovarian reserveOvulation induction protocolsProspective cohort studyAdvanced reproductive ageIU/LGonadotropin-releasing hormone agonist-induced differences in granulosa cell cycle kinetics are associated with alterations in follicular fluid müllerian-inhibiting substance and androgen content
Seifer DB, MacLaughlin DT, Penzias AS, Behrman HR, Asmundson L, Donahoe PK, Haning RV, Flynn SD. Gonadotropin-releasing hormone agonist-induced differences in granulosa cell cycle kinetics are associated with alterations in follicular fluid müllerian-inhibiting substance and androgen content. The Journal Of Clinical Endocrinology & Metabolism 1993, 76: 711-714. PMID: 8445031, DOI: 10.1210/jcem.76.3.8445031.Peer-Reviewed Original ResearchConceptsHuman menopausal gonadotropinCell cycle kineticsProliferative indexGranulosa cellsFollicular fluidControl groupCycle kineticsGnRH analogue leuprolideOvulation induction regimensFollicular fluid contentMüllerian-inhibiting substanceHuman granulosa cellsLuteinized granulosa cellsCI (3.2-3.6) PInduction regimensMenopausal gonadotropinOocyte retrievalAndrogen contentGnRHSpecific growth factorsFlow cytometryGrowth factorFolliclesFluid contentOne-way analysis