Other Post Translational Modifications
Other post translational modifications besides phosphorylation that are commonly searched for and identified include but are not limited to ubiquitination1, SUMOylation, methylation, acetylation, and decarboxylation. Procedures are also in place for identification of sites of palmitoylation2 and glycosylation. Samples can be submitted in gel bands or in solution.
The solution samples will be brought through a clean-up step using an extraction or precipitation method prior to digestion in order to remove buffer components.
Suitable Protein Complexity
|PTM-other-gel||In gel digestion looking for other PTMS such as ubiquitination||In gel digestion using a single enzyme, LC-MS/MS on a short length gradient, and a database search looking for requested PTM|
|PTM-other-solution-<50 proteins||Solution digestion with less than 50 proteins in the sample and looking for other types of PTMS||Sample clean-up, a single enzyme digestion, LC-MS/MS on a short length gradient, and a database search looking for requested PTM|
|PTM-other-solution-<500 proteins||solution digestion with a more complex proteome||Sample clean-up, a single enzyme digestion, LC-MS/MS on a long length gradient, and a database search looking for requested PTM|
- Shibata, S., Zhang, J., Puthumana, J., Stone, K., Lifton, R. (2013) Kelch-like 3 and Cullin 3 regulate electrolyte homeostasis via ubiquitination and degradation of WNK4. PNAS,110, 7838-7843
(PMCID: PMC3651502) (PMID:23576762).
- Antinone, S.E., Ghadge, G.D., Lam, T.T., Wang, L., Roos, R., and Green, W.N. (2013) Palmitoylation of superoxide dismutase 1 (SOD1) is increased for familial amyotrophic lateral sclerosis-linked SOD1 mutants. J. Biol. Chem. 288:21606-21617 (PMCID: PMC3724620) (PMID: 23760509).
- Sample Submission – YPED