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Methylation, Acetylation, Dimethylation

These types of modifications are typically located by comparative peptide mapping on either the FT-ICR, MALDI-Tof/Tof instruments, or by LC-MS/MS analysis.

Approaches are

  • Comparative mapping of the peptide mass fingerprint of a control sample digestion (with no modification) to the modified sample digestion using µESI FT-ICR or MALDI-Tof/Tof MS. Masses that differ can be further analyzed by MS/MS.

Important Links

Figure 1 shows the use of accurate mass from direct infusion µESI FT-ICR MS to locate a site of methylation from a purified protein.
  • Comparison of the observed peptide mass fingerprint masses (µESI FT-ICR or MALDI-Tof/Tof) to the known protein sequence to locate peptides that do not match. MS/MS can be performed on these masses.
  • Analyze the digest(s) by LC-MS/MS and do a database search locating for specific modifications.