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INFORMATION FOR

Large Scale phosphoprotein Profiling

Uses both SCX fractionation and TiO2 affinity based phosphopeptide enrichment methodologies to identify large number of phosphopeptides from complex protein extracts.

Requirements: For identification of optimum number of phosphopeptides, 1 mg or more starting protein amounts are preferred. However, as low as 200 μg of starting protein material can be accepted.

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