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2D gels and Pro-Q-diamond staining

Using a Differential 2-D gel electrophoresis (DIGE™) experiment, phosphoproteins can be located using the Invitrogen Pro-Q Diamond fluorescent stain. The workflow is outlined in the figure.


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With this approach, phosphoproteins can be quantitated using the Cy3 channel for Pro-Q
Diamond phosphoprotein location, and Cy2 and Cy5 channels for determining protein
expression differences.