2022
Regulatory mechanisms of lipopolysaccharide synthesis in Escherichia coli
Shu S, Mi W. Regulatory mechanisms of lipopolysaccharide synthesis in Escherichia coli. Nature Communications 2022, 13: 4576. PMID: 35931690, PMCID: PMC9356133, DOI: 10.1038/s41467-022-32277-1.Peer-Reviewed Original ResearchMeSH KeywordsAmidohydrolasesEscherichia coliEscherichia coli ProteinsGram-Negative BacteriaLipopolysaccharidesMembrane ProteinsConceptsRegulatory mechanismsAnti-adaptor proteinsFirst committed stepMost Gram-negative bacteriaEssential glycolipidEssential membraneGram-negative bacteriaTransmembrane helicesAdaptor proteinCommitted stepCytoplasmic domainFtsHLPS synthesisAnalysis unravelsLipopolysaccharide synthesisLapBEscherichia coliE. coliPermeability barrierProtein levelsLpxCProtease activityProteinColiYejM
2021
A human apolipoprotein L with detergent-like activity kills intracellular pathogens
Gaudet RG, Zhu S, Halder A, Kim BH, Bradfield CJ, Huang S, Xu D, Mamiñska A, Nguyen TN, Lazarou M, Karatekin E, Gupta K, MacMicking JD. A human apolipoprotein L with detergent-like activity kills intracellular pathogens. Science 2021, 373 PMID: 34437126, PMCID: PMC8422858, DOI: 10.1126/science.abf8113.Peer-Reviewed Original ResearchMeSH KeywordsApolipoproteins LBacterial Outer MembraneBacteriolysisCell MembraneCell Membrane PermeabilityCells, CulturedCRISPR-Cas SystemsCytosolDetergentsGene EditingGram-Negative BacteriaGTP-Binding ProteinsHumansImmunity, InnateInterferon-gammaLipoproteinsMicrobial ViabilityO AntigensProtein DomainsSalmonella typhimuriumSolubilityConceptsSingle-particle cryo-electron microscopyCell-autonomous defenseCytosol-invasive bacteriaExpression of hundredsNative mass spectrometryCryo-electron microscopyHuman genesDetergent-like activityHost proteinsLipoprotein nanodiscsMammalian lipidsExtracellular transportImmune cytokine interferonCell typesDetergent-like propertiesApolipoprotein LLife-threatening infectionsPotent bactericidal agentsAnionic membranesProteinCytokine interferonNonimmune cellsMass spectrometryCellsMutagenesisAntibacterial Resistance Leadership Group 2.0: Back to Business
Chambers HF, Evans SR, Patel R, Cross HR, Harris AD, Doi Y, Boucher HW, van Duin D, Tsalik EL, Holland TL, Pettigrew MM, Tamma PD, Hodges KR, Souli M, Fowler VG. Antibacterial Resistance Leadership Group 2.0: Back to Business. Clinical Infectious Diseases 2021, 73: 730-739. PMID: 33588438, PMCID: PMC8366825, DOI: 10.1093/cid/ciab141.Peer-Reviewed Original ResearchMeSH KeywordsAnti-Bacterial AgentsDrug Resistance, BacterialGram-Negative BacteriaGram-Positive BacteriaLeadership
2020
The blind men and the filament: Understanding structures and functions of microbial nanowires
Yalcin SE, Malvankar NS. The blind men and the filament: Understanding structures and functions of microbial nanowires. Current Opinion In Chemical Biology 2020, 59: 193-201. PMID: 33070100, PMCID: PMC7736336, DOI: 10.1016/j.cbpa.2020.08.004.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCytochromesElectron TransportFimbriae, BacterialGeobacterGram-Negative BacteriaModels, MolecularNanowiresConceptsMicrobial nanowiresExtracellular electron transferDifferent nanowiresNanowiresPotential applicationsCryo-electron microscopyTransport electronsMultimodal functional imagingElectron transferBioelectronicsImportant environmental processesRecent discoveryPhysiological studiesOMCApplicationsMicroscopyBiofuelsSensingPhysiological needsMicrometersCrucial roleUnderstanding structureDirect evidenceThousands of papersEnergy productionGuanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms
Wandel MP, Kim BH, Park ES, Boyle KB, Nayak K, Lagrange B, Herod A, Henry T, Zilbauer M, Rohde J, MacMicking JD, Randow F. Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms. Nature Immunology 2020, 21: 880-891. PMID: 32541830, PMCID: PMC7381384, DOI: 10.1038/s41590-020-0697-2.Peer-Reviewed Original ResearchConceptsGuanylate-binding proteinsCaspase-4 activationCaspase-4Human caspase-4Pyroptotic cell deathGram-negative bacteriaCytosolic bacteriaReplicative nicheEvolutionary evidenceIntracellular bacteriaCell deathMultiple antagonistsNeighboring cellsCaspase-11BacteriaAntibacterial defenseBacterial challengeGasderminShigella flexneriProteinDependent pyroptosisActivationPathwayBacterial lipopolysaccharideGBP2
2019
IFN-mediated negative feedback supports bacteria class-specific macrophage inflammatory responses
Gottschalk R, Dorrington M, Dutta B, Krauss K, Martins A, Uderhardt S, Chan W, Tsang J, Torabi-Parizi P, Fraser I, Germain R. IFN-mediated negative feedback supports bacteria class-specific macrophage inflammatory responses. ELife 2019, 8: e46836. PMID: 31385572, PMCID: PMC6684266, DOI: 10.7554/elife.46836.Peer-Reviewed Original ResearchConceptsContext-dependent regulationGram-positive speciesGram-negative bacteriaClass-specific mannerInflammatory responseRegulatory eventsMolecular mechanismsMacrophage inflammatory responseMouse macrophagesLigand pairsInnate immunityInflammatory cytokine productionMacrophage responseBacteriaRegulationSpecific pathogensIL-10Cytokine productionLung infectionProduction dynamicsInhibitory eventsSpeciesMacrophagesNegative feedbackInflammation dynamicsBloodstream infections caused by multidrug-resistant gram-negative bacteria: epidemiological, clinical and microbiological features
Leal H, Azevedo J, Silva G, Amorim A, de Roma L, Arraes A, Gouveia E, Reis M, Mendes A, de Oliveira Silva M, Barberino M, Martins I, Reis J. Bloodstream infections caused by multidrug-resistant gram-negative bacteria: epidemiological, clinical and microbiological features. BMC Infectious Diseases 2019, 19: 609. PMID: 31296179, PMCID: PMC6624930, DOI: 10.1186/s12879-019-4265-z.Peer-Reviewed Original ResearchConceptsK. pneumoniaePrevious antimicrobial useLaboratory-based surveillanceAntimicrobial susceptibility testingMDR bacteremiaMDR-GNBDrug-resistant pathogensNegative bacteremiaLiver diseaseBloodstream infectionsHigh morbidityMale sexRisk factorsEpidemiological aspectsNegative bacilliHealthcare professionalsMicrobiological featuresSusceptibility testingBacteremiaInfectionAntimicrobial useCausative agentAntibiotic resistanceIntervention measuresResistance determinantsEvidence for phospholipid export from the bacterial inner membrane by the Mla ABC transport system
Hughes G, Hall S, Laxton C, Sridhar P, Mahadi A, Hatton C, Piggot T, Wotherspoon P, Leney A, Ward D, Jamshad M, Spana V, Cadby I, Harding C, Isom G, Bryant J, Parr R, Yakub Y, Jeeves M, Huber D, Henderson I, Clifton L, Lovering A, Knowles T. Evidence for phospholipid export from the bacterial inner membrane by the Mla ABC transport system. Nature Microbiology 2019, 4: 1692-1705. PMID: 31235958, DOI: 10.1038/s41564-019-0481-y.Peer-Reviewed Original ResearchConceptsMla pathwayInner membraneAsymmetric Gram-negative outer membranePhospholipid transportRetrograde phospholipid transportGram-negative outer membranePhospholipid-binding pocketABC transport systemSoluble periplasmic proteinBacterial inner membraneInner membrane ATPaseIndependent of ATPClosed apo conformationPhospholipid exportPeriplasmic proteinsMlaFEDB complexOuter membraneApo formApo conformationLipid exportMembrane ATPaseExport processPathwayMembraneMLACThe Injectisome, a Complex Nanomachine for Protein Injection into Mammalian Cells
Lara-Tejero M, Galán JE. The Injectisome, a Complex Nanomachine for Protein Injection into Mammalian Cells. EcoSal Plus 2019, 8: 10.1128/ecosalplus.esp-0039-2018. PMID: 30942149, PMCID: PMC6450406, DOI: 10.1128/ecosalplus.esp-0039-2018.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsEukaryotic CellsGram-Negative BacteriaHost-Pathogen InteractionsProtein TransportType III Secretion SystemsVirulence FactorsConceptsEffector proteinsGram-negative bacteriaSecretion systemBacterial envelopeType III protein secretion systemProtein secretion systemHost cellular functionsEukaryotic plasma membraneEukaryotic cell membranesHost cell cytosolMultiprotein nanomachineEukaryotic hostsComplex nanomachinesImportant bacterial pathogensCellular functionsMammalian cellsNeedle complexOuter membranePlasma membraneCell cytosolInjectisomeCell membraneProteinBacterial pathogensCentral roleOriganum vulgare L. essential oil inhibits the growth of carbapenem-resistant gram-negative bacteria
Vasconcelos N, Croda J, Silva K, Motta M, Maciel W, Limiere L, Simionatto S. Origanum vulgare L. essential oil inhibits the growth of carbapenem-resistant gram-negative bacteria. Revista Da Sociedade Brasileira De Medicina Tropical 2019, 52: e20180502. PMID: 31271619, DOI: 10.1590/0037-8682-0502-2018.Peer-Reviewed Original ResearchConceptsCarbapenem-resistant strainsAlternative therapeutic optionTime-kill assaysMultidrug-resistant bacteriaTherapeutic optionsMinimum inhibitory concentrationAcinetobacter baumanniiCell countCarbapenemase productionKill assaysOriganum vulgare L. essential oilL. essential oilKlebsiella pneumoniaeInhibitory concentrationDrug developmentResistant bacteriaH treatmentSerratia marcescensAntimicrobial effectNegative bacteria
2017
Prevalence, antibiotic susceptibility and characterization of antibiotic resistant genes among carbapenem-resistant Gram-negative bacilli and yeast in intestinal flora of cancer patients in North Lebanon
Christophy R, Osman M, Mallat H, Achkar M, Ziedeh A, Moukaddem W, Dabboussi F, Hamze M. Prevalence, antibiotic susceptibility and characterization of antibiotic resistant genes among carbapenem-resistant Gram-negative bacilli and yeast in intestinal flora of cancer patients in North Lebanon. Journal Of Infection And Public Health 2017, 10: 716-720. PMID: 28216021, DOI: 10.1016/j.jiph.2016.10.009.Peer-Reviewed Original ResearchConceptsCarbapenem-resistant bacteriaCancer patientsIntestinal carriageCarbapenem-resistant Gram-negative bacilliAntibiotic stewardship programsInfection control measuresDepartment of ChemotherapyGram-negative bacilliOXA-48 genePublic health concernAntimicrobial susceptibility profilesNini HospitalEndogenous infectionsMechanisms of resistanceStool samplesHigh prevalenceIntestinal floraHodge testStewardship programsPatientsAntibiotic susceptibilityNorth LebanonChemotherapySusceptibility profilesHealth concern
2016
Rampant Cheating by Pathogens?
Rundell EA, McKeithen-Mead SA, Kazmierczak BI. Rampant Cheating by Pathogens? PLOS Pathogens 2016, 12: e1005792. PMID: 27606630, PMCID: PMC5015830, DOI: 10.1371/journal.ppat.1005792.Peer-Reviewed Original ResearchAnimalsGenetic FitnessGram-Negative BacteriaGram-Negative Bacterial InfectionsHumansType III Secretion Systems
2015
Two Na+ Sites Control Conformational Change in a Neurotransmitter Transporter Homolog*
Tavoulari S, Margheritis E, Nagarajan A, DeWitt DC, Zhang YW, Rosado E, Ravera S, Rhoades E, Forrest LR, Rudnick G. Two Na+ Sites Control Conformational Change in a Neurotransmitter Transporter Homolog*. Journal Of Biological Chemistry 2015, 291: 1456-1471. PMID: 26582198, PMCID: PMC4714228, DOI: 10.1074/jbc.m115.692012.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SubstitutionAmino Acid Transport SystemsAquatic OrganismsBacterial ProteinsBinding SitesCysteineGram-Negative BacteriaLigandsLiposomesModels, MolecularMolecular Dynamics SimulationMutagenesis, Site-DirectedMutationPlasma Membrane Neurotransmitter Transport ProteinsProtein ConformationProtein FoldingProtein StabilityProteolipidsRecombinant ProteinsSodiumConceptsConformational changesTransmembrane helix 1Open conformational stateDependent conformational changesTransporter homologExtracellular gateProkaryotic homologCytoplasmic pathwayHelix 1Interaction networksIntermediary interactionsBiophysical assaysNeurotransmitter transportersSubstrate pathwayNa2 siteConformational statesHelix motionsLeuTDirect interactionDependent closureHomologMutantsDistinct stepsResiduesComputational analysisVaccination Serology Status and Cardiovascular Mortality: Insight from NHANES III and Continuous NHANES
Chothani A, Shah N, Patel NJ, Deshmukh A, Singh V, Patel N, Panaich SS, Arora S, Patel A, Savani C, Thakkar B, Bhatt P, Cohen MG, Grines C, Forrest JK, Badheka AO. Vaccination Serology Status and Cardiovascular Mortality: Insight from NHANES III and Continuous NHANES. Postgraduate Medicine 2015, 127: 561-564. PMID: 26174358, DOI: 10.1080/00325481.2015.1064300.Peer-Reviewed Original ResearchConceptsCV mortalityVaccination titersContinuous NHANESNHANES IIIProportional hazards regression modelingNutrition Examination Survey IIINon-influenza vaccinesExamination Survey IIINon-institutionalized patientsOverall health statusNHANES III dataSerology statusSeroprotective titersVaccination variablesCardiovascular eventsInfluenza vaccinationCardiovascular mortalityHazard ratioRoutine vaccinationNational HealthProtective effectHealth statusVaccinationReferent groupMortality
2014
Use of Imipenem To Detect KPC, NDM, OXA, IMP, and VIM Carbapenemase Activity from Gram-Negative Rods in 75 Minutes Using Liquid Chromatography-Tandem Mass Spectrometry
Kulkarni MV, Zurita AN, Pyka JS, Murray TS, Hodsdon ME, Peaper DR. Use of Imipenem To Detect KPC, NDM, OXA, IMP, and VIM Carbapenemase Activity from Gram-Negative Rods in 75 Minutes Using Liquid Chromatography-Tandem Mass Spectrometry. Journal Of Clinical Microbiology 2014, 52: 2500-2505. PMID: 24789180, PMCID: PMC4097713, DOI: 10.1128/jcm.00547-14.Peer-Reviewed Original Research
2013
Functional Polymorphisms in the Gene Encoding Macrophage Migration Inhibitory Factor Are Associated With Gram-Negative Bacteremia in Older Adults
Das R, Subrahmanyan L, Yang IV, van Duin D, Levy R, Piecychna M, Leng L, Montgomery RR, Shaw A, Schwartz DA, Bucala R. Functional Polymorphisms in the Gene Encoding Macrophage Migration Inhibitory Factor Are Associated With Gram-Negative Bacteremia in Older Adults. The Journal Of Infectious Diseases 2013, 209: 764-768. PMID: 24158957, PMCID: PMC3923543, DOI: 10.1093/infdis/jit571.Peer-Reviewed Original ResearchConceptsMacrophage migration inhibitory factorMigration inhibitory factorLow expressionOlder adultsInhibitory factorSurface Toll-like receptor 4Toll-like receptor 4Gram-negative bacteremiaPeripheral blood monocytesNegative bacterial infectionsMIF productionImmune mediatorsNegative bacteremiaHealthy controlsReceptor 4Blood monocytesOlder subjectsBacterial infectionsFunctional polymorphismsHigh expressionBacteremiaAdultsExpressionPatientsCohort
2012
Role of Catecholate Siderophores in Gram-Negative Bacterial Colonization of the Mouse Gut
Pi H, Jones S, Mercer L, Meador J, Caughron J, Jordan L, Newton S, Conway T, Klebba P. Role of Catecholate Siderophores in Gram-Negative Bacterial Colonization of the Mouse Gut. PLOS ONE 2012, 7: e50020. PMID: 23209633, PMCID: PMC3510177, DOI: 10.1371/journal.pone.0050020.Peer-Reviewed Original ResearchEnhancing tsetse fly refractoriness to trypanosome infection – A new IAEA coordinated research project
Van Den Abbeele J, Bourtzis K, Weiss B, Cordón-Rosales C, Miller W, Abd-alla AM, Parker A. Enhancing tsetse fly refractoriness to trypanosome infection – A new IAEA coordinated research project. Journal Of Invertebrate Pathology 2012, 112: s142-s147. PMID: 22841950, DOI: 10.1016/j.jip.2012.07.020.Peer-Reviewed Original ResearchMicrobial Spectrum and Resistance Patterns in Endophthalmitis: A 21-Year (1988–2008) Review in Northeast United States
Chen X, Adelman RA. Microbial Spectrum and Resistance Patterns in Endophthalmitis: A 21-Year (1988–2008) Review in Northeast United States. Journal Of Ocular Pharmacology And Therapeutics 2012, 28: 329-334. PMID: 22506856, DOI: 10.1089/jop.2011.0204.Peer-Reviewed Original Research
2011
Association of bacteria with hydrocephalus in Ugandan infants.
Li L, Padhi A, Ranjeva S, Donaldson S, Warf B, Mugamba J, Johnson D, Opio Z, Jayarao B, Kapur V, Poss M, Schiff S. Association of bacteria with hydrocephalus in Ugandan infants. Journal Of Neurosurgery Pediatrics 2011, 7: 73-87. PMID: 21194290, DOI: 10.3171/2010.9.peds10162.Peer-Reviewed Original ResearchConceptsUgandan infantsNeonatal sepsisPostinfectious hydrocephalusMajority of patientsMost patientsRecent infectionPolymerase chain reactionEffective treatmentPatientsPrevention strategiesHydrocephalusInfectionInfantsAcinetobacter speciesChain reactionAssociation of bacteriaBacterial DNAGram-negative bacteriaNegative bacteriaSepsisEnvironmental samplingSyndromeSeason infection
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