2023
Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson’s disease
Vidyadhara D, Somayaji M, Wade N, Yücel B, Zhao H, Shashaank N, Ribaudo J, Gupta J, Lam T, Sames D, Greene L, Sulzer D, Chandra S. Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson’s disease. Cell Reports 2023, 42: 112231. PMID: 36920906, PMCID: PMC10127800, DOI: 10.1016/j.celrep.2023.112231.Peer-Reviewed Original ResearchConceptsParkinson's diseaseMotor deficitsDopamine transporterPathogenesis of PDNigral dopaminergic lossProgressive motor deficitsΑ-synuclein pathologyEarly-onset Parkinson's diseaseDopamine sequestrationDopaminergic vulnerabilityDopaminergic lossKO micePresynaptic sitesKO brainNeurochemical analysisDorsal striatumKnockout miceCardinal featuresReuptake kineticsL-DOPADiseaseSynaptic autophagyFunction mutationsDeficitsMice
2022
Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain.
Massaro Tieze S, Chandra SS, Vidyadhara DJ. Subcellular Fractionation for the Isolation of Synaptic Components from the Murine Brain. Journal Of Visualized Experiments 2022 PMID: 36190269, PMCID: PMC10936730, DOI: 10.3791/64574.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainCell FractionationMiceSubcellular FractionsSynaptic MembranesSynaptic VesiclesSynaptosomesConceptsSubcellular fractionationPost-synaptic compartmentsBiochemical isolationSub-synaptic compartmentsSynaptic cytosolPlasma membraneMolecular basisEnrichment analysisNeuronal preMolecular etiologySynaptic vesiclesSynaptic terminalsSynaptic plasma membranesSynaptic componentsBrain tissue homogenizationSynaptic terminal structureTerminal structureActive stateNeuronal communicationMurine brainSynaptic structureFunctional characteristicsMouse brainHypotonic lysisSynaptic dysfunction