2016
Molecular organization of cytokinesis nodes and contractile rings by super-resolution fluorescence microscopy of live fission yeast
Laplante C, Huang F, Tebbs IR, Bewersdorf J, Pollard TD. Molecular organization of cytokinesis nodes and contractile rings by super-resolution fluorescence microscopy of live fission yeast. Proceedings Of The National Academy Of Sciences Of The United States Of America 2016, 113: e5876-e5885. PMID: 27647921, PMCID: PMC5056082, DOI: 10.1073/pnas.1608252113.Peer-Reviewed Original ResearchConceptsFluorescence photoactivation localization microscopyContractile ringLive fission yeast cellsLive fission yeastFission yeast cellsCytokinetic contractile ringSuper-resolution fluorescence microscopyCytokinesis nodesPhotoactivation localization microscopyFission yeastFormin Cdc12pActin networkIQ motifPlasma membraneCytokinesisConstituent proteinsMyosin-II tailYeast cellsActin ringsActin strandsAssembly precursorsDiscrete structural unitsFluorescence microscopyMolecular organizationProteinAvoiding artefacts when counting polymerized actin in live cells with LifeAct fused to fluorescent proteins
Courtemanche N, Pollard TD, Chen Q. Avoiding artefacts when counting polymerized actin in live cells with LifeAct fused to fluorescent proteins. Nature Cell Biology 2016, 18: 676-683. PMID: 27159499, PMCID: PMC5509211, DOI: 10.1038/ncb3351.Peer-Reviewed Original Research
2014
Local and global analysis of endocytic patch dynamics in fission yeast using a new “temporal superresolution” realignment method
Berro J, Pollard TD. Local and global analysis of endocytic patch dynamics in fission yeast using a new “temporal superresolution” realignment method. Molecular Biology Of The Cell 2014, 25: 3501-3514. PMID: 25143395, PMCID: PMC4230612, DOI: 10.1091/mbc.e13-01-0004.Peer-Reviewed Original ResearchConceptsFission yeastEndocytic actin patchesWild-type cellsEndocytic patchesActin patchesQuantitative microscopyActin assemblyCellular processesVesicle movementEndocytic vesiclesInterphase cellsVesicle formationMolecular mechanismsPatch dynamicsYeastCell lengthGlobal analysisNumber of patchesMicroscopy moviesCellsClathrinEndocytosisNew toolValuable toolPatchesMechanism of Cytokinetic Contractile Ring Constriction in Fission Yeast
Stachowiak MR, Laplante C, Chin HF, Guirao B, Karatekin E, Pollard TD, O’Shaughnessy B. Mechanism of Cytokinetic Contractile Ring Constriction in Fission Yeast. Developmental Cell 2014, 29: 547-561. PMID: 24914559, PMCID: PMC4137230, DOI: 10.1016/j.devcel.2014.04.021.Peer-Reviewed Original Research
2011
Distinct Roles for F-BAR Proteins Cdc15p and Bzz1p in Actin Polymerization at Sites of Endocytosis in Fission Yeast
Arasada R, Pollard TD. Distinct Roles for F-BAR Proteins Cdc15p and Bzz1p in Actin Polymerization at Sites of Endocytosis in Fission Yeast. Current Biology 2011, 21: 1450-1459. PMID: 21885283, PMCID: PMC3350781, DOI: 10.1016/j.cub.2011.07.046.Peer-Reviewed Original ResearchConceptsF-BAR proteinsNucleation-promoting factorsActin patchesFission yeastArp2/3 complexActin polymerizationActin filamentsGenetic interaction experimentsSites of endocytosisPeripheral membrane proteinsActin binding proteinsClathrin-mediated endocytosisMembrane scissionYeast showSH3 domainCdc15pMembrane proteinsMembrane tubulesPlasma membraneGenetic analysisBinding proteinDistinct rolesEndocytosisProteinClathrin
2007
Assembly Mechanism of the Contractile Ring for Cytokinesis by Fission Yeast
Vavylonis D, Wu JQ, Hao S, O'Shaughnessy B, Pollard TD. Assembly Mechanism of the Contractile Ring for Cytokinesis by Fission Yeast. Science 2007, 319: 97-100. PMID: 18079366, DOI: 10.1126/science.1151086.Peer-Reviewed Original ResearchConceptsContractile ringActin filamentsLive fission yeast cellsIndividual daughter cellsFission yeast cellsDynamic actin filamentsFission yeastMotor protein myosinCell equatorDaughter cellsYeast cellsAssembly mechanismProtein myosinFluorescence microscopyCytokinesisMyosinFilamentsTransient connectionsCellsYeastFungiMechanismMeshwork