Mixed-culture assays for analyzing neuronal synapse formation
Biederer T, Scheiffele P. Mixed-culture assays for analyzing neuronal synapse formation. Nature Protocols 2007, 2: 670-676. PMID: 17406629, DOI: 10.1038/nprot.2007.92.Peer-Reviewed Original ResearchConceptsVertebrate central nervous systemIndividual cell surface proteinsSynaptic adhesion complexesCell biological mechanismsPostsynaptic membrane domainsCell surface proteinsAssembly of synapsesAdhesion complexesMembrane domainsNeuronal synapse formationIndividual proteinsSynaptogenic activityGenetic studiesBidirectional signalingPrimary neuronal culturesSynapse formationSynaptic differentiationMixed culture assayBiological mechanismsSynaptic structureProteinNew insightsSynaptic cleftNeuronal culturesAssay system
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