Nikolay G Kolev, PhD
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Epidemiology of Microbial Diseases
BCMM 137, 295 Congress Ave
New Haven, CT 06519
United States
About
Titles
Research Scientist in Epidemiology (Microbial Diseases)
Biography
Nikolay Kolev earned his PhD in Biochemistry from the University of Notre Dame. He was then a postdoctoral associate in the Department of Molecular Biophysics and Biochemistry at Yale University. Together with Dr. Christian Tschudi, he studies various aspects of RNA metabolism in African trypanosomes. The primary focus of Dr. Kolev’s research is the molecular mechanism governing the development of the protozoan parasite Trypanosoma brucei to its infectious metacyclic form, responsible for the transmission of African sleeping sickness and related diseases from the tsetse fly vector to the mammalian host.
Appointments
Epidemiology of Microbial Diseases
Research ScientistPrimary
Other Departments & Organizations
- Epidemiology of Microbial Diseases
- Yale School of Public Health
Education & Training
- Postdoctoral Associate
- HHMI, Yale University (2008)
- PhD
- University of Notre Dame (2003)
- MS
- University of Sofia (1997)
Research
Overview
Medical Subject Headings (MeSH)
Antigenic Variation; Gene Expression Regulation; Parasites; Transcriptional Activation; Trypanosoma; Trypanosomiasis, African
Research at a Glance
Yale Co-Authors
Frequent collaborators of Nikolay G Kolev's published research.
Publications Timeline
A big-picture view of Nikolay G Kolev's research output by year.
Research Interests
Research topics Nikolay G Kolev is interested in exploring.
Christian Tschudi, PhD
Joan Steitz, PhD
Kazimierz Tycowski, PhD
25Publications
1,236Citations
Gene Expression Regulation
Trypanosomiasis, African
Trypanosoma
Parasites
Publications
2019
The vault RNA of Trypanosoma brucei plays a role in the production of trans-spliced mRNA
Kolev NG, Rajan KS, Tycowski KT, Toh JY, Shi H, Lei Y, Michaeli S, Tschudi C. The vault RNA of Trypanosoma brucei plays a role in the production of trans-spliced mRNA. Journal Of Biological Chemistry 2019, 294: 15559-15574. PMID: 31439669, PMCID: PMC6816085, DOI: 10.1074/jbc.ra119.008580.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsTelomerase-associated protein 1Vault RNAY RNAsRNA quality controlRNA polymerase IIISmall nuclear RNATrans-spliced mRNAPermeabilized cell systemNcRNA repertoireRNP biogenesisMRNA metabolismRNA speciesSequence similarityRNA classesMammalian cellsNuclear RNAPolymerase IIIRo functionTrypanosoma bruceiVtRNAsGene expressionRo proteinBloodstream formsRNACell nuclei
2018
Temperature shift activates bloodstream VSG expression site promoters in Trypanosoma brucei
Kolev NG, Ramsdell TK, Tschudi C. Temperature shift activates bloodstream VSG expression site promoters in Trypanosoma brucei. Molecular And Biochemical Parasitology 2018, 226: 20-23. PMID: 30399391, PMCID: PMC6258174, DOI: 10.1016/j.molbiopara.2018.10.003.Peer-Reviewed Original ResearchCitationsAltmetric
2017
The proteome and transcriptome of the infectious metacyclic form of Trypanosoma brucei define quiescent cells primed for mammalian invasion
Christiano R, Kolev NG, Shi H, Ullu E, Walther TC, Tschudi C. The proteome and transcriptome of the infectious metacyclic form of Trypanosoma brucei define quiescent cells primed for mammalian invasion. Molecular Microbiology 2017, 106: 74-92. PMID: 28742275, PMCID: PMC5607103, DOI: 10.1111/mmi.13754.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsInfectious metacyclic formsTsetse fly vectorMetacyclic formsVariant surface glycoprotein expression sitesQuiescent cellsGene expression profilesFly vectorsAvailability of nutrientsMammalian invasionsCell surface componentsCell divisionProcyclic trypanosomesMammalian hostsTrypanosoma bruceiMRNA sequencingExpression profilesExpression sitesMetabolic enzymesBloodstream formsInfectious metacyclicsProtein 6TranscriptomeTsetse fliesProteomeProtein levelsMetacyclic VSG expression site promoters are recognized by the same general transcription factor that is required for RNA polymerase I transcription of bloodstream expression sites
Kolev NG, Günzl A, Tschudi C. Metacyclic VSG expression site promoters are recognized by the same general transcription factor that is required for RNA polymerase I transcription of bloodstream expression sites. Molecular And Biochemical Parasitology 2017, 216: 52-55. PMID: 28716719, PMCID: PMC5582990, DOI: 10.1016/j.molbiopara.2017.07.002.Peer-Reviewed Original ResearchCitationsMeSH Keywords and ConceptsConceptsBloodstream VSG expression sitesVSG expression sitesVSG ES promoterExpression sitesPol IVSG genesES promoterRNA polymerase I transcriptionGeneral transcription factorsBloodstream expression sitesVariant surface glycoprotein (VSG) coatPolymerase I transcriptionRNA polymerase IDistinct promoter sequencesMetacyclic VSG geneTrypanosoma brucei cellsProcyclin genesTypes of promotersBrucei cellsDevelopmental programDistinct binding affinitiesTranscription factorsI transcriptionInitiation factorsPromoter sequencesThe Canonical Poly (A) Polymerase PAP1 Polyadenylates Non-Coding RNAs and Is Essential for snoRNA Biogenesis in Trypanosoma brucei
Chikne V, Gupta SK, Doniger T, K. SR, Cohen-Chalamish S, Ben-Asher HW, Kolet L, Yahia NH, Unger R, Ullu E, Kolev NG, Tschudi C, Michaeli S. The Canonical Poly (A) Polymerase PAP1 Polyadenylates Non-Coding RNAs and Is Essential for snoRNA Biogenesis in Trypanosoma brucei. Journal Of Molecular Biology 2017, 429: 3301-3318. PMID: 28456523, DOI: 10.1016/j.jmb.2017.04.015.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsNon-coding RNAsPoly (ADP-ribose) polymeraseUnique RNA processing mechanismsCis-spliced intronsRNA processing mechanismsSmall nucleolar RNAsLong non-coding RNAsPolyadenylation of mRNAParasite Trypanosoma bruceiSnoRNA biogenesisTrans splicingPrecursor snoRNASnoRNA processingNucleolar RNAsPAP1Trypanosoma bruceiPolyadenylationMajor substrateSnoRNAsTrypanosoma cruziPAP2RNAPolymerase Pap1TrypanosomesCausative agent
2016
Transcriptome Profiling of Trypanosoma brucei Development in the Tsetse Fly Vector Glossina morsitans
Savage AF, Kolev NG, Franklin JB, Vigneron A, Aksoy S, Tschudi C. Transcriptome Profiling of Trypanosoma brucei Development in the Tsetse Fly Vector Glossina morsitans. PLOS ONE 2016, 11: e0168877. PMID: 28002435, PMCID: PMC5176191, DOI: 10.1371/journal.pone.0168877.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsMeSH KeywordsAnimalsElectron Transport Complex IVGene Expression ProfilingGene LibraryHigh-Throughput Nucleotide SequencingInsect VectorsIntestinal MucosaLife Cycle StagesMembrane GlycoproteinsProtozoan ProteinsProventriculusSalivary GlandsSequence Analysis, RNATranscriptomeTrypanosoma brucei bruceiTsetse FliesUp-RegulationConceptsSalivary gland transcriptomeMetacyclic VSGsInsect vectorsHigh-throughput RNA sequencingBlood-feeding tsetse fliesDigenetic life cycleRNA-binding proteinGene ontology analysisAlanine-rich proteinCytochrome oxidase complexTsetse fliesMidgut transcriptomeGlobal regulatorDNA metabolismTrypanosome developmentOntology analysisDNA replicationSignal transductionTransporter familyMammalian hostsRNA sequencingDistinct tissuesTranscript levelsTranscriptomeRich protein
2015
Genome-wide analysis of small nucleolar RNAs of Leishmania major reveals a rich repertoire of RNAs involved in modification and processing of rRNA
Eliaz D, Doniger T, Tkacz ID, Biswas VK, Gupta SK, Kolev NG, Unger R, Ullu E, Tschudi C, Michaeli S. Genome-wide analysis of small nucleolar RNAs of Leishmania major reveals a rich repertoire of RNAs involved in modification and processing of rRNA. RNA Biology 2015, 12: 1222-1255. PMID: 25970223, PMCID: PMC4829279, DOI: 10.1080/15476286.2015.1038019.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsSmall nucleolar RNAsNucleolar RNAsGenome-wide analysisRNA processing mechanismsRRNA secondary structurePost-transcriptional levelCore proteinProcessing of rRNARNA-seq analysisFunctional homologueGene clusterRNA speciesTrypanosoma bruceiSnoRNAsComplete repertoireGene expressionRRNA cleavageAnalogous functionsSecondary structureRNAProtozoan parasiteRich repertoireOrganismsSpeciesProteinSynchronous expression of individual metacyclic variant surface glycoprotein genes in Trypanosoma brucei
Ramey-Butler K, Ullu E, Kolev NG, Tschudi C. Synchronous expression of individual metacyclic variant surface glycoprotein genes in Trypanosoma brucei. Molecular And Biochemical Parasitology 2015, 200: 1-4. PMID: 25896436, PMCID: PMC4470760, DOI: 10.1016/j.molbiopara.2015.04.001.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsVariant surface glycoproteinMetacyclic variant surface glycoproteinInfectious metacyclic formsVariant surface glycoprotein genesVSG gene expressionSurface glycoprotein genesNew mammalian hostInsect vectorsParasite biologyMammalian hostsDifferentiation systemGene expressionTsetse vectorBloodstream trypanosomesDifferential expressionGlycoprotein geneProtein 6Molecular aspectsIndividual cellsMetacyclic formsBlood mealProtein levelsDiscrete populationsSurface glycoproteinLife cycle
2014
Construction of Trypanosoma brucei Illumina RNA-Seq Libraries Enriched for Transcript Ends
Kolev NG, Ullu E, Tschudi C. Construction of Trypanosoma brucei Illumina RNA-Seq Libraries Enriched for Transcript Ends. Methods In Molecular Biology 2014, 1201: 165-175. PMID: 25388113, DOI: 10.1007/978-1-4939-1438-8_9.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsHuman pathogen Trypanosoma bruceiHigh-throughput RNA sequencingLong polycistronic precursorsGenome-wide scaleIllumina RNA-seqPathogen Trypanosoma bruceiRNA polymerase IIRNA-seq librariesSingle-nucleotide resolutionTranscript endsTranscript boundariesTranscriptome annotationUnannotated genesPolymerase IICellular abundancePolycistronic precursorsTranscription initiationGene clusterRNA-seqPolyadenylation sitesRNA sequencingMRNA precursorsTrypanosoma bruceiRNA moleculesMRNA moleculesThe emerging role of RNA‐binding proteins in the life cycle of Trypanosoma brucei
Kolev NG, Ullu E, Tschudi C. The emerging role of RNA‐binding proteins in the life cycle of Trypanosoma brucei. Cellular Microbiology 2014, 16: 482-489. PMID: 24438230, PMCID: PMC3974610, DOI: 10.1111/cmi.12268.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsParasitic protozoan Trypanosoma bruceiIdentification of RBPsTargets of RBPsSpecific gene networksProtozoan Trypanosoma bruceiPost-transcriptional mechanismsGene regulatory networksGene expression patternsRelated trypanosomatidsTranscriptional machineryCellular fateGene networksRegulatory networksTrypanosoma bruceiExpression patternsRegulatory mechanismsDifferent hostsRBPsLife cyclePathogenic organismsRNAOrganismsProteinDifferent environmentsCrucial role
Academic Achievements and Community Involvement
honor Molecular, Cellular and Immunoparasitology Scientific Award in recognition of scientific excellence
National AwardThe American Society of Tropical Medicine and Hygiene (ASTMH)Details09/15/2009United States
Links & Media
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vtRNA of Trypanosoma brucei
The vault RNA (vtRNA) of Trypanosoma brucei, the unicellular eukaryote causing African sleeping sickness, is shown set against an image of live procyclic form parasites in this cover photo for the October 25, 2019 issue of the Journal of Biological Chemistry.
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Epidemiology of Microbial Diseases
BCMM 137, 295 Congress Ave
New Haven, CT 06519
United States
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Boyer Center for Molecular Medicine
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