2016
The Listeria monocytogenes Fur‐regulated virulence protein FrvA is an Fe(II) efflux P1B4‐type ATPase
Pi H, Patel S, Argüello J, Helmann J. The Listeria monocytogenes Fur‐regulated virulence protein FrvA is an Fe(II) efflux P1B4‐type ATPase. Molecular Microbiology 2016, 100: 1066-1079. PMID: 26946370, PMCID: PMC4914386, DOI: 10.1111/mmi.13368.Peer-Reviewed Original Research
2014
Structure and Cell Wall Cleavage by Modular Lytic Transglycosylase MltC of Escherichia coli
Artola-Recolons C, Lee M, Bernardo-García N, Blázquez B, Hesek D, Bartual S, Mahasenan K, Lastochkin E, Pi H, Boggess B, Meindl K, Usón I, Fisher J, Mobashery S, Hermoso J. Structure and Cell Wall Cleavage by Modular Lytic Transglycosylase MltC of Escherichia coli. ACS Chemical Biology 2014, 9: 2058-2066. PMID: 24988330, PMCID: PMC4168783, DOI: 10.1021/cb500439c.Peer-Reviewed Original ResearchConceptsTwo-domain structureLytic transglycosylasesCell wall assemblyEscherichia coliEssential bacterial enzymeResolution X-ray structureÅ resolution X-ray structureGram-negative bacterium Escherichia coliBacterium Escherichia coliBacterial cell wallCell wall analoguesBacterial genomesGlycan strandsGenomic analysisActive siteCell wallBacterial enzymesFunctional roleSoluble constructWall assemblyGenomeTransglycosylasesExolytic cleavageX-ray structurePeptidoglycan
2012
A Putative P-Type ATPase Required for Virulence and Resistance to Haem Toxicity in Listeria monocytogenes
McLaughlin H, Xiao Q, Rea R, Pi H, Casey P, Darby T, Charbit A, Sleator R, Joyce S, Cowart R, Hill C, Klebba P, Gahan C. A Putative P-Type ATPase Required for Virulence and Resistance to Haem Toxicity in Listeria monocytogenes. PLOS ONE 2012, 7: e30928. PMID: 22363518, PMCID: PMC3283593, DOI: 10.1371/journal.pone.0030928.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphatasesAnimalsBacterial ProteinsBase SequenceBiological AssayComputational BiologyDrug Resistance, BacterialGene Expression Regulation, BacterialGenes, BacterialGenetic LociHemeHomeostasisIronListeria monocytogenesListeriosisMiceMolecular Sequence DataMothsMutagenesis, InsertionalMutationPlasmidsReal-Time Polymerase Chain ReactionRepressor ProteinsVirulenceConceptsPutative P-type ATPaseFerric uptake regulatorUptake of haemIntracellular pathogen Listeria monocytogenesWax moth Galleria mellonellaP-type ATPaseAcquisition of ironPathogen Listeria monocytogenesMoth Galleria mellonellaIron-deficient mediumMembrane proteinsMutantsHaem toxicityFree haemAlternative infection modelGalleria mellonellaIron homeostasisInhibition of growthFrvAVirulenceListeria monocytogenesHaemVirulence potentialFurDeficient medium