2014
Intra-Subtype Variation in Enteroadhesion Accounts for Differences in Epithelial Barrier Disruption and Is Associated with Metronidazole Resistance in Blastocystis Subtype-7
Wu Z, Mirza H, Tan KS. Intra-Subtype Variation in Enteroadhesion Accounts for Differences in Epithelial Barrier Disruption and Is Associated with Metronidazole Resistance in Blastocystis Subtype-7. PLOS Neglected Tropical Diseases 2014, 8: e2885. PMID: 24851944, PMCID: PMC4031124, DOI: 10.1371/journal.pntd.0002885.Peer-Reviewed Original ResearchConceptsEpithelial barrier disruptionBarrier disruptionDrug resistanceBlastocystis subtype 7Intra-subtype variationOccludin tight junction proteinsEpithelial barrier dysfunctionDiverse clinical outcomesParasite virulence factorsTight junction proteinsCaco-2 cellsClinical outcomesBarrier dysfunctionIntestinal disordersMetronidazole resistanceBlastocystis infectionSubtype 7Metronidazole susceptibilityHost responseIntestinal epitheliumEnteric pathogensNitrosative stressResistant strainsJunction proteinsLuminal stages
2012
Blastocystis–Host Interactions: Insights from In Vitro Model Systems
Tan K, Mirza H. Blastocystis–Host Interactions: Insights from In Vitro Model Systems. Parasitology Research Monographs 2012, 4: 51-63. DOI: 10.1007/978-3-642-32738-4_4.ChaptersIntra-subtype variationIntestinal epithelial cell lineHost immune responseProinflammatory cytokinesEpithelial cell lineINOS productionImmune responseBarrier compromiseVitro model systemAnimal modelsClinical relevanceBlastocystis subtypesCell linesPathogenesisVariety of effectsCell deathBlastocystisInsert systemParasitesCytokinesSubtypesImmunoglobulinPathobiology
2008
Blastocystis exhibits inter- and intra-subtype variation in cysteine protease activity
Mirza H, Tan KS. Blastocystis exhibits inter- and intra-subtype variation in cysteine protease activity. Parasitology Research 2008, 104: 355-361. PMID: 18846388, DOI: 10.1007/s00436-008-1203-1.Peer-Reviewed Original ResearchConceptsCysteine protease activityIntra-subtype variationRodent isolatesIL-8 responseColonic epithelial cellsHuman secretory IgAClinical studiesSecretory IgAAnimal modelsNon-virulent strainsProtease activityBlastocystis subtypesCysteine proteasesEpithelial cellsGiardia intestinalisCellular mechanismsZoonotic potentialBlastocystisEntamoeba histolyticaAvian isolatesPresent studyIsolatesSame isolatesParasitesIgA