Voluntary Faculty
Voluntary faculty are typically clinicians or others who are employed outside of the School but make significant contributions to department programs at the medical center or at affiliate institutions.
Voluntary rank detailsEdward Ryan, PhD
Associate Clinical ProfessorAbout
Research
Publications
2025
Human antibody targeting Vibrio cholerae O1 O-specific polysaccharide induces an amotile hypovirulent bacterial phenotype: mechanism of protection against cholera.
Verma S, Cetinbas M, Kelly M, Senger S, Faherty C, Janardhanan J, Wagh C, Bhuiyan T, Chowdhury F, Khan A, Akter A, Charles R, Harris J, Calderwood S, Wrammert J, Waldor M, Asp M, Tai J, Yan J, Xu P, Kováč P, Sadreyev R, Qadri F, Ryan E. Human antibody targeting Vibrio cholerae O1 O-specific polysaccharide induces an amotile hypovirulent bacterial phenotype: mechanism of protection against cholera. MBio 2025, e0223525. PMID: 40937849, DOI: 10.1128/mbio.02235-25.Peer-Reviewed Original ResearchAnti-OSP antibodiesO-specific polysaccharideVibrio cholerae,Bacterial motilityLevels of cyclic di-GMPInhibition of bacterial motilityEnteroid modelCyclic di-GMPCholera toxin detectionIntestinal surfaceCulture-based assaysInhibition of motilityVirulence programInfect humansRegulon expressionDi-GMPVirulence genesVirulence mechanismsBacterial phenotypesPathogen physiologyPresence of mucinExtracellular matrix componentsBiofilm formationBacterial metabolismIntestinal mucusGenomic insights into a diarrheal outbreak in Bangladesh reveal novel ETEC lineages and expansion of CS23 colonization factor.
Rahman S, Jubair M, Akhtar M, Akter A, Tauheed I, Begum Y, Bhattacharjee P, Afrad M, Khanam F, Islam M, Khan A, Rahman M, Ryan E, Fleckenstein J, Bhuiyan T, Thomson N, Qadri F, von Mentzer A, Chowdhury F. Genomic insights into a diarrheal outbreak in Bangladesh reveal novel ETEC lineages and expansion of CS23 colonization factor. Microbiology Spectrum 2025, e0331524. PMID: 40928300, DOI: 10.1128/spectrum.03315-24.Peer-Reviewed Original ResearchColonization factorsETEC strainsNovel lineagesGenomic surveillanceDiarrheal outbreaksETEC-associated diarrheaWhole-genome sequencingEscherichia coliETEC isolatesETEC infectionGenomic attributesGenomic diversityETEC toxinsGenome sequenceGenomic insightsPhylogenetic analysisVirulence profilesPopulation structureVirulence factorsAMR genesCause of diarrheaETECEvolutionary changesDiarrheal casesResistance mechanismsEvaluation of Cholera Antigen-Specific Gut-Homing β7-Positive Antibody-Secreting Cells in the Systemic Circulation of Oral Cholera Vaccinees Receiving Doses at Different Intervals
Karmakar P, Rashu R, Hoq M, Salma U, Islam K, Jahan N, Nishat N, Akter A, Jahan R, Dash P, Saha A, Ryan E, Qadri F, Bhuiyan T. Evaluation of Cholera Antigen-Specific Gut-Homing β7-Positive Antibody-Secreting Cells in the Systemic Circulation of Oral Cholera Vaccinees Receiving Doses at Different Intervals. Vaccines 2025, 13: 919. PMID: 41012122, PMCID: PMC12474143, DOI: 10.3390/vaccines13090919.Peer-Reviewed Original ResearchTwo-dose regimenAntibody-secreting cellsOral cholera vaccineT cell responsesSingle doseSystemic circulationDoses of oral cholera vaccineSingle dose of oral cholera vaccineSingle-dose strategyTwo-dose groupSingle dose of vaccineSingle-dose vaccinationDose of vaccineYears of ageCytotoxic memoryDay 7 post-vaccinationGut-homingGut homingCholera-endemic regionsImmune responseShancholShort-term protectionAdult volunteersGlobal health challengeRegimenVibrio cholerae-specific antibodies in plasma and saliva in cholera patients during a severe outbreak in Zambia: an antibody profiling approach
Luchen C, Ngo’mbe H, Liswaniso F, Bosomprah S, Zhiteneva J, Harris J, Charles R, Ryan E, Sack D, Bernshtein B, Chisenga C. Vibrio cholerae-specific antibodies in plasma and saliva in cholera patients during a severe outbreak in Zambia: an antibody profiling approach. Frontiers In Immunology 2025, 16: 1641319. PMID: 40895561, PMCID: PMC12394519, DOI: 10.3389/fimmu.2025.1641319.Peer-Reviewed Original ResearchConceptsOgawa O-specific polysaccharideElevated biomarker levelsResource-limited settingsNon-invasive alternativeAntibody profilesLevels of total IgGVaccine immunogenicity studiesTreatment centresMucosal immune responsesTotal IgG levelsPlasma-based assaysCholera toxin BIgG1-3Resource-constrained settingsIgG levelsMucosal pathogensPublic health threatMucosal immunityAntibody-based profilingImmunogenicity studiesImmune responseTotal IgGSecretory IgASalivary responseImmunological differencesEvaluation of colonization factors of ETEC isolated from diarrheal patients in Bangladesh: A comparative approach using multiplex PCR and dot blot immunoassay
Amin M, Akhtar M, Afrad M, Rahman S, Parvin N, Akter A, Tauheed I, Amin M, Ryan E, Khan A, Chowdhury F, Bhuiyan T, Begum Y, Qadri F. Evaluation of colonization factors of ETEC isolated from diarrheal patients in Bangladesh: A comparative approach using multiplex PCR and dot blot immunoassay. Journal Of Microbiological Methods 2025, 237: 107215. PMID: 40774609, DOI: 10.1016/j.mimet.2025.107215.Peer-Reviewed Original ResearchConceptsEnterotoxigenic Escherichia coliEnterotoxigenic Escherichia coli strainsColonization factorsMultiplex PCR assayDiarrheal patientsPCR assayDot blot assayDot blot methodMultiplex PCREnterotoxigenic Escherichia coli isolatesETEC colonization factorsPCR methodHeat-stable toxinMultiplex PCR methodHeat-labile toxinBlot assayDot blot immunoassayHospitalized diarrheal patientsBlot methodBlot immunoassayDot blotCFA/IHeat-labileCS21PCRAn O-Specific Polysaccharide Shigella flexneri 3a Conjugate Vaccine is Immunogenic and Protective against Virulent Keratoconjunctival Challenge in Guinea Pigs.
Janardhanan J, Wagh C, Yang J, Charles R, Pansuriya R, Chowdhury F, Kaminski R, Khan A, Bhuiyan T, Qadri F, Kováč P, Xu P, Ryan E. An O-Specific Polysaccharide Shigella flexneri 3a Conjugate Vaccine is Immunogenic and Protective against Virulent Keratoconjunctival Challenge in Guinea Pigs. American Journal Of Tropical Medicine And Hygiene 2025 PMID: 40763727, DOI: 10.4269/ajtmh.25-0269.Peer-Reviewed Original ResearchO-specific polysaccharideGuinea pigsConjugate vaccineVirulent strainsFunctional antibody responsesShigella sppResource-limited settingsShigella infectionShigellaImmunoglobulin (Ig)GImmune responseAnimal modelsAntibody responseIgM responseShigellosisVaccinePhysical stuntingGuineaYoung childrenVirulencePigsSppAnimalsDiarrheaSerumMosquito-Borne Infections in International Travelers.
Rolfe R, Zavala S, Blackwood E, LaRocque R, Ryan E. Mosquito-Borne Infections in International Travelers. Wilderness And Environmental Medicine 2025, 10806032251356485. PMID: 40686432, DOI: 10.1177/10806032251356485.Peer-Reviewed Original ResearchMosquito-borne infectionArboviral infectionsUS Food and Drug AdministrationDegree of suspicionZika virus infectionFood and Drug AdministrationMosquito-borne arboviral infectionFrequency of infectionDrug AdministrationVirus infectionMedical literatureInfectionNarrative reviewMalariaJapanese encephalitisMosquito-borne illnessesFeverDengue feverVaccineHealthcare providersInternational travelInsect repellentChemoprophylaxisIllnessYellow feverPulmonary function and comparative SARS-CoV-2 RBD-specific IgG antibody response among the COVID-19 recovered group
Siddik A, Faisal A, khan A, Alam M, Nayeem J, Kulsum U, Mukta S, Kawser Z, Hasan I, Azad K, Hossain M, Kar S, Sultana N, Alam R, Mustafa A, Habib M, Ryan E, Qadri F, Hassan M. Pulmonary function and comparative SARS-CoV-2 RBD-specific IgG antibody response among the COVID-19 recovered group. PLOS ONE 2025, 20: e0318959. PMID: 40644485, PMCID: PMC12250562, DOI: 10.1371/journal.pone.0318959.Peer-Reviewed Original ResearchConceptsForced Vital CapacityPulmonary function testsDose of vaccineIgG antibody responsesPulmonary functionAntibody titersInfection historyBooster doseFollow-upRecovered groupAntibody responseLong-term pulmonary consequencesProspective case control studyPulmonary function abnormalitiesControl groupAbnormal pulmonary functionCase-control studyLong-term respiratory healthHigh IgG antibody titersIgG antibody titersForced expiratory volumeAsymptomatic COVID-19Pulmonary function measurementsManifestations of long COVIDThird dosePrevalent chromosome fusion in Vibrio cholerae O1
Cuénod A, Chac D, Khan A, Chowdhury F, Hyppa R, Markiewicz S, Rice A, Kholwadwala A, Calderwood S, Ryan E, Harris J, LaRocque R, Bhuiyan T, Smith G, Qadri F, Lypaczewski P, Weil A, Shapiro B. Prevalent chromosome fusion in Vibrio cholerae O1. Nature Communications 2025, 16: 5830. PMID: 40593602, PMCID: PMC12219848, DOI: 10.1038/s41467-025-60699-0.Peer-Reviewed Original ResearchConceptsBacterial family VibrionaceaeChromosome fusion eventsLong-read sequencingV. cholerae growthVirulence factor expressionPathogen Vibrio choleraeV. cholerae O1 isolatesReports of isolationCircular chromosomeFamily VibrionaceaeVibrio cholerae O1Chromosome fusionsHomologous sequencesFused chromosomesKilobase-pairsFusion eventsO1 isolatesVibrio choleraeBiofilm formationChromosome stabilityChromosomeCholerae O1Detectable effectSequenceIsolatesEconomic value of pre-travel health interventions for communicable diseases in international travellers
Stanic T, Koiso S, Fields N, Walker A, Mulroy N, Ryan E, LaRocque R, Hyle E. Economic value of pre-travel health interventions for communicable diseases in international travellers. Journal Of Travel Medicine 2025, taaf053. PMID: 40581744, DOI: 10.1093/jtm/taaf053.Peer-Reviewed Original ResearchPre-travel interventionsClinical outcomesGroups of travelersInternational travelTraveler's diarrheaHealth interventionsRisk perceptionCost-effectiveness analysisCommunicable diseasesEvidence baseLiterature reviewTravel purposesTravel destinationHepatitis A vaccineEconomic valueMeasles-mumps-rubella vaccineEconomic evaluation studiesTravelPrevention of hepatitis AMalaria chemoprophylaxisRisk of transmissionHepatitis BLiterature review of studiesCost-effectiveHealth policy
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