Chunxiang Wu
Associate Research ScientistAbout
Research
Publications
2025
Distinct Target Site of Lenacapavir in Immature HIV‑1 and Concurrent Binding with the Maturation Inhibitor Bevirimat
Wu C, Meuser M, Rey J, Meshkin H, Yang R, Devarkar S, Freniere C, Shi J, Aiken C, Perilla J, Xiong Y. Distinct Target Site of Lenacapavir in Immature HIV‑1 and Concurrent Binding with the Maturation Inhibitor Bevirimat. Journal Of The American Chemical Society 2025, 147: 42685-42700. PMID: 41198571, DOI: 10.1021/jacs.5c13735.Peer-Reviewed Original ResearchExploring the Structural Divergence of HIV and SRLV Lentiviral Capsids
Arizaga F, Freniere C, Rey J, Cook M, Wu C, Perilla J, Xiong Y. Exploring the Structural Divergence of HIV and SRLV Lentiviral Capsids. Journal Of The American Chemical Society 2025, 147: 32883-32895. PMID: 40878534, PMCID: PMC12515838, DOI: 10.1021/jacs.5c09436.Peer-Reviewed Original ResearchStructural insights into HIV-2 CA lattice formation and FG-pocket binding revealed by single-particle cryo-EM
Cook M, Freniere C, Wu C, Lozano F, Xiong Y. Structural insights into HIV-2 CA lattice formation and FG-pocket binding revealed by single-particle cryo-EM. Cell Reports 2025, 44: 115245. PMID: 39864060, PMCID: PMC11912512, DOI: 10.1016/j.celrep.2025.115245.Peer-Reviewed Original ResearchConceptsHuman immunodeficiency virusHIV-2Features of human immunodeficiency virusHIV-2 CAHost factor interactionsImmunodeficiency virusHIV-1Human immunodeficiency virus capsidFunctionalized liposomesCapsid proteinHigh-resolution structuresSingle-particle cryo-EMCA assemblyCA hexamersFactor interactionsViral genomeCA lattice
2024
Channel width modulates the permeability of DNA origami–based nuclear pore mimics
Feng Q, Saladin M, Wu C, Cao E, Zheng W, Zhang A, Bhardwaj P, Li X, Shen Q, Kapinos L, Kozai T, Mariappan M, Lusk C, Xiong Y, Lim R, Lin C. Channel width modulates the permeability of DNA origami–based nuclear pore mimics. Science Advances 2024, 10: eadq8773. PMID: 39536094, PMCID: PMC11559598, DOI: 10.1126/sciadv.adq8773.Peer-Reviewed Original ResearchStructural insights into PPP2R5A degradation by HIV-1 Vif
Hu Y, Delviks-Frankenberry K, Wu C, Arizaga F, Pathak V, Xiong Y. Structural insights into PPP2R5A degradation by HIV-1 Vif. Nature Structural & Molecular Biology 2024, 31: 1492-1501. PMID: 38789685, PMCID: PMC12221852, DOI: 10.1038/s41594-024-01314-6.Peer-Reviewed Original ResearchHost-virus protein interactionsCullin RING E3 ubiquitin ligasesInduced G2/M cell cycle arrestSets of proteinsG2/M cell cycle arrestSubstrate-binding siteCryogenic-electron microscopy structuresProtein phosphatase 2ADegradation-independent mechanismCell cycle arrestUbiquitin ligaseProtein interactionsPhosphatase 2AAntiviral proteinCycle arrestDegradation-dependentA-resolutionHIV-1 VifPPP2R5AStructural insightsDiverse interactionsProteinCellular studiesPhosphatase activityPotential target
2023
Abl2 repairs microtubules and phase separates with tubulin to promote microtubule nucleation
Duan D, Lyu W, Chai P, Ma S, Wu K, Wu C, Xiong Y, Sestan N, Zhang K, Koleske A. Abl2 repairs microtubules and phase separates with tubulin to promote microtubule nucleation. Current Biology 2023, 33: 4582-4598.e10. PMID: 37858340, PMCID: PMC10877310, DOI: 10.1016/j.cub.2023.09.018.Peer-Reviewed Original ResearchConceptsCOS-7 cellsMT nucleationMT latticeFamily kinasesTubulin recruitmentLiquid-liquid phase separationTubulin C-terminal tailsCryo-EM analysisC-terminal tailAbl family kinasesWild-type cellsC-terminal halfRescue frequencyGenetic experimentsNeuronal morphogenesisMicrotubule nucleationSplice isoformsMicrotubule dynamicsNocodazole treatmentMolecular mechanismsAxon guidanceCell migrationDamage sitesABL2MT assemblyThe capsid lattice engages a bipartite NUP153 motif to mediate nuclear entry of HIV-1 cores
Shen Q, Kumari S, Xu C, Jang S, Shi J, Burdick R, Levintov L, Xiong Q, Wu C, Devarkar S, Tian T, Tripler T, Hu Y, Yuan S, Temple J, Feng Q, Lusk C, Aiken C, Engelman A, Perilla J, Pathak V, Lin C, Xiong Y. The capsid lattice engages a bipartite NUP153 motif to mediate nuclear entry of HIV-1 cores. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2202815120. PMID: 36943880, PMCID: PMC10068764, DOI: 10.1073/pnas.2202815120.Peer-Reviewed Original ResearchConceptsHIV-1 capsidC-terminal tail regionTriple arginine motifNuclear pore complexPhenylalanine-glycine motifsBipartite motifNuclear importPore complexNuclear poresNuclear entryNup153Capsid latticeInteraction moduleProtein latticeCA assemblyCA hexamersIntact capsidsNucleoporinsHIV-1 coreMotifCapsidTail regionIntact formInfection studiesMechanistic evidenceEnrich and switch: IP6 and maturation of HIV-1 capsid
Wu C, Xiong Y. Enrich and switch: IP6 and maturation of HIV-1 capsid. Nature Structural & Molecular Biology 2023, 30: 239-241. PMID: 36849641, PMCID: PMC10033439, DOI: 10.1038/s41594-023-00940-w.Commentaries, Editorials and LettersModeling HIV-1 nuclear entry with nucleoporin-gated DNA-origami channels
Shen Q, Feng Q, Wu C, Xiong Q, Tian T, Yuan S, Shi J, Bedwell G, Yang R, Aiken C, Engelman A, Lusk C, Lin C, Xiong Y. Modeling HIV-1 nuclear entry with nucleoporin-gated DNA-origami channels. Nature Structural & Molecular Biology 2023, 30: 425-435. PMID: 36807645, PMCID: PMC10121901, DOI: 10.1038/s41594-023-00925-9.Peer-Reviewed Original ResearchConceptsNuclear pore complexHIV-1 nuclear entryNuclear entryNuclear importNPC central channelPore complexHost nucleusCapsid dockingVirus genomeAffinity gradientNup153Central channelMechanistic insightsMolecular interactionsCapsidNucleoporinsNup358Nup62GenomeNucleusVirusDockingVirus-1 infectionImportComplexesAbl2 mediates microtubule nucleation and repair via tubulin co-condensation
Duan D, Lyu W, Wu K, Wu C, Xiong Y, Koleske A. Abl2 mediates microtubule nucleation and repair via tubulin co-condensation. Biophysical Journal 2023, 122: 124a. DOI: 10.1016/j.bpj.2022.11.839.Peer-Reviewed Original Research