2016
Quantitative imaging of cell membrane-associated effective mass density using Photonic Crystal Enhanced Microscopy (PCEM)
Zhuo Y, Choi J, Marin T, Yu H, Harley B, Cunningham B. Quantitative imaging of cell membrane-associated effective mass density using Photonic Crystal Enhanced Microscopy (PCEM). Progress In Quantum Electronics 2016, 50: 1-18. PMID: 28649149, PMCID: PMC5479321, DOI: 10.1016/j.pquantelec.2016.10.001.Peer-Reviewed Original ResearchTight spatial confinementNarrow resonance peakEffective mass densityLabel-free live cell imagingAxial spatial resolutionPhotonic crystal biosensorPlasma membraneResonance peakMass densityEvanescent fieldSpatial confinementCell adhesionPhenotype of single cellsLight sourcesSpatial resolutionNon-coherent light sourceLive cell imagingComplex spatiotemporal informationCellular materialCellular processesQuantitative imagingMechanical propertiesCell surface interfaceMigration eventsMatrix material
2011
Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer
Seong J, Ouyang M, Kim T, Sun J, Wen P, Lu S, Zhuo Y, Llewellyn N, Schlaepfer D, Guan J, Chien S, Wang Y. Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer. Nature Communications 2011, 2: 406. PMID: 21792185, PMCID: PMC3373894, DOI: 10.1038/ncomms1414.Peer-Reviewed Original ResearchConceptsFocal adhesion kinase activationFocal adhesion kinaseDetergent-resistant membranesMembrane microdomainsSubcellular localization of focal adhesion kinaseLocalization of focal adhesion kinaseSrc activationCell adhesion to extracellular matrix proteinsAdhesion to extracellular matrix proteinsDetergent-resistant membrane microdomainsPlatelet-derived growth factorResponse to different physiological stimuliFluorescence resonance energy transferExtracellular matrix proteinsFAK activationSubcellular localizationCellular processesSubcellular compartmentsResonance energy transferKinase activityPlasma membraneCell adhesionMatrix proteinsFRET responseMicrodomains