2014
A Novel Multiple Hypothesis Based Particle Tracking Method for Clathrin Mediated Endocytosis Analysis Using Fluorescence Microscopy
Liang L, Shen H, De Camilli P, Duncan JS. A Novel Multiple Hypothesis Based Particle Tracking Method for Clathrin Mediated Endocytosis Analysis Using Fluorescence Microscopy. IEEE Transactions On Image Processing 2014, 23: 1844-1857. PMID: 24808351, PMCID: PMC4373089, DOI: 10.1109/tip.2014.2303633.Peer-Reviewed Original ResearchObjective comparison of particle tracking methods
Chenouard N, Smal I, de Chaumont F, Maška M, Sbalzarini IF, Gong Y, Cardinale J, Carthel C, Coraluppi S, Winter M, Cohen AR, Godinez WJ, Rohr K, Kalaidzidis Y, Liang L, Duncan J, Shen H, Xu Y, Magnusson KE, Jaldén J, Blau HM, Paul-Gilloteaux P, Roudot P, Kervrann C, Waharte F, Tinevez JY, Shorte SL, Willemse J, Celler K, van Wezel GP, Dan HW, Tsai YS, de Solórzano C, Olivo-Marin JC, Meijering E. Objective comparison of particle tracking methods. Nature Methods 2014, 11: 281-289. PMID: 24441936, PMCID: PMC4131736, DOI: 10.1038/nmeth.2808.Peer-Reviewed Original ResearchA role of OCRL in clathrin-coated pit dynamics and uncoating revealed by studies of Lowe syndrome cells
Nández R, Balkin DM, Messa M, Liang L, Paradise S, Czapla H, Hein MY, Duncan JS, Mann M, De Camilli P. A role of OCRL in clathrin-coated pit dynamics and uncoating revealed by studies of Lowe syndrome cells. ELife 2014, 3: e02975. PMID: 25107275, PMCID: PMC4358339, DOI: 10.7554/elife.02975.Peer-Reviewed Original ResearchMeSH KeywordsCells, CulturedClathrinClathrin-Coated VesiclesCoated Pits, Cell-MembraneEndocytosisFibroblastsHEK293 CellsHeLa CellsHumansLuminescent ProteinsMicroscopy, ConfocalMicroscopy, ElectronMicroscopy, FluorescenceMutationOculocerebrorenal SyndromePhosphatidylinositol PhosphatesPhosphoric Monoester HydrolasesProtein BindingProteomeProteomicsRNA InterferenceSorting Nexins
2011
3-D Reconstruction of Microtubules from Multi-Angle Total Internal Reflection Fluorescence Microscopy Using Bayesian Framework
Yang Q, Karpikov A, Toomre D, Duncan JS. 3-D Reconstruction of Microtubules from Multi-Angle Total Internal Reflection Fluorescence Microscopy Using Bayesian Framework. IEEE Transactions On Image Processing 2011, 20: 2248-2259. PMID: 21324778, DOI: 10.1109/tip.2011.2114359.Peer-Reviewed Original ResearchConceptsEvanescent fieldDifferent penetration depthsTIRF imagesPenetration depthSamples of microtubulesLaser beamTotal internal reflection fluorescence microscopyAxial resolutionReflection fluorescence microscopyLarge radiusIncident angleMulti-angle total internal reflection fluorescence microscopyElectron microscopy imagesTIRF dataSmall radiusTracking of microtubulesMicroscopy imagesFluorescence microscopyMicroscopyRadiusReconstruction resultsCurvilinear characteristicsZ-dimensionExperimental calibrationMicrotubule curvatureAn Expectation Maximization Based Method for Subcellular Particle Tracking Using Multi-angle TIRF Microscopy
Liang L, Shen H, De Camilli P, Toomre DK, Duncan JS. An Expectation Maximization Based Method for Subcellular Particle Tracking Using Multi-angle TIRF Microscopy. Lecture Notes In Computer Science 2011, 14: 629-636. PMID: 22003671, PMCID: PMC3648983, DOI: 10.1007/978-3-642-23623-5_79.Peer-Reviewed Original Research
2010
Estimation of 3D Geometry of Microtubules Using Multi-angle Total Internal Reflection Fluorescence Microscopy
Yang Q, Karpikov A, Toomre D, Duncan J. Estimation of 3D Geometry of Microtubules Using Multi-angle Total Internal Reflection Fluorescence Microscopy. Lecture Notes In Computer Science 2010, 13: 538-545. PMID: 20879357, DOI: 10.1007/978-3-642-15745-5_66.Peer-Reviewed Original ResearchConceptsTotal internal reflection fluorescence microscopyReflection fluorescence microscopyTotal internal reflection fluorescence microscopy imagesPtK2 cellsMicrotubulesFluorescence microscopyTIRF imagesImportant biological parametersTIRF dataFluorescence microscopy imagesMulti-angle total internal reflection fluorescence microscopyBiological parametersMicrotubule curvatureBiological samples
2008
Motion tracking of the outer tips of microtubules
Hadjidemetriou S, Toomre D, Duncan J. Motion tracking of the outer tips of microtubules. Medical Image Analysis 2008, 12: 689-702. PMID: 18571462, DOI: 10.1016/j.media.2008.04.004.Peer-Reviewed Original ResearchConceptsMicrotubule assemblyCoordination of mitosisTransport of chromosomesHigh throughput quantitative studiesNumerous critical rolesMicrotubule tipsLiving cellsCell migrationStructural tracksConfocal microscopyEpifluorescent microscopyNeurodegenerative diseasesCritical roleCell pathologyMicrotubulesAssemblyCellsSequenceOuter tipChromosomesOrganellesMitosisAbnormal functionCytoplasmVesicles
2005
Segmentation and 3D Reconstruction of Microtubules in Total Internal Reflection Fluorescence Microscopy (TIRFM)
Hadjidemetriou S, Toomre D, Duncan JS. Segmentation and 3D Reconstruction of Microtubules in Total Internal Reflection Fluorescence Microscopy (TIRFM). Lecture Notes In Computer Science 2005, 8: 761-769. PMID: 16685915, DOI: 10.1007/11566465_94.Peer-Reviewed Original Research