2022
Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules
Stravalaci M, Pagani I, Paraboschi E, Pedotti M, Doni A, Scavello F, Mapelli S, Sironi M, Perucchini C, Varani L, Matkovic M, Cavalli A, Cesana D, Gallina P, Pedemonte N, Capurro V, Clementi N, Mancini N, Invernizzi P, Bayarri-Olmos R, Garred P, Rappuoli R, Duga S, Bottazzi B, Uguccioni M, Asselta R, Vicenzi E, Mantovani A, Garlanda C. Recognition and inhibition of SARS-CoV-2 by humoral innate immunity pattern recognition molecules. Nature Immunology 2022, 23: 275-286. PMID: 35102342, DOI: 10.1038/s41590-021-01114-w.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsC-Reactive ProteinCase-Control StudiesChlorocebus aethiopsComplement ActivationCoronavirus Nucleocapsid ProteinsCOVID-19FemaleGlycosylationHEK293 CellsHost-Pathogen InteractionsHumansImmunity, HumoralMaleMannose-Binding LectinPhosphoproteinsPolymorphism, GeneticProtein BindingReceptors, Pattern RecognitionSARS-CoV-2Serum Amyloid P-ComponentSignal TransductionSpike Glycoprotein, CoronavirusVero CellsConceptsFluid phase pattern recognition moleculesPattern recognition moleculesHumoral fluid phase pattern recognition moleculesMannose-binding lectinSARS-CoV-2Pentraxin 3Severe acute respiratory syndrome coronavirus 2Acute respiratory syndrome coronavirus 2Respiratory syndrome coronavirus 2Spike proteinDisease severity biomarkersSyndrome coronavirus 2Coronavirus disease 2019Long pentraxin 3Antibody-like functionsVariants of concernRecognition moleculesHumoral armSeverity biomarkersCoronavirus 2Disease 2019Innate immunityDisease severityComplement activationTranslational implications
2020
Circulating Ficolin-2 and Ficolin-3 Form Heterocomplexes.
Jarlhelt I, Pilely K, Clausen J, Skjoedt M, Bayarri-Olmos R, Garred P. Circulating Ficolin-2 and Ficolin-3 Form Heterocomplexes. The Journal Of Immunology 2020, 204: 1919-1928. PMID: 32094208, DOI: 10.4049/jimmunol.1900694.Peer-Reviewed Original ResearchConceptsProtein complexesHeteromeric protein complexesNovel protein complexChinese hamster ovary cellsHamster ovary cellsPattern recognition moleculesHeterocomplexWestern blot analysisInnate immune systemBiological relevanceOvary cellsBlot analysisRecognition moleculesFicolin-2Soluble pattern recognition moleculesFicolin-3Lectin pathwayComplexesImmune systemCoimmunoprecipitationComplement systemMoleculesC1q/TNF-Related Protein 6 Is a Pattern Recognition Molecule That Recruits Collectin-11 from the Complement System to Ligands.
Kirketerp-Møller N, Bayarri-Olmos R, Krogfelt K, Garred P. C1q/TNF-Related Protein 6 Is a Pattern Recognition Molecule That Recruits Collectin-11 from the Complement System to Ligands. The Journal Of Immunology 2020, 204: 1598-1606. PMID: 32041782, DOI: 10.4049/jimmunol.1901316.Peer-Reviewed Original ResearchConceptsPattern recognition moleculesProtein 6Recognition moleculesLectin complement pathwayChinese hamster ovary cellsGram-negative bacteriaProtein familyHamster ovary cellsSpecific recruitmentPutative ligandCollectin-11Human lectinsOvary cellsC1q/TNF-related protein 6Innate immunityBacterial isolatesBindingPathwayEndocrine processesCTRP6Complement recognitionGlobular structureEndogenous ligandComplement pathwayEnzymeComplement related pattern recognition molecules as markers of short-term mortality in intensive care patients
Hansen C, Bayarri-Olmos R, Kristensen M, Pilely K, Hellemann D, Garred P. Complement related pattern recognition molecules as markers of short-term mortality in intensive care patients. Journal Of Infection 2020, 80: 378-387. PMID: 31981636, DOI: 10.1016/j.jinf.2020.01.010.Peer-Reviewed Original ResearchConceptsIntensive care unitSeptic shockFicolin-2Ficolin-2 levelsIntensive care patientsShort-term mortalityPattern recognition moleculesPTX3 levelsCRP levelsCare patientsICU patientsAdjusted analysisCare unitChronic diseasesMBL levelsBlood samplesBaseline levelsPatientsDisease severityChances of survivalGood markerPTX3MortalityCRPSurvivors
2019
Combining MAP‐1:CD35 or MAP‐1:CD55 fusion proteins with pattern‐recognition molecules as novel targeted modulators of the complement cascade
Pérez‐Alós L, Bayarri‐Olmos R, Skjoedt M, Garred P. Combining MAP‐1:CD35 or MAP‐1:CD55 fusion proteins with pattern‐recognition molecules as novel targeted modulators of the complement cascade. The FASEB Journal 2019, 33: 12723-12734. PMID: 31469600, PMCID: PMC6902692, DOI: 10.1096/fj.201901643r.Peer-Reviewed Original ResearchConceptsMannose-binding lectinComplement cascadePattern recognition moleculesFeasible therapeutic optionLevels of C3Lectin pathwayEffective therapeutic approachTerminal effector functionsComplement activation assaysSites of inflammationComplement receptor 1Membrane-anchored regulatorTerminal complement complexTherapeutic optionsInflammatory pathologyLP activationTherapeutic approachesEffector functionsDecay-accelerating factorFicolin-3Receptor 1CD35Complement complexMAP-1Complement system
2018
Development of a Quantitative Assay for the Characterization of Human Collectin-11 (CL-11, CL-K1)
Bayarri-Olmos R, Kirketerp-Moller N, Pérez-Alós L, Skjodt K, Skjoedt M, Garred P. Development of a Quantitative Assay for the Characterization of Human Collectin-11 (CL-11, CL-K1). Frontiers In Immunology 2018, 9: 2238. PMID: 30323815, PMCID: PMC6172411, DOI: 10.3389/fimmu.2018.02238.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsAntibodies, MonoclonalCHO CellsChromatography, GelCollectinsComplement C4CricetulusEnzyme-Linked Immunosorbent AssayFreezingHumansLectinsMannose-Binding Protein-Associated Serine ProteasesProtein BindingStatistics, NonparametricSystemic Inflammatory Response SyndromeZymosanConceptsPlasma levelsSystemic inflammatory response syndromeQuantitative ELISACL-11Immunoprecipitation of serumDisseminated intravascular coagulationInflammatory response syndromeMean plasma concentrationCollectin-11Pattern recognition moleculesIntravascular coagulationResponse syndromeIntensive careUrinary schistosomiasisHealthy controlsPlasma concentrationsClinical toolHost defenseSensitive ELISAMonoclonal antibodiesELISACongenital disorderSignificant decreaseLectin pathwayZymosanChimeric Proteins Containing MAP-1 and Functional Domains of C4b-Binding Protein Reveal Strong Complement Inhibitory Capacities
Hertz C, Bayarri-Olmos R, Kirketerp-Møller N, van Putten S, Pilely K, Skjoedt M, Garred P. Chimeric Proteins Containing MAP-1 and Functional Domains of C4b-Binding Protein Reveal Strong Complement Inhibitory Capacities. Frontiers In Immunology 2018, 9: 1945. PMID: 30210498, PMCID: PMC6120983, DOI: 10.3389/fimmu.2018.01945.Peer-Reviewed Original ResearchConceptsChimeric proteinFunctional domainsNetwork of proteinsMAP-1Dependent dimerizationN-terminusPattern recognition moleculesHuman C4BPCHO cellsLectin pathwayProteinComplement systemCo-factor activitySingle inhibitorRecognition moleculesC4BPComplement inhibitory propertiesPathwayComplement cascadeNovel therapeutic approachesAdaptive immune responsesDifferent inflammatory diseasesActivationCascadeInhibitors
2016
A journey through the lectin pathway of complement—MBL and beyond
Garred P, Genster N, Pilely K, Bayarri‐Olmos R, Rosbjerg A, Jie Y, Skjoedt M. A journey through the lectin pathway of complement—MBL and beyond. Immunological Reviews 2016, 274: 74-97. PMID: 27782323, DOI: 10.1111/imr.12468.Peer-Reviewed Original ResearchConceptsMASP-3Tissue homeostasisMannose-binding lectinLectin pathwayPattern recognition moleculesCollectin-11Innate immune functionSerine proteasesRare mutationsRecognition moleculesPathwayDistinct expressionLectin complement pathwayHost defenseMASP-1ProteinSoluble pattern recognition moleculesAlternative pathwayDisease modifiersDifferent tissue compartmentsActivationExpressionBroad functionalityCommon featureImmune function
2015
Soluble Collectin-12 (CL-12) Is a Pattern Recognition Molecule Initiating Complement Activation via the Alternative Pathway
Ma Y, Hein E, Munthe-Fog L, Skjoedt M, Bayarri-Olmos R, Romani L, Garred P. Soluble Collectin-12 (CL-12) Is a Pattern Recognition Molecule Initiating Complement Activation via the Alternative Pathway. The Journal Of Immunology 2015, 195: 3365-3373. PMID: 26290605, DOI: 10.4049/jimmunol.1500493.Peer-Reviewed Original ResearchConceptsSoluble pattern recognition moleculesPattern recognition moleculesUmbilical cord plasmaVascular endothelial cellsTerminal complement complexCollectin-12Natural soluble formWestern blot analysisCord plasmaAlternative pathwaySoluble formInnate immunityComplement activationSoluble collectinsWestern blotComplement complexEndothelial cellsC3b depositionCL-12Blot analysisHuman umbilical cord plasmaElectrophoretic mobility patternsCollectin familyA. fumigatusNovel mechanism
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