2021
Vitamin E Improves Neurite Complexity by Enhancing Mitochondrial Function
Park H, Crowe-White K, Davis A, Bannerman S, Burnett G, Scott M, Broman K, Lackey K, Licznerski P, Jonas E. Vitamin E Improves Neurite Complexity by Enhancing Mitochondrial Function. Current Developments In Nutrition 2021, 5: 5140915. PMCID: PMC8181647, DOI: 10.1093/cdn/nzab049_028.Peer-Reviewed Original ResearchPost-translational cleavagePrimary hippocampal neuronsBcl-xLMitochondrial functionHippocampal neuronsNeurite complexityNeurite outgrowthOxidative stressProtein levelsPotential therapeutic roleProtein Bcl-xLVitamin E familyATP/ADP ratioNormal physiological developmentNovel functionBrain injuryTherapeutic roleReal-time PCRSholl analysisNew synapsesNeurobasal mediumATP productionNeurite injuryControl groupVitamin EMitochondrial Fission Governed by Drp1 Regulates Exogenous Fatty Acid Usage and Storage in Hela Cells
Song JE, Alves TC, Stutz B, Šestan-Peša M, Kilian N, Jin S, Diano S, Kibbey RG, Horvath TL. Mitochondrial Fission Governed by Drp1 Regulates Exogenous Fatty Acid Usage and Storage in Hela Cells. Metabolites 2021, 11: 322. PMID: 34069800, PMCID: PMC8157282, DOI: 10.3390/metabo11050322.Peer-Reviewed Original ResearchExogenous fatty acidsMitochondrial fissionMitochondrial fatty acid oxidationFatty acid oxidationFatty acid usageMitochondrial morphologyLipid dropletsAcid usageCarnitine palmitoyltransferase 1HeLa cellsDynamin-related proteinKey mitochondrial proteinsFatty acidsAcid oxidationMitochondrial proteinsLipid droplet accumulationMitochondrial dynamicsNovel functionLipid homeostasisHigh abundanceDirect roleDroplet accumulationMitochondriaFatty acid contentProteinPresynaptic Kv3 channels are required for fast and slow endocytosis of synaptic vesicles
Wu XS, Subramanian S, Zhang Y, Shi B, Xia J, Li T, Guo X, El-Hassar L, Szigeti-Buck K, Henao-Mejia J, Flavell RA, Horvath TL, Jonas EA, Kaczmarek LK, Wu LG. Presynaptic Kv3 channels are required for fast and slow endocytosis of synaptic vesicles. Neuron 2021, 109: 938-946.e5. PMID: 33508244, PMCID: PMC7979485, DOI: 10.1016/j.neuron.2021.01.006.Peer-Reviewed Original ResearchConceptsSlow endocytosisVesicle mobilizationF-actin cytoskeletonChannel mutationsPotassium channelsKv3.3 proteinsInhibits endocytosisRapid endocytosisNovel functionF-actinEndocytosisCrucial functionSynaptic vesiclesFamily channelsSynaptic transmissionDiscovery decadesMembrane potentialNeurotransmitter releaseDiverse neurological disordersIon conductanceMutationsReleasable poolMouse nerve terminalsPotassium channel mutationsPathological effects
2020
Seizure protein 6 controls glycosylation and trafficking of kainate receptor subunits GluK2 and GluK3
Pigoni M, Hsia H, Hartmann J, Rudan Njavro J, Shmueli MD, Müller SA, Güner G, Tüshaus J, Kuhn P, Kumar R, Gao P, Tran ML, Ramazanov B, Blank B, Hipgrave Ederveen A, Von Blume J, Mulle C, Gunnersen JM, Wuhrer M, Rammes G, Busche MA, Koeglsperger T, Lichtenthaler SF. Seizure protein 6 controls glycosylation and trafficking of kainate receptor subunits GluK2 and GluK3. The EMBO Journal 2020, 39: embj2019103457. PMID: 32567721, PMCID: PMC7396870, DOI: 10.15252/embj.2019103457.Peer-Reviewed Original ResearchConceptsPrimary neuronsCell surface localizationMolecular functionsKainate receptor subunit GluK2Trafficking factorsSecretory pathwayNovel functionHeterologous cellsMajor substrateSurface localizationProtein 6Alzheimer's diseaseCA1 pyramidal neuronsAcute hippocampal slicesProtease BACE1Kainate-evoked currentsGlycosylationGluK2/3Pyramidal neuronsGluK2Hippocampal slicesKainate receptorsPsychiatric disordersNervous systemPsychiatric diseases
2019
Nuclear localization of the tyrosine kinase BMX mediates VEGFR2 expression
Liu T, Li Y, Su H, Zhang H, Jones D, Zhou HJ, Ji W, Min W. Nuclear localization of the tyrosine kinase BMX mediates VEGFR2 expression. Journal Of Cellular And Molecular Medicine 2019, 24: 126-138. PMID: 31642192, PMCID: PMC6933376, DOI: 10.1111/jcmm.14663.Peer-Reviewed Original ResearchConceptsTyrosine kinase BMXVEGFR2 promoter activityPromoter activityNuclear localizationVEGFR2 promoterKinase-inactive formGene promoter activityEndothelial cellsNucleus of ECsVascular endothelial growth factor receptorSiRNA-mediated silencingAngiogenesis-related diseasesChromatin immunoprecipitationDirect transactivationSH3 domainTranscription factorsGrowth factor receptorVEGFR2 expressionNovel functionVEGFR2 transcriptionSp1Human endothelial cellsLuciferase assayEC migrationFactor receptorNoroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation
Hosmillo M, Lu J, McAllaster MR, Eaglesham JB, Wang X, Emmott E, Domingues P, Chaudhry Y, Fitzmaurice TJ, Tung MK, Panas MD, McInerney G, Locker N, Wilen CB, Goodfellow IG. Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation. ELife 2019, 8: e46681. PMID: 31403400, PMCID: PMC6739877, DOI: 10.7554/elife.46681.Peer-Reviewed Original ResearchConceptsViral positive-sense RNAFirst host factorHost factorsPositive-sense RNAPro-viral activityPositive-sense RNA virusesSense RNA virusesG3BP1 functionsRibosome recruitmentTranslation complexesTranslation initiationCRISPR screensProteomic analysisMurine norovirus infectionReplication complexSense RNANovel functionViral translationRNA virusesG3BP1Data uncoversNorovirus replicationLife cycleVPgGenus
2018
The Vibrio H-Ring Facilitates the Outer Membrane Penetration of the Polar Sheathed Flagellum
Zhu S, Nishikino T, Kojima S, Homma M, Liu J. The Vibrio H-Ring Facilitates the Outer Membrane Penetration of the Polar Sheathed Flagellum. Journal Of Bacteriology 2018, 200: 10.1128/jb.00387-18. PMID: 30104237, PMCID: PMC6182240, DOI: 10.1128/jb.00387-18.Peer-Reviewed Original ResearchConceptsPolar sheathed flagellumPeriplasmic flagellaExternal flagellaOuter membraneSheathed flagellumBacterial life cycleCryo-electron tomographyWild-type cellsInternal periplasmic flagellaMultiple peritrichous flagellaRemarkable nanomachinesFlagellar genesPeritrichous flagellaPeriplasmic spaceMost bacteriaNovel functionMolecular basisBacterial flagellaMajor organellesBacterial speciesFlagellaMembrane penetrationH-ringGenesLife cyclePTEN Regulates Non-Homologous End Joining by Epigenetic Induction of NHEJ1/XLF
Sulkowski PL, Scanlon SE, Oeck S, Glazer PM. PTEN Regulates Non-Homologous End Joining by Epigenetic Induction of NHEJ1/XLF. Molecular Cancer Research 2018, 16: molcanres.0581.2017. PMID: 29739874, PMCID: PMC6072556, DOI: 10.1158/1541-7786.mcr-17-0581.Peer-Reviewed Original ResearchConceptsDNA double-strand breaksKey DNA repair pathwaysCytotoxic DNA lesionsXRCC4-like factorPatient-derived melanomasDNA repair pathwaysDouble-strand breaksNovel regulatory roleTumor suppressor geneSuppression of PTENHistone acetyltransferasesDSB repairGenomic analysisNHEJ defectsNonhomologous endRepair pathwaysGene promoterNovel functionRegulatory acetylationNHEJ deficiencyDNA lesionsRegulatory roleSuppressor geneNHEJ DSB repairNHEJ
2017
Structural basis of host recognition and biofilm formation by Salmonella Saf pili
Zeng L, Zhang L, Wang P, Meng G. Structural basis of host recognition and biofilm formation by Salmonella Saf pili. ELife 2017, 6: e28619. PMID: 29125121, PMCID: PMC5700814, DOI: 10.7554/elife.28619.Peer-Reviewed Original ResearchConceptsHost recognitionBiofilm formationNovel functionStructure-based mutantsSelf-association activityFunctional characterizationBiofilm formation assaysStructural basisBacterial aggregationFormation assaysCell aggregationPiliAdherence assaysColonizationSAXS characterizationAssaysMutantsSAFABacterial infectionsSAFDOligomerizationCrystal structureAggregationMechanismFormation
2016
Mammalian African trypanosome VSG coat enhances tsetse’s vector competence
Aksoy E, Vigneron A, Bing X, Zhao X, O'Neill M, Wu YN, Bangs JD, Weiss BL, Aksoy S. Mammalian African trypanosome VSG coat enhances tsetse’s vector competence. Proceedings Of The National Academy Of Sciences Of The United States Of America 2016, 113: 6961-6966. PMID: 27185908, PMCID: PMC4922192, DOI: 10.1073/pnas.1600304113.Peer-Reviewed Original ResearchConceptsVariant surface glycoproteinPeritrophic matrixMammalian hostsVector competenceTranscription factor familyMidgut homeostasisTsetse midgutTrypanosome biologyFactor familyPM barrierCoat proteinNovel functionAfrican trypanosomesTsetse vectorInfection processParasite developmentAnimal trypanosomiasesAntigenic variationVSG moleculesVSG coatBiological vectorsMidgutProtozoan parasiteDisease transmissionTsetse flies
2015
Spatially resolved, highly multiplexed RNA profiling in single cells
Chen KH, Boettiger AN, Moffitt JR, Wang S, Zhuang X. Spatially resolved, highly multiplexed RNA profiling in single cells. Science 2015, 348: aaa6090. PMID: 25858977, PMCID: PMC4662681, DOI: 10.1126/science.aaa6090.Peer-Reviewed Original ResearchConceptsRNA speciesMultiplexed error-robust fluorescenceSingle-molecule imaging approachesDistinct RNA speciesIndividual cellsGene regulatory networksPairs of genesSingle cellsDistinct spatial distribution patternsUnannotated genesSingle-molecule labelingRegulatory networksNovel functionRNA profilingExpression profilesCellular behaviorCopy numberSitu hybridizationRich repertoireSpatial distribution patternsGenesDistribution patternsSpeciesCellsSpatial landscape
2014
The RAG Recombinase Dictates Functional Heterogeneity and Cellular Fitness in Natural Killer Cells
Karo JM, Schatz DG, Sun JC. The RAG Recombinase Dictates Functional Heterogeneity and Cellular Fitness in Natural Killer Cells. Cell 2014, 159: 94-107. PMID: 25259923, PMCID: PMC4371485, DOI: 10.1016/j.cell.2014.08.026.Peer-Reviewed Original ResearchConceptsRecombination-activating geneDNA damage response mediatorsInnate lymphoid cellsNatural killer cellsAntigen receptor genesCellular fitnessJawed vertebratesRAG recombinaseCellular stressInnate lymphocytesNovel functionDNA breaksKiller cellsEndonuclease activityUnexpected roleCleavage eventsAdaptive immune cellsReceptor geneReduced expressionGenesFunctional heterogeneityCellsImmune cellsResponse mediatorsFitnessAIP1 Mediates Vascular Endothelial Cell Growth Factor Receptor-3–Dependent Angiogenic and Lymphangiogenic Responses
Zhou HJ, Chen X, Huang Q, Liu R, Zhang H, Wang Y, Jin Y, Liang X, Lu L, Xu Z, Min W. AIP1 Mediates Vascular Endothelial Cell Growth Factor Receptor-3–Dependent Angiogenic and Lymphangiogenic Responses. Arteriosclerosis Thrombosis And Vascular Biology 2014, 34: 603-615. PMID: 24407031, PMCID: PMC3952062, DOI: 10.1161/atvbaha.113.303053.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsCarrier ProteinsCells, CulturedCorneaEndocytosisEndothelial CellsEndothelium, VascularEye ProteinsGuanylate KinasesHumansLymphangiogenesisMiceMice, KnockoutMicroRNAsNeuronsras GTPase-Activating ProteinsReceptors, NotchRecombinant ProteinsRetinal NeovascularizationRNA InterferenceRNA, Small InterferingVascular Endothelial Growth Factor CVascular Endothelial Growth Factor Receptor-2Vascular Endothelial Growth Factor Receptor-3ConceptsLymphatic endothelial cellsASK1-interacting protein-1VEGFR-3 signalingHuman lymphatic endothelial cellsVEGFR-3Vascular endothelial cell growth factor receptorEndothelial cellsReduced expressionDevelopmental lymphangiogenesisScaffold proteinAIP1 functionsGrowth factor receptorLymphangiogenic signalingNovel functionVEGFR-2 activityRNA knockdownCell growth factor receptorLymphangiogenic responseSimilar defectsFirst insightProtein 1Vascular endothelial cellsPathological angiogenesisSpecific deletionFactor receptorDetecting rare gene transfer events in bacterial populations
Nielsen KM, Bøhn T, Townsend JP. Detecting rare gene transfer events in bacterial populations. Frontiers In Microbiology 2014, 4: 415. PMID: 24432015, PMCID: PMC3882822, DOI: 10.3389/fmicb.2013.00415.Peer-Reviewed Original ResearchHorizontal gene transferHGT eventsHost fitnessBacterial populationsDetection of HGTNew hostPopulation sizeGene transfer eventsKey population genetics parametersStrong positive selectionPopulation genetic parametersGene transfer mechanismsPopulation genetic modelingLarge population sizesGenetic diversityRelative population sizeGenetic compatibilityHGT ratesPositive selectionGenetic variationMutational processesNovel functionDonor organismHighlight knowledge gapsStructured populations
2013
AID downregulation is a novel function of the DNMT inhibitor 5-aza-deoxycytidine
Tsai CT, Yang PM, Chern TR, Chuang SH, Lin JH, Klemm L, Müschen M, Chen CC. AID downregulation is a novel function of the DNMT inhibitor 5-aza-deoxycytidine. Oncotarget 2013, 5: 211-223. PMID: 24457556, PMCID: PMC3960202, DOI: 10.18632/oncotarget.1319.Peer-Reviewed Original ResearchConceptsActivation-induced cytidine deaminaseClass switch recombinationTumor suppressor geneHematopoietic cancer cellsAID expressionSomatic hypermutationNovel biological functionDNMT inhibitor 5Cancer cellsHost genesProteasomal degradationDNMT inhibitorsNovel functionBiological functionsInhibitor 5Suppressor geneSwitch recombinationImmunoglobulin genesCancer progressionCytidine deaminaseGenesDNMT1ZEBMolecular dockingActive siteExpanding Horizons: Ciliary Proteins Reach Beyond Cilia
Yuan S, Sun Z. Expanding Horizons: Ciliary Proteins Reach Beyond Cilia. Annual Review Of Genetics 2013, 47: 353-376. PMID: 24016188, PMCID: PMC5703194, DOI: 10.1146/annurev-genet-111212-133243.Peer-Reviewed Original ResearchMeSH KeywordsAbnormalities, MultipleAnimalsBardet-Biedl SyndromeCell MovementCerebellar DiseasesCerebellumCiliaCiliary Motility DisordersDisease Models, AnimalDNA DamageDNA RepairEncephaloceleEye AbnormalitiesFlagellaHeterotaxy SyndromeHomeostasisHumansKidney Diseases, CysticMolecular Motor ProteinsNervous SystemPolycystic Kidney DiseasesPolycystic Kidney, Autosomal DominantPolycystic Kidney, Autosomal RecessiveRetinaRetinitis Pigmentosa
2012
miR-1 and miR-206 regulate angiogenesis by modulating VegfA expression in zebrafish
Stahlhut C, Suárez Y, Lu J, Mishima Y, Giraldez AJ. miR-1 and miR-206 regulate angiogenesis by modulating VegfA expression in zebrafish. Development 2012, 139: 4356-4365. PMID: 23132244, PMCID: PMC3509730, DOI: 10.1242/dev.083774.Peer-Reviewed Original ResearchConceptsMiR-1/206Post-transcriptional modulatorsMiRNA-target interactionsMiR-1Appropriate physiological responsesRegulation of VEGFAZebrafish developmentEmbryonic developmentTarget protectorNovel functionPrecise regulationGene expressionMorphogenetic activityDevelopmental angiogenesisPutative targetsRegulate angiogenesisEssential processMiR-206Physiological responsesCellular communicationVEGFA expressionGrowth factorVascular endothelial growth factorExpressionAngiogenesisCharacterization of Lipid Droplet and Its Regulation by Caveolin‐1 in Endothelial Cells
Kuo A, Zhang X, Harrison K, Sessa W. Characterization of Lipid Droplet and Its Regulation by Caveolin‐1 in Endothelial Cells. The FASEB Journal 2012, 26: 597.1-597.1. DOI: 10.1096/fasebj.26.1_supplement.597.1.Peer-Reviewed Original ResearchDeficient endothelial cellsCav-1Lipid dropletsFatty acid uptakeLD formationCaveolin-1Putative hairpin structureSurface of LDsCav-1 null miceIntegral membrane proteinsCellular energy homeostasisPotential physiological functionsAcid uptakeEndothelial cellsLD homeostasisUnique organellesCaveolae microdomainsMembrane proteinsKey cell typesDiacylglycerol acyltransferasesNovel functionPlasma membraneBiological processesIntracellular regulationPhysiological functions
2011
A Novel Role for the AAA ATPase Spastin as a HOXA10 Transcriptional Corepressor in Ishikawa Endometrial Cells
Daftary GS, Tetrault AM, Jorgensen EM, Sarno J, Taylor HS. A Novel Role for the AAA ATPase Spastin as a HOXA10 Transcriptional Corepressor in Ishikawa Endometrial Cells. Endocrinology 2011, 25: 1539-1549. PMID: 21757506, PMCID: PMC3165913, DOI: 10.1210/me.2011-0001.Peer-Reviewed Original ResearchConceptsEmpty spiracles homeobox 2Novel roleNuclear localization signal sequenceHoxA10 target geneEMX2 expressionDrosophila empty spiraclesCorepressor complexImplantation biologyTranscriptional regulationTranscriptional corepressorTranscriptional complexChromatin immunoprecipitationHuman orthologEndometrial expressionImplantation rateSignal sequenceTranscription factorsEndometrial cellsEmpty spiraclesEmbryo receptivityIshikawa cellsNovel functionTarget genesHomeobox A10Embryo implantationNFBD1/MDC1 Regulates Cav1 and Cav2 Independently of DNA Damage and p53
Wilson KA, Colavito SA, Schulz V, Wakefield PH, Sessa W, Tuck D, Stern DF. NFBD1/MDC1 Regulates Cav1 and Cav2 Independently of DNA Damage and p53. Molecular Cancer Research 2011, 9: 766-781. PMID: 21551225, PMCID: PMC3901581, DOI: 10.1158/1541-7786.mcr-10-0317.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsAtaxia Telangiectasia Mutated ProteinsCaveolin 1Caveolin 2Cell AdhesionCell Cycle ProteinsCell Line, TumorCells, CulturedChromatinDNA DamageDNA RepairDNA-Binding ProteinsFibroblastsGene Knockdown TechniquesHistonesHumansMiceNuclear ProteinsProtein Serine-Threonine KinasesRNA, MessengerSignal TransductionTrans-ActivatorsTranscription, GeneticTumor Suppressor Protein p53Tumor Suppressor ProteinsConceptsDNA damage checkpoint signalingNFBD1 knockdownDNA damageNFBD1/MDC1Focal adhesion signalingDNA repair factorsDNA damage responseP53-mediated transcriptionAdhesion signalingCheckpoint signalingRepair factorsResponsive transcriptionDamage responseMitogenic signalingNFBD1DNA repairNovel functionTransactivation activityGene pathwaysAtaxia telangiectasiaMicroarray analysisSimilar phenotypeERK phosphorylationGenesTranscription
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