2025
Human sperm KSper is physiologically activated by intracellular pH alkalization and CatSper-mediated Ca2+ signaling
Kang H, Wang H, Wu J, Zhang J, Zhang X, Zeng X. Human sperm KSper is physiologically activated by intracellular pH alkalization and CatSper-mediated Ca2+ signaling. Journal Of Biological Chemistry 2025, 110752. PMID: 40992664, DOI: 10.1016/j.jbc.2025.110752.Peer-Reviewed Original ResearchCytosolic Ca<sup>2+</sup>Physiological agonistsHyperpolarization of membrane potentialCurrent-clamp recordingsSperm fertilizing capacityChanges of intracellular pHResponse to physiological stimuliCa2+ signalingDose-dependent mannerPipette solutionHuman spermHyperpolarizing effectKSperPharmacological inhibitionCatSperFertilizing capacityElectrophysiological recordingsIntracellular pHHyperpolarizationPhysiological stimuliMembrane potentialRegulatory mechanismsLow pH sensitivitySpermPH alkalizationIn vivo 3D myocardial membrane potential mapping in humans using PET/MRI
Bijari F, Han P, Marin T, Lee W, Chemli Y, Gertsenshteyn I, Mounime I, Djebra Y, Chi D, Normandin M, Ma C, Fakhri G. In vivo 3D myocardial membrane potential mapping in humans using PET/MRI. EJNMMI Research 2025, 15: 93. PMID: 40715686, PMCID: PMC12297085, DOI: 10.1186/s13550-025-01287-7.Peer-Reviewed Original ResearchMembrane potentialExtracellular volume fraction measurementsExtracellular volume fraction mappingBolus-plus-infusion protocolT1 mapping sequencesVolume of distributionWritten Informed ConsentCardiac PET/MR imagingRigid image registrationHumans in vivoContrast agent injectionPET motion correctionFree breathingTracer volume of distributionImage registrationBolus injectionCardiac MRMitochondrial membrane potentialCardiac diseaseHealthy subjectsPET/MR imagingImaging studiesTreatment monitoringAgent injectionPET tracers1840-P: Pyruvate Kinase Bioenergetically Restricts OXPHOS in Beta Cells and Myotubules by Inducing Mitochondrial Membrane Hyperpolarization
DAVIDSON E, KIBBEY R. 1840-P: Pyruvate Kinase Bioenergetically Restricts OXPHOS in Beta Cells and Myotubules by Inducing Mitochondrial Membrane Hyperpolarization. Diabetes 2025, 74 DOI: 10.2337/db25-1840-p.Peer-Reviewed Original ResearchMitochondrial membrane potentialPyruvate kinaseHyperpolarization of mitochondrial membrane potentialRespiratory suppressionMitochondrial membrane potential differenceMitochondrial membrane hyperpolarizationCytosolic ATP/ADP ratioComplex V activityDigestive and Kidney DiseasesINS-1ATP hydrolysisATP synthesisPhosphoenolpyruvateMembrane hyperpolarizationPK substratesBlocking electron transportOXPHOSKidney diseaseATP/ADP ratioBeta cellsHyperpolarizationMembrane potentialPK activityState 3OligosP-551. The Effects of Antiretroviral Therapy on Immune Activation, Inflammation, Cellular Changes and Clinical Outcomes in Elite Controllers
Chan E, Zhou L, Emu B, Paintsil E, Barakat L. P-551. The Effects of Antiretroviral Therapy on Immune Activation, Inflammation, Cellular Changes and Clinical Outcomes in Elite Controllers. Open Forum Infectious Diseases 2025, 12: ofae631.750. PMCID: PMC11777457, DOI: 10.1093/ofid/ofae631.750.Peer-Reviewed Original ResearchEffects of antiretroviral therapyAntiretroviral therapyElite controllersViral loadIL-6Inflammatory markersImmune activationReactive oxygen speciesReactive oxygen species productionAdverse effects of ARTMitochondrial membrane potentialBenefits of antiretroviral therapyAntiretroviral Therapy ToxicitiesInflammatory markers IL-6Antiretroviral therapy groupHIV viral loadMembrane potentialCurrent published dataUtilization of antiretroviral therapyCellular inflammatory markersCompared to baselineViremic controllersClinical outcomesCRP decreaseLevels of mtDNA deletions
2024
Mitochondria as determinants of reproductive senescence and competence: implications for diagnosis of embryo competence in assisted reproduction
Yildirim R, Seli E. Mitochondria as determinants of reproductive senescence and competence: implications for diagnosis of embryo competence in assisted reproduction. Human Reproduction 2024, 39: 2160-2170. PMID: 39066612, DOI: 10.1093/humrep/deae171.Peer-Reviewed Original ResearchMultiple quality control mechanismsMitochondrial unfolded protein responseMitochondrial DNA copy numberUnfolded protein responseQuality control mechanismsDNA copy numberMitochondrial networkRegulate calcium signalingMitochondrial dynamicsReproductive successMitochondrial quantityProtein responseDinucleotide contentModulate apoptosisOxidative phosphorylationCellular pathwaysCopy numberMitochondrial dysfunctionMitochondriaCalcium signalingEmbryo competenceMembrane potentialAssisted reproductionDiagnostic targetsReproductive senescence
2023
Mitochondria-targeted cyclometalated iridium-β-carboline complexes as potent non-small cell lung cancer therapeutic agents
Chen J, Guo X, Li D, Tang H, Gao J, Yu W, Zhu X, Sun Z, Huang Z, Chen L. Mitochondria-targeted cyclometalated iridium-β-carboline complexes as potent non-small cell lung cancer therapeutic agents. Metallomics 2023, 15: mfad035. PMID: 37204038, DOI: 10.1093/mtomcs/mfad035.Peer-Reviewed Original ResearchConceptsLoss of mitochondrial membrane potentialIridium complexesDeath of A549 cellsMitochondrial membrane potentialA549 cellsDepletion of cellular ATPElevation of reactive oxygen speciesNSCLC therapeutic agentsMitochondrial eventsCaspase pathwayCancer therapeutic agentsReactive oxygen speciesPotential antitumor effectsCellular ATPDevelopment of antitumor drugsMulticellular tumor spheroid modelAntitumor effectNon-smallTumor spheroid modelTumor growthMembrane potentialIridiumOxygen speciesTherapeutic agentsAntitumor drugsMitochondrial stress response gene Clpp deficiency impairs oocyte competence and deteriorate cyclophosphamide-induced ovarian damage in young mice
Li G, Gu J, Zhou X, Wu T, Li X, Hua R, Hai Z, Xiao Y, Su J, Yeung W, Liu K, Guo C, Wang T. Mitochondrial stress response gene Clpp deficiency impairs oocyte competence and deteriorate cyclophosphamide-induced ovarian damage in young mice. Frontiers In Endocrinology 2023, 14: 1122012. PMID: 37033217, PMCID: PMC10081448, DOI: 10.3389/fendo.2023.1122012.Peer-Reviewed Original ResearchConceptsCyclophosphamide-induced ovarian damageOvarian damageOocyte-specific deletionOocyte competencePremature ovarian insufficiencyImpaired oocyte competenceChemotherapeutic drug cyclophosphamideCaseinolytic peptidase PDecreased mitochondrial membrane potentialAneuploidy rateMII oocytesCTX treatmentElevated ROS levelsOvarian insufficiencyEarly folliculogenesisKnockout miceYoung miceMitochondrial membrane potentialMouse modelDrug cyclophosphamideTreat cancerMiceOocytesSpindle rateMembrane potential
2022
Development of an Open Microfluidic Platform for Oocyte One-Stop Vitrification with Cryotop Method
Miao S, Guo C, Jiang Z, Wei H, Jiang X, Gu J, Hai Z, Wang T, Liu Y. Development of an Open Microfluidic Platform for Oocyte One-Stop Vitrification with Cryotop Method. Biosensors 2022, 12: 766. PMID: 36140151, PMCID: PMC9496857, DOI: 10.3390/bios12090766.Peer-Reviewed Original ResearchConceptsCryotop methodMitochondrial membrane potentialMicrofluidic systemReactive oxygen speciesLevels of oocytesEffect of vitrificationCryoprotective agentsOocyte vitrificationRisk of cell lossOpen microfluidic systemTreated oocytesFertility preservationCell transferCryotopReproductive centerCell lossSurvival rateOpen microfluidic chipOocytesAssisted reproductionSufficient cooling rateMembrane potentialProcessing chipOpen microfluidic platformAutomatic microfluidic systemPET imaging of mitochondrial function in acute doxorubicin-induced cardiotoxicity: a proof-of-principle study
Detmer F, Alpert N, Moon S, Dhaynaut M, Guerrero J, Guehl N, Xing F, Brugarolas P, Shoup T, Normandin M, Pelletier-Galarneau M, El Fakhri G, Petibon Y. PET imaging of mitochondrial function in acute doxorubicin-induced cardiotoxicity: a proof-of-principle study. Scientific Reports 2022, 12: 6122. PMID: 35414642, PMCID: PMC9005533, DOI: 10.1038/s41598-022-10004-6.Peer-Reviewed Original ResearchConceptsLeft anterior descending coronary arteryDoxorubicin-induced cardiotoxicityCardiac membrane potentialDoxorubicin infusionMembrane potentialAnimal modelsAcute doxorubicin-induced cardiotoxicityLeft anterior descending coronary artery territoryAcute cardiotoxic effectsAnterior descending coronary arteryControl saline infusionDescending coronary arteryDoxorubicin doseSaline infusionTest infusionCardiotoxic effectsMitochondrial membrane potentialInfusion catheterCoronary arteryInfusionMitochondrial functionDoxorubicinMyocardial areaPET imagingIntracoronary catheterPrestin-Mediated Frequency Selectivity Does not Cover Ultrahigh Frequencies in Mice
Li J, Liu S, Song C, Zhu T, Zhao Z, Sun W, Wang Y, Song L, Xiong W. Prestin-Mediated Frequency Selectivity Does not Cover Ultrahigh Frequencies in Mice. Neuroscience Bulletin 2022, 38: 769-784. PMID: 35279808, PMCID: PMC9276951, DOI: 10.1007/s12264-022-00839-4.Peer-Reviewed Original ResearchConceptsOuter hair cellsCation channel blockerCochlear outer hair cellsPrestin knockout miceAudiometric measurementsChannel blockersPharmacogenetic manipulationCochlear sensitivityMiceSpecific ablationHair cellsTarget cellsBehavior testsHearingMembrane potentialLow-pass featureAblationUltrasonic hearingCellsPrestinBlockersMembrane resonance
2021
Quantification of Myocardial Mitochondrial Membrane Potential Using PET
Pelletier-Galarneau M, Detmer F, Petibon Y, Normandin M, Ma C, Alpert N, El Fakhri G. Quantification of Myocardial Mitochondrial Membrane Potential Using PET. Current Cardiology Reports 2021, 23: 70. PMID: 33970353, PMCID: PMC8443083, DOI: 10.1007/s11886-021-01500-8.Peer-Reviewed Original ResearchImaging the transmembrane and transendothelial sodium gradients in gliomas
Khan MH, Walsh JJ, Mihailović JM, Mishra SK, Coman D, Hyder F. Imaging the transmembrane and transendothelial sodium gradients in gliomas. Scientific Reports 2021, 11: 6710. PMID: 33758290, PMCID: PMC7987982, DOI: 10.1038/s41598-021-85925-9.Peer-Reviewed Original ResearchPresynaptic Kv3 channels are required for fast and slow endocytosis of synaptic vesicles
Wu XS, Subramanian S, Zhang Y, Shi B, Xia J, Li T, Guo X, El-Hassar L, Szigeti-Buck K, Henao-Mejia J, Flavell RA, Horvath TL, Jonas EA, Kaczmarek LK, Wu LG. Presynaptic Kv3 channels are required for fast and slow endocytosis of synaptic vesicles. Neuron 2021, 109: 938-946.e5. PMID: 33508244, PMCID: PMC7979485, DOI: 10.1016/j.neuron.2021.01.006.Peer-Reviewed Original ResearchConceptsSlow endocytosisVesicle mobilizationF-actin cytoskeletonChannel mutationsPotassium channelsKv3.3 proteinsInhibits endocytosisRapid endocytosisNovel functionF-actinEndocytosisCrucial functionSynaptic vesiclesFamily channelsSynaptic transmissionDiscovery decadesMembrane potentialNeurotransmitter releaseDiverse neurological disordersIon conductanceMutationsReleasable poolMouse nerve terminalsPotassium channel mutationsPathological effectsATPIF1 maintains normal mitochondrial structure which is impaired by CCM3 deficiency in endothelial cells
Wang K, Chen H, Zhou Z, Zhang H, Zhou HJ, Min W. ATPIF1 maintains normal mitochondrial structure which is impaired by CCM3 deficiency in endothelial cells. Cell & Bioscience 2021, 11: 11. PMID: 33422124, PMCID: PMC7796565, DOI: 10.1186/s13578-020-00514-z.Peer-Reviewed Original ResearchActivation of mitophagyHuman umbilical vein endothelial cellsNormal mitochondrial structureMorphology of mitochondriaRNA-seq screeningMitochondrial membrane potentialCRISPR-Cas9 SystemCerebral cavernous malformationsEndothelial cellsExpression of KLF4Destruction of mitochondriaUmbilical vein endothelial cellsMitochondrial structureSignaling pathwaysVein endothelial cellsMitochondriaATPIF1MitophagyEndothelial progenitor cellsProgenitor cellsCell proliferationMembrane potentialKLF4PathwayProtein
2020
Disrupting polycystin-2 EF hand Ca2+ affinity does not alter channel function or contribute to polycystic kidney disease
Vien TN, Ng LCT, Smith JM, Dong K, Krappitz M, Gainullin VG, Fedeles S, Harris PC, Somlo S, DeCaen PG. Disrupting polycystin-2 EF hand Ca2+ affinity does not alter channel function or contribute to polycystic kidney disease. Journal Of Cell Science 2020, 133: jcs255562. PMID: 33199522, PMCID: PMC7774883, DOI: 10.1242/jcs.255562.Peer-Reviewed Original ResearchConceptsAutosomal dominant polycystic kidney diseasePolycystic kidney diseaseKidney diseaseDominant polycystic kidney diseaseChannel functionPhysiological membrane potentialsPolycystin-2Primary ciliaDuct cellsNew mouseChannel activityDiseaseIon channelsDistinct mutationsInternal CaMembrane potentialChannel regulationHand associationEF-hand Ca2Regulatory mechanismsMutationsMiceIn vivo quantitative mapping of human mitochondrial cardiac membrane potential: a feasibility study
Pelletier-Galarneau M, Petibon Y, Ma C, Han P, Kim S, Detmer F, Yokell D, Guehl N, Normandin M, El Fakhri G, Alpert N. In vivo quantitative mapping of human mitochondrial cardiac membrane potential: a feasibility study. European Journal Of Nuclear Medicine And Molecular Imaging 2020, 48: 414-420. PMID: 32719915, PMCID: PMC7839097, DOI: 10.1007/s00259-020-04878-9.Peer-Reviewed Original ResearchConceptsMembrane potentialBolus injectionHealthy subjectsHematocrit levelsSerial venous blood samplesT1 mapping imagesVenous blood samplesExtracellular space fractionMethodsThirteen healthy subjectsDynamic PET acquisitionCellular membrane potentialGadolinium injectionPrognostic informationVentricular arrhythmiasHeart failureMyocardial hypertrophyMitochondrial membrane potentialCardiac diseaseIn vitro assessmentTherapy monitoringBlood concentrationsPET acquisitionImaging protocolBlood samplesPlasma tracer concentrationThe new role of F1Fo ATP synthase in mitochondria-mediated neurodegeneration and neuroprotection
Mnatsakanyan N, Jonas EA. The new role of F1Fo ATP synthase in mitochondria-mediated neurodegeneration and neuroprotection. Experimental Neurology 2020, 332: 113400. PMID: 32653453, PMCID: PMC7877222, DOI: 10.1016/j.expneurol.2020.113400.Peer-Reviewed Original ResearchConceptsMitochondrial inner membraneATP synthaseInner membraneOxidative phosphorylationF1Fo-ATP synthaseUnique rotational mechanismMitochondrial inner membrane potentialEfficient cellular metabolismInner membrane potentialMitochondrial permeability transition porePermeability transition poreUnique regulatorAbundant proteinsNew roleCellular metabolismCell lifeProton translocationATP synthesisTransition poreCell survivalElectrochemical gradientCertain pathophysiological conditionsSynthaseATPMembrane potentialIn-vivo Imaging of Mitochondrial Depolarization of Myocardium With Positron Emission Tomography and a Proton Gradient Uncoupler
Alpert N, Pelletier-Galarneau M, Kim S, Petibon Y, Sun T, Ramos-Torres K, Normandin M, Fakhri G. In-vivo Imaging of Mitochondrial Depolarization of Myocardium With Positron Emission Tomography and a Proton Gradient Uncoupler. Frontiers In Physiology 2020, 11: 491. PMID: 32499721, PMCID: PMC7243673, DOI: 10.3389/fphys.2020.00491.Peer-Reviewed Original ResearchMitochondrial membrane potentialPositron emission tomographyProton gradient uncouplerMembrane potentialIntracoronary infusionEmission tomographyPositron emission tomography scanBolus plus infusionVolume of distributionMitochondrial membraneCellular membrane potentialBAM15InfusionPreliminary dose-response experimentsMaximum depolarizationDose-response experimentsIn vivo imagingSecular equilibriumCytoprotective effects of (E)-N-(2-(3, 5-dimethoxystyryl) phenyl) furan-2-carboxamide (BK3C231) against 4-nitroquinoline 1-oxide-induced damage in CCD-18Co human colon fibroblast cells
Tan H, Thomas N, Inayat-Hussain S, Chan K. Cytoprotective effects of (E)-N-(2-(3, 5-dimethoxystyryl) phenyl) furan-2-carboxamide (BK3C231) against 4-nitroquinoline 1-oxide-induced damage in CCD-18Co human colon fibroblast cells. PLOS ONE 2020, 15: e0223344. PMID: 32365104, PMCID: PMC7197815, DOI: 10.1371/journal.pone.0223344.Peer-Reviewed Original ResearchConceptsNormal human colon fibroblastMitochondrial damageMitochondrial membrane potentialNitrosative stressHuman colon fibroblastsN-furanDNA strand breaksCellular DNACCD-18Co cellsCytoprotective effectsStrand breaksColon fibroblastsProtein expressionDNAMembrane potentialFibroblast cellsMicronucleus formationOxidative stressCytoprotective potentialCell modelGroup of chemicalsCellsNitric oxide levelsPotential chemopreventive activityGlutathione levelsMaturation of Voltage-induced Shifts in SLC26a5 (Prestin) Operating Point during Trafficking and Membrane Insertion
Zhai F, Song L, Bai JP, Dai C, Navaratnam D, Santos-Sacchi J. Maturation of Voltage-induced Shifts in SLC26a5 (Prestin) Operating Point during Trafficking and Membrane Insertion. Neuroscience 2020, 431: 128-133. PMID: 32061780, PMCID: PMC8720582, DOI: 10.1016/j.neuroscience.2020.02.003.Peer-Reviewed Original ResearchConceptsMembrane motor proteinVoltage-dependent proteinsMembrane insertionOuter hair cellsMotor proteinsPrestin densityHEK cell linesConformational changesBiophysical forcesOHC electromotilityProteinCell linesMembrane potentialCooperative interactionsPrestinHair cellsCochlear amplificationTransmembrane voltageNonlinear capacitanceMammalsTraffickingVoltage-induced shiftElectromotilityMaturationMembrane
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